5epo
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The three-dimensional structure of Clostridium absonum 7alpha-hydroxysteroid dehydrogenase== |
| + | <StructureSection load='5epo' size='340' side='right' caption='[[5epo]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5epo]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EPO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EPO FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=TUD:TAUROCHENODEOXYCHOLIC+ACID'>TUD</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5epo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5epo OCA], [http://pdbe.org/5epo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5epo RCSB], [http://www.ebi.ac.uk/pdbsum/5epo PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | 7alpha-hydroxysteroid dehydrogenase (7alpha-HSDH) can catalyse the oxidation of C7 alpha-OH of the steroid nucleus in the bile acid metabolism. In the paper we determined the crystal structure of 7alpha-HSDH from Clostridium absonum (CA 7alpha-HSDH) complexed with taurochenodeoxycholic acid (TCDCA) and NADP(+) by X-ray diffraction, which, as a tetramer, possesses the typical alpha/beta folding pattern. The four subunits of an asymmetric unit lie in the fact that there are the stable hydrophobic interactions between Q-axis-related subunits. Significantly, we captured an active state of the NADP(+), confirming that nicotinamide moiety of NADP(+) act as electron carrier in the dehydrogenation. On the basis of crystal structure analysis, site-directed mutagenesis and MD simulation, furthermore, we find that the guanidinium of Arg38 can form the stable cation-pi interaction with the adenine ring of NADP(+), and the cation-pi interaction and hydrogen bonds between Arg38 and NADP(+) have a significant anchor effect on the cofactor binding to CA 7alpha-HSDH. | ||
| - | + | The three-dimensional structure of Clostridium absonum 7alpha-hydroxysteroid dehydrogenase: new insights into the conserved arginines for NADP(H) recognition.,Lou D, Wang B, Tan J, Zhu L, Cen X, Ji Q, Wang Y Sci Rep. 2016 Mar 10;6:22885. doi: 10.1038/srep22885. PMID:26961171<ref>PMID:26961171</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5epo" style="background-color:#fffaf0;"></div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Lou, D]] | [[Category: Lou, D]] | ||
| + | [[Category: Wang, B]] | ||
[[Category: Wang, F]] | [[Category: Wang, F]] | ||
| + | [[Category: Metabolic process]] | ||
| + | [[Category: Oxidoreductase]] | ||
| + | [[Category: Oxidoreductase activity]] | ||
Revision as of 13:03, 11 May 2016
The three-dimensional structure of Clostridium absonum 7alpha-hydroxysteroid dehydrogenase
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