Resolvase
From Proteopedia
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**[[1hcr]], [[1ijw]], [[1ijj6]], [[1jj8]], [[1jko]], [[1jkp]], [[1jkq]], [[1jkr]] – HRec + DNA – synthetic <br /> | **[[1hcr]], [[1ijw]], [[1ijj6]], [[1jj8]], [[1jko]], [[1jkp]], [[1jkq]], [[1jkr]] – HRec + DNA – synthetic <br /> | ||
| + | |||
| + | *RadA recombinase | ||
| + | |||
| + | **[[2zub]], [[2zuc]], [[2zud]], [[2dfl]] – SsRadA <br /> | ||
| + | **[[4dc9]] – MvRadA C-terminal domain <br /> | ||
| + | **[[2i1q]] – MvRadA (mutant) <br /> | ||
| + | **[[1pzn]] – PfRadA <br /> | ||
| + | **[[4a6p]] – PfRadA ATPase domain (mutant) <br /> | ||
| + | |||
| + | *RadA recombinase complex | ||
| + | |||
| + | **[[5fot]], [[5fou]], [[5fov]], [[5fow]], [[5fpk]], [[4b3b]] – PfRadA ATPase domain + tetrapeptide <br /> | ||
| + | **[[4d6p]] – PfRadA ATPase domain (mutant) + AMPPNP <br /> | ||
| + | **[[4uqo]] – PfRadA ATPase domain (mutant) + ADP <br /> | ||
| + | **[[4b2i]] – PfRadA ATPase domain (mutant) + indazole <br /> | ||
| + | **[[4b2l]], [[4b35]] – PfRadA ATPase domain (mutant) + methylester tryplophan <br /> | ||
| + | **[[4b32]], [[4b33]] – PfRadA ATPase domain (mutant) + naphthol <br /> | ||
| + | **[[4b34]] – PfRadA ATPase domain (mutant) + aminobenzothiazole <br /> | ||
| + | **[[4b3c]], [[4b3d]] – PfRadA ATPase domain (mutant) + indole <br /> | ||
| + | **[[4a6x]] – PfRadA ATPase domain (mutant) + ATP <br /> | ||
| + | **[[3ntu]], [[2gdj]] – MvRadA + AMPPNP <br /> | ||
| + | **[[2b21]], [[2f1h]], [[2f1i]], [[2fpl]], [[2fpm]] – MvRadA (mutant) + AMPPNP <br /> | ||
| + | **[[3fyh]], [[2f1j]], [[2fpk]] – MvRadA (mutant) + ADP <br /> | ||
| + | **[[3etl]], [[3ew9]], [[3ewa]] – MmRadA (mutant) + AMPPNP <br /> | ||
| + | |||
| + | *RadB recombinase | ||
| + | |||
| + | **[[2cvf]], [[2cvh]] – TkRadB <br /> | ||
* Integrase | * Integrase | ||
Revision as of 08:55, 4 August 2016
Function
Resolvase or recombinase (Rec) is a nuclease which is involved in DNA recombination. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase.
- Holliday junction resolvase (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands[1].
- Tyr-recombinases include Cre which recombines loxP sites, FLP and lambda integrase (LamInt).
- Ser-recombinases (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase.
- RadA recombinase promotes DNA recombination. Detailed analysis of its suggests mechanisms for junction isomerization and communication between the two active sites [2].
- Hin-recombinase (HRec) is a protein of Salmonella. HRec inverts a 900 base pair DNA segment which contains the promoters of flagellar genes. The inversion changes the these genes' expressions[3].
3D structures of resolvase
Updated on 04-August-2016
References
- ↑ West SC. The search for a human Holliday junction resolvase. Biochem Soc Trans. 2009 Jun;37(Pt 3):519-26. doi: 10.1042/BST0370519. PMID:19442245 doi:http://dx.doi.org/10.1042/BST0370519
- ↑ Haldenby S, White MF, Allers T. RecA family proteins in archaea: RadA and its cousins. Biochem Soc Trans. 2009 Feb;37(Pt 1):102-7. doi: 10.1042/BST0370102. PMID:19143611 doi:http://dx.doi.org/10.1042/BST0370102
- ↑ Dhar G, Sanders ER, Johnson RC. Architecture of the hin synaptic complex during recombination: the recombinase subunits translocate with the DNA strands. Cell. 2004 Oct 1;119(1):33-45. PMID:15454079 doi:http://dx.doi.org/10.1016/j.cell.2004.09.010
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