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5j31
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of 14-3-3zeta in complex with an alkyne cross-linked cyclic peptide derived from ExoS== | |
| + | <StructureSection load='5j31' size='340' side='right' caption='[[5j31]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5j31]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J31 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J31 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEZ:BENZOIC+ACID'>BEZ</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MK8:2-METHYL-L-NORLEUCINE'>MK8</scene>, <scene name='pdbligand=ZS8:(2S)-2-AMINO-2-METHYLDEC-8-YNOIC+ACID'>ZS8</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4n7g|4n7g]], [[4n7y|4n7y]], [[4n84|4n84]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j31 OCA], [http://pdbe.org/5j31 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j31 RCSB], [http://www.ebi.ac.uk/pdbsum/5j31 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j31 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/1433Z_HUMAN 1433Z_HUMAN]] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.<ref>PMID:9360956</ref> <ref>PMID:14578935</ref> <ref>PMID:15071501</ref> <ref>PMID:15644438</ref> <ref>PMID:16376338</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Macrocyclization can be used to constrain peptides in their bioactive conformations, thereby supporting target affinity and bioactivity. In particular, for the targeting of challenging protein-protein interactions, macrocyclic peptides have proven to be very useful. Available approaches focus on the stabilization of alpha-helices, which limits their general applicability. Here we report for the first time on the use of ring-closing alkyne metathesis for the stabilization of an irregular peptide secondary structure. A small library of alkyne-crosslinked peptides provided a number of derivatives with improved target affinity relative to the linear parent peptide. In addition, we report the crystal structure of the highest-affinity derivative in a complex with its protein target 14-3-3zeta. It can be expected that the alkyne-based macrocyclization of irregular binding epitopes should give rise to new scaffolds suitable for targeting of currently intractable proteins. | ||
| - | + | Constraining an Irregular Peptide Secondary Structure through Ring-Closing Alkyne Metathesis.,Cromm PM, Wallraven K, Glas A, Bier D, Furstner A, Ottmann C, Grossmann TN Chembiochem. 2016 Sep 6. doi: 10.1002/cbic.201600362. PMID:27596722<ref>PMID:27596722</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5j31" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bier, D]] | ||
| + | [[Category: Cromm, P]] | ||
| + | [[Category: Glas, A]] | ||
| + | [[Category: Grossmann, T]] | ||
| + | [[Category: Wallraven, K]] | ||
| + | [[Category: 14-3-3 protein zeta]] | ||
| + | [[Category: Alkyne cross-link]] | ||
| + | [[Category: Constrained peptide]] | ||
| + | [[Category: Protein-protein-interaction]] | ||
| + | [[Category: Signaling protein]] | ||
Revision as of 17:28, 19 October 2016
Crystal structure of 14-3-3zeta in complex with an alkyne cross-linked cyclic peptide derived from ExoS
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