OBrochtasSandbox
From Proteopedia
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This shows the basic structure of the kinase domain with an <scene name='75/750300/Kinase_n_and_c_lobes/1'>N and C lobe</scene>. The ATP will sit between them just below Beta 1 | This shows the basic structure of the kinase domain with an <scene name='75/750300/Kinase_n_and_c_lobes/1'>N and C lobe</scene>. The ATP will sit between them just below Beta 1 | ||
| - | <scene name='75/750300/Inr_atp-cat_loop/ | + | <scene name='75/750300/Inr_atp-cat_loop/3'>This</scene> shows the ATP binding site (purple) and the catalytic loop (green). |
Here it is as a <scene name='75/750300/Inr_atp-cat_loopspacefill/1'>spacefilled model</scene>. | Here it is as a <scene name='75/750300/Inr_atp-cat_loopspacefill/1'>spacefilled model</scene>. | ||
Revision as of 19:45, 16 January 2017
Anopheles arabiensis Insulin Receptor like Protein
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The Anopheles arabiensis Insulin Receptor's kinase domain was modeled by Brian Pierce for the 'An arab ts lethal project'
This shows the basic structure of the kinase domain with an . The ATP will sit between them just below Beta 1
shows the ATP binding site (purple) and the catalytic loop (green). Here it is as a .
These are found in other RTKs.
G1000S (magenta), A1133G (orange), T1221I (teal)
And as a
This when mutated to isoleucine resulted in ts lethal mutations in the sevenless and EGFR-like genes in D. melanogster
Using the software TS_pred (a ts mutation prediction program): these are the to mutate based on sequence and structure - L1236, I1071, V1196, L1197,I1258 (red) Using Rosetta_ts_rbf (another ts mutation prediction program): these are the sites -W1173P, M1075K, Y1208G, L1197P, Y1208Asn (yellow)
Both TS_pred and Rosetta_ts_rbf had in their top 5 (black)
Most but not all of the actual and predicted mutations are . Note that T1221 and G1000S are not buried.
Here are the .
