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1qxp

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Revision as of 11:17, 7 October 2017

Crystal Structure of a mu-like calpain

Structural highlights

1qxp is a 2 chain structure with sequence from Buffalo rat. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1df0, 1dkv
Activity:	Calpain-1, with EC number 3.4.22.52
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CAN2_RAT] Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The two Ca2+-dependent cysteine proteases, micro- and m-calpain, are involved in various Ca2+-linked signal pathways but differ markedly in their Ca2+ requirements for activation. We have determined the structure of a micro-like calpain, which has 85% micro-calpain sequence (the first 48 and the last 62 residues of the large subunit are those from m-calpain) and a low Ca2+ requirement. This construct was used because micro-calpain itself is too poorly expressed. The structure of micro-like calpain is very similar in overall fold to that of m-calpain as expected, but differs significantly in two aspects. In comparison with m-calpain, the catalytic triad residues in micro-like calpain, His and Cys, are much closer together in the absence of Ca2+, and significant portions of the Ca2+ binding EF-hand motifs are disordered and more flexible. These structural differences imply that Ca2+-free micro-calpain may represent a partially activated structure, requiring lower Ca2+ concentration to trigger its activation.

Crystal structure of a micro-like calpain reveals a partially activated conformation with low Ca2+ requirement.,Pal GP, De Veyra T, Elce JS, Jia Z Structure. 2003 Dec;11(12):1521-6. PMID:14656436^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Pal GP, De Veyra T, Elce JS, Jia Z. Crystal structure of a micro-like calpain reveals a partially activated conformation with low Ca2+ requirement. Structure. 2003 Dec;11(12):1521-6. PMID:14656436

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1qxp"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of a mu-like calpain==
 <StructureSection load='1qxp' size='340' side='right' caption='[[1qxp]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1df0|1df0]], [[1dkv|1dkv]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Calpain-1 Calpain-1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.52 3.4.22.52] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qxp OCA], [http://pdbe.org/1qxp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qxp RCSB], [http://www.ebi.ac.uk/pdbsum/1qxp PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qxp OCA], [http://pdbe.org/1qxp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qxp RCSB], [http://www.ebi.ac.uk/pdbsum/1qxp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1qxp ProSAT]</span></td></tr>
 </table>
 == Function ==

1qxp

From Proteopedia

Revision as of 11:17, 7 October 2017

Crystal Structure of a mu-like calpain

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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