Sandbox Reserved 1476

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(Difference between revisions)

Revision as of 18:13, 3 December 2018

This Sandbox is Reserved from November 5 2018 through January 1, 2019 for use in the course "CHEM 4923: Senior Project taught by Christina R. Bourne at the University of Oklahoma, Norman, USA. This reservation includes Sandbox Reserved 1471 through Sandbox Reserved 1478.

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PDB ID 3AIE

This enzyme is a glucansucrase from Streptococcus mutans.^[1] These enzymes are large and extracellularly expressed by few other bacterial species.^[2] Their main function is to catalyze the production of glucose polymers from sucrose substrates.^[1] These polymers comprise the dental plaque that promotes tooth decay in humans. For this reason, knowledge of the structure, genome, and proteome of this enzyme is critical for the development of an inhibitor in an effort to reduce the prevalence of cavities and periodontal disease.

1 Structure
- 1.1 Catalytic Domain
- 1.2 Supersecondary Structure
2 Function
3 Disease
4 Relevance
5 References

Structure

The structure at right can be organized into regions and domains. There are three main regions: N-terminal region, catalytic region, and C-terminal glucan binding region.^[1] The domains are more complex. There are five total domains; however, only four are visible in this crystal structure. Each domain consists of an N-terminal and C-terminal portion of the polypeptide chain, except Domain C, which is located at the bottom of the enzyme.^[1] Following the polypeptide chain from N-terminus to C-terminus would pass through the domains in the following order: V, IV, B, A, C, A, B, IV, V.^[2] CAN I ADD THE IMAGE FROM LEEMHUIS COMPARISON? A high degree of structural similarity exists within the GH70 family enzymes.^[2]

Catalytic Domain

Domain A is regarded as the catalytic domain.^[1] Within it, exists a highly conserved , which includes two aspartic acid residues and a general acid/base glutamate.^[1] A calcium ion is also bound to help stabilize the formation of the catalytic domain.^[1]

Supersecondary Structure

This enzyme features two well-known motifs. Domain A consists of a TIM barrel.^[1] It is composed of a ring of beta strands surrounded by a ring of alpha helices. Domain C contains a greek key motif, which is four antiparallel beta strands that form a sheet.^[2]

Function

Disease

Relevance

Based on the enzyme's contributions to dental cavity formation, this enzyme and its genes are attractive targets for inhibition.

References

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Ito K, Ito S, Shimamura T, Weyand S, Kawarasaki Y, Misaka T, Abe K, Kobayashi T, Cameron AD, Iwata S. Crystal Structure of Glucansucrase from the Dental Caries Pathogen Streptococcus mutans. J Mol Biol. 2011 Feb 25. PMID:21354427 doi:10.1016/j.jmb.2011.02.028
↑ ^2.0 ^2.1 ^2.2 ^2.3 Leemhuis H, Pijning T, Dobruchowska JM, van Leeuwen SS, Kralj S, Dijkstra BW, Dijkhuizen L. Glucansucrases: three-dimensional structures, reactions, mechanism, alpha-glucan analysis and their implications in biotechnology and food applications. J Biotechnol. 2013 Jan 20;163(2):250-72. doi: 10.1016/j.jbiotec.2012.06.037. Epub, 2012 Jul 10. PMID:22796091 doi:http://dx.doi.org/10.1016/j.jbiotec.2012.06.037

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 ==PDB ID 3AIE==
-This enzyme is a glucansucrase from ''Streptococcus mutans''.<ref name="Ito">PMID:21354427</ref>. These enzymes are large and extracellularly expressed by few other bacterial species.<ref name="Leemhuis">PMID:22796091</ref>. Their main function is to catalyze the production of glucose polymers from sucrose substrates.<ref name="Ito" />. These polymers comprise the dental plaque that promotes tooth decay in humans. For this reason, knowledge of the structure, genome, and proteome of this enzyme is critical for the development of an inhibitor in an effort to reduce the prevalence of cavities and periodontal disease.
+This enzyme is a glucansucrase from ''Streptococcus mutans''.<ref name="Ito">PMID:21354427</ref> These enzymes are large and extracellularly expressed by few other bacterial species.<ref name="Leemhuis">PMID:22796091</ref> Their main function is to catalyze the production of glucose polymers from sucrose substrates.<ref name="Ito" /> These polymers comprise the dental plaque that promotes tooth decay in humans. For this reason, knowledge of the structure, genome, and proteome of this enzyme is critical for the development of an inhibitor in an effort to reduce the prevalence of cavities and periodontal disease.
 <StructureSection load='3aie' size='350' side='right' caption='GTF-SI of Streptococcus mutans (PDB entry [[3aie]])' scene=''>

Sandbox Reserved 1476

From Proteopedia

Revision as of 18:13, 3 December 2018

PDB ID 3AIE

Contents

Structure

Catalytic Domain

Supersecondary Structure

Function

Disease

Relevance

References

Views

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