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6s6x
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of Azospirillum brasilense Glutamate Synthase in a6b6 oligomeric state.== | |
| + | <StructureSection load='6s6x' size='340' side='right'caption='[[6s6x]], [[Resolution|resolution]] 3.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6s6x]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29145 Atcc 29145]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S6X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6S6X FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gltB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=192 ATCC 29145]), gltD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=192 ATCC 29145])</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate_synthase_(NADPH) Glutamate synthase (NADPH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.1.13 1.4.1.13] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6s6x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s6x OCA], [http://pdbe.org/6s6x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6s6x RCSB], [http://www.ebi.ac.uk/pdbsum/6s6x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6s6x ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bacterial NADPH-dependent glutamate synthase (GltS) is a complex iron-sulfur flavoprotein that catalyzes the reductive synthesis of two L-Glu molecules from L-Gln and 2-oxo-glutarate. GltS functional unit hosts an alpha-subunit (alphaGltS) and a beta-subunit (betaGltS) that assemble in different alphabeta oligomers in solution. Here, we present the cryo-electron microscopy structures of Azospirillum brasilense GltS in four different oligomeric states (alpha4beta3, alpha4beta4, alpha6beta4 and alpha6beta6, in the 3.5- to 4.1-A resolution range). Our study provides a comprehensive GltS model that details the inter-protomeric assemblies and allows unequivocal location of the FAD cofactor and of two electron transfer [4Fe-4S](+1,+2) clusters within betaGltS. | ||
| - | + | Cryo-EM Structures of Azospirillum brasilense Glutamate Synthase in Its Oligomeric Assemblies.,Swuec P, Chaves-Sanjuan A, Camilloni C, Vanoni MA, Bolognesi M J Mol Biol. 2019 Aug 29. pii: S0022-2836(19)30520-0. doi:, 10.1016/j.jmb.2019.08.011. PMID:31473159<ref>PMID:31473159</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6s6x" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Atcc 29145]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Bolognesi, M]] | ||
| + | [[Category: Camilloni, C]] | ||
| + | [[Category: Chaves-Sanjuan, A]] | ||
| + | [[Category: Complex]] | ||
| + | [[Category: Flavoprotein]] | ||
| + | [[Category: Glutamate synthesy]] | ||
| + | [[Category: Oligomeric assembly]] | ||
Revision as of 09:43, 30 October 2019
Structure of Azospirillum brasilense Glutamate Synthase in a6b6 oligomeric state.
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