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5ayd

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Revision as of 09:55, 26 February 2020

Crystal structure of Ruminococcus albus beta-(1,4)-mannooligosaccharide phosphorylase (RaMP2) in complexes with phosphate

Structural highlights

5ayd is a 6 chain structure with sequence from Ruma7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	5aye, 5ayc, 5ay9
Gene:	Rumal_0099 (RUMA7)
Activity:	Beta-1,4-mannooligosaccharide phosphorylase, with EC number 2.4.1.319
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MOSP_RUMA7] Catalyzes the phosphorolysis of beta-1,4-mannooligosaccharides to mannose 1-phosphate (Man1P) and shorter mannooligosaccharides. Can also catalyze the phosphorolysis of 4-O-beta-D-mannopyranosyl-D-glucopyranose (Man-Glc), but shows higher activity toward longer mannooligosaccharides. Involved in a mannan catabolic pathway which feeds into glycolysis.^[1]

Publication Abstract from PubMed

In Ruminococcus albus, 4-O-beta-d-mannosyl-d-glucose phosphorylase (RaMP1) and beta-(1,4)-mannooligosaccharide phosphorylase (RaMP2) belong to two subfamilies of glycoside hydrolase family 130. The two enzymes phosphorolyze beta-mannosidic linkages at the nonreducing ends of their substrates, and have substantially diverse substrate specificity. The differences in their mechanism of substrate binding have not yet been fully clarified. In the present study, we report the crystal structures of RaMP1 with/without 4-O-beta-d-mannosyl-d-glucose and RaMP2 with/without beta-(1-->4)-mannobiose. The structures of the two enzymes differ at the +1 subsite of the substrate-binding pocket. Three loops are proposed to determine the different substrate specificities. One of these loops is contributed from the adjacent molecule of the oligomer structure. In RaMP1, His245 of loop 3 forms a hydrogen-bond network with the substrate through a water molecule, and is indispensible for substrate binding.

Structural insights into the difference in substrate recognition of two mannoside phosphorylases from two GH130 subfamilies.,Ye Y, Saburi W, Odaka R, Kato K, Sakurai N, Komoda K, Nishimoto M, Kitaoka M, Mori H, Yao M FEBS Lett. 2016 Mar;590(6):828-37. doi: 10.1002/1873-3468.12105. Epub 2016 Mar 4. PMID:26913570^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kawahara R, Saburi W, Odaka R, Taguchi H, Ito S, Mori H, Matsui H. Metabolic mechanism of mannan in a ruminal bacterium, Ruminococcus albus, involving two mannoside phosphorylases and cellobiose 2-epimerase: discovery of a new carbohydrate phosphorylase, beta-1,4-mannooligosaccharide phosphorylase. J Biol Chem. 2012 Dec 7;287(50):42389-99. doi: 10.1074/jbc.M112.390336. Epub 2012, Oct 23. PMID:23093406 doi:http://dx.doi.org/10.1074/jbc.M112.390336
↑ Ye Y, Saburi W, Odaka R, Kato K, Sakurai N, Komoda K, Nishimoto M, Kitaoka M, Mori H, Yao M. Structural insights into the difference in substrate recognition of two mannoside phosphorylases from two GH130 subfamilies. FEBS Lett. 2016 Mar;590(6):828-37. doi: 10.1002/1873-3468.12105. Epub 2016 Mar 4. PMID:26913570 doi:http://dx.doi.org/10.1002/1873-3468.12105

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5ayd"

@@ Line 1: / Line 1: @@
 ==Crystal structure of Ruminococcus albus beta-(1,4)-mannooligosaccharide phosphorylase (RaMP2) in complexes with phosphate==
-<StructureSection load='5ayd' size='340' side='right' caption='[[5ayd]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+<StructureSection load='5ayd' size='340' side='right'caption='[[5ayd]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5ayd]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AYD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AYD FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5ayd]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Ruma7 Ruma7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AYD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AYD FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5aye|5aye]], [[5ayc|5ayc]], [[5ay9|5ay9]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Rumal_0099 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=697329 RUMA7])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-1,4-mannooligosaccharide_phosphorylase Beta-1,4-mannooligosaccharide phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.319 2.4.1.319] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ayd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ayd OCA], [http://pdbe.org/5ayd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ayd RCSB], [http://www.ebi.ac.uk/pdbsum/5ayd PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ayd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ayd OCA], [http://pdbe.org/5ayd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ayd RCSB], [http://www.ebi.ac.uk/pdbsum/5ayd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ayd ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 25: / Line 26: @@
 </StructureSection>
 [[Category: Beta-1,4-mannooligosaccharide phosphorylase]]
+[[Category: Large Structures]]
+[[Category: Ruma7]]
 [[Category: Kato, K]]
 [[Category: Saburi, W]]

5ayd

From Proteopedia

Revision as of 09:55, 26 February 2020

Crystal structure of Ruminococcus albus beta-(1,4)-mannooligosaccharide phosphorylase (RaMP2) in complexes with phosphate

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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