6lng
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Rapid crystallization of streptavidin using charged peptides== | |
| + | <StructureSection load='6lng' size='340' side='right'caption='[[6lng]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6lng]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LNG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6LNG FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6lng FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lng OCA], [http://pdbe.org/6lng PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lng RCSB], [http://www.ebi.ac.uk/pdbsum/6lng PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lng ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We utilized electrostatic interaction to induce rapid crystallization of streptavidin. Simply mixing streptavidins possessing either a positively or negatively charged peptide at their C-terminus generated diffraction-quality crystals in a few hours. We modified the streptavidin crystals with fluorescent molecules using biotin, demonstrating the concept of protein crystals as functional biomaterials. | ||
| - | + | Genetically fused charged peptides induce rapid crystallization of proteins.,Minamihata K, Tsukamoto K, Adachi M, Shimizu R, Mishina M, Kuroki R, Nagamune T Chem Commun (Camb). 2020 Mar 5. doi: 10.1039/c9cc09529b. PMID:32134050<ref>PMID:32134050</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 6lng" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | [[Category: | + | </StructureSection> |
| + | [[Category: Large Structures]] | ||
[[Category: Adachi, M]] | [[Category: Adachi, M]] | ||
| + | [[Category: Kuroki, R]] | ||
| + | [[Category: Minamihata, K]] | ||
| + | [[Category: Mishina, M]] | ||
[[Category: Nagamune, T]] | [[Category: Nagamune, T]] | ||
| + | [[Category: Shimizu, R]] | ||
| + | [[Category: Tsukamoto, K]] | ||
| + | [[Category: Biotin-binding protein]] | ||
| + | [[Category: Peptide binding protein]] | ||
Revision as of 09:41, 18 March 2020
Rapid crystallization of streptavidin using charged peptides
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