Sandbox UC 9
From Proteopedia
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| + | <Structure load='' size='400' frame='true' align='right' caption='Candida rugosa lipase ([[1trh]], [[1lpm]]).' scene='Lipase_lid_morph/Lightseagreen_hinge/1' /> | ||
| + | For an introduction to the structure and function of lipase, please see the article [[Lipase]]. This ''Lipase lid morph'' article is a supplement to the main article on [[Lipase]]. | ||
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| + | ''Candida rugosa'' lipase (triacylglycerol hydrolase) has been observed in two conformations, with the "lid" (residues 66-92<ref name='2states1994' />) closed ([[1trh]]) or open ([[1lpm]])<ref name='2states1994'>PMID: 8142901</ref>. | ||
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| + | *Closed <font color='lightseagreen'><b>LID</b></font> (<scene name='Lipase_lid_morph/Lightseagreen_hinge/1'>restore initial scene</scene>). | ||
| + | *<scene name='Lipase_lid_morph/Lightseagreen_hinge/4'>Open</scene> <font color='lightseagreen'><b>LID</b></font> with inhibitor (1R)-menthyl hexyl phosphonate (<b><font color='#909090'>C</font> <font color='#ff0d0d'>O</font> <font color='#ff8000'>P</font></b>). | ||
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| + | A morph<ref>This is a [[Morphs#Linear_Interpolation|linear interpolation morph]]. The 14-model PDB file is [[Image:Morph-linear-1trh-1lpm.pdb.gz]].</ref> shows the lid opening and closing. | ||
| + | *<scene name='Lipase_lid_morph/Lightseagreen_hinge/2'>Cartoon morph</scene> (<font color='lightseagreen'><b>LID</b></font>). | ||
| + | *<scene name='Lipase_lid_morph/Lightseagreen_hinge/3'>Spacefilling morph</scene> (<font color='lightseagreen'><b>LID</b></font>, <font color='orange'><b>catalytic triad: Ser209, Glu341, and His449</b></font><ref name='2states1994' />).<br> | ||
| + | {{Template:Button Toggle Animation2}} | ||
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| + | When the lid is closed, the enzyme surface is largely {{Template:ColorKey_Polar}}. When the lid opens, a {{Template:ColorKey_Hydrophobic}} pocket is exposed with the catalytic triad in the bottom. | ||
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| + | *<scene name='Lipase_lid_morph/Lightseagreen_hinge/5'>Spacefilling morph</scene> ({{Template:ColorKey_Polar}}, {{Template:ColorKey_Hydrophobic}}, <font color='orange'><b>catalytic triad: Ser209, Glu341, and His449</b></font><ref name='2states1994' />). | ||
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| + | ==See Also== | ||
| + | *[[Lipase]], the main article in Proteopedia. | ||
| + | *[[Molecular Playground/Pancreatic Lipase]] | ||
| + | *[http://en.wikipedia.org/wiki/Lipase Lipase in Wikipedia] | ||
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| + | ==Notes and References== | ||
| + | <references /> | ||
Revision as of 19:22, 8 May 2020
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Contents |
Title
subtitle
For an introduction to the structure and function of lipase, please see the article Lipase. This Lipase lid morph article is a supplement to the main article on Lipase.
Candida rugosa lipase (triacylglycerol hydrolase) has been observed in two conformations, with the "lid" (residues 66-92[1]) closed (1trh) or open (1lpm)[1].
- Closed LID ().
- LID with inhibitor (1R)-menthyl hexyl phosphonate (C O P).
A morph[2] shows the lid opening and closing.
- (LID).
- (LID, catalytic triad: Ser209, Glu341, and His449[1]).
When the lid is closed, the enzyme surface is largely Polar. When the lid opens, a Hydrophobic pocket is exposed with the catalytic triad in the bottom.
- (Polar, Hydrophobic, catalytic triad: Ser209, Glu341, and His449[1]).
See Also
- Lipase, the main article in Proteopedia.
- Molecular Playground/Pancreatic Lipase
- Lipase in Wikipedia
Notes and References
- ↑ 1.0 1.1 1.2 1.3 Grochulski P, Li Y, Schrag JD, Cygler M. Two conformational states of Candida rugosa lipase. Protein Sci. 1994 Jan;3(1):82-91. PMID:8142901
- ↑ This is a linear interpolation morph. The 14-model PDB file is Image:Morph-linear-1trh-1lpm.pdb.gz.
