2r5v
From Proteopedia
(Difference between revisions)
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==Hydroxymandelate Synthase Crystal Structure== | ==Hydroxymandelate Synthase Crystal Structure== | ||
| - | <StructureSection load='2r5v' size='340' side='right' caption='[[2r5v]], [[Resolution|resolution]] 2.30Å' scene=''> | + | <StructureSection load='2r5v' size='340' side='right'caption='[[2r5v]], [[Resolution|resolution]] 2.30Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2r5v]] is a 2 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[2r5v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"streptomyces_orientalis"_pittenger_and_brigham_1956 "streptomyces orientalis" pittenger and brigham 1956]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R5V FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=HHH:(2S)-HYDROXY(4-HYDROXYPHENYL)ETHANOIC+ACID'>HHH</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=HHH:(2S)-HYDROXY(4-HYDROXYPHENYL)ETHANOIC+ACID'>HHH</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HmaS ([ | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HmaS ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=31958 "Streptomyces orientalis" Pittenger and Brigham 1956])</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/4-hydroxymandelate_synthase 4-hydroxymandelate synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.46 1.13.11.46] </span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r5v OCA], [https://pdbe.org/2r5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r5v RCSB], [https://www.ebi.ac.uk/pdbsum/2r5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r5v ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [[ | + | [[https://www.uniprot.org/uniprot/HMAS_AMYOR HMAS_AMYOR]] Required to synthesize hydroxyphenylglycine, a recurring skeletal component of nonproteinogenic macrocyclic peptide antibiotics such as vancomycin. Catalyzes the conversion of p-hydroxyphenylpyruvate to p-hydroxymandelate. The decarboxylation and hydroxylation activities of HmaS show novel and distinct regioselectivity, compared to all other known p-hydroxyphenylpyruvate dioxygenases, by hydroxylating the benzylic position of the substrate instead of the phenyl ring.<ref>PMID:11137816</ref> |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r5/2r5v_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r5/2r5v_consurf.spt"</scriptWhenChecked> |
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
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[[Category: Streptomyces orientalis pittenger and brigham 1956]] | [[Category: Streptomyces orientalis pittenger and brigham 1956]] | ||
[[Category: 4-hydroxymandelate synthase]] | [[Category: 4-hydroxymandelate synthase]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Brownlee, J M]] | [[Category: Brownlee, J M]] | ||
[[Category: Harrison, D H.T]] | [[Category: Harrison, D H.T]] | ||
Revision as of 04:15, 2 July 2021
Hydroxymandelate Synthase Crystal Structure
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