Methionine synthase
From Proteopedia
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PDB ID: 1K7Y - cobalamin, 1K98 - AdoMet complex | PDB ID: 1K7Y - cobalamin, 1K98 - AdoMet complex | ||
| - | The full structure of <scene name='90/907471/Superposition_1/2'>methionine synthase</scene> has yet to be determined but we understand it contains 4 domains for its catalytic | + | The full structure of <scene name='90/907471/Superposition_1/2'>methionine synthase</scene> has yet to be determined but we understand it contains 4 domains for its catalytic and reactivation cycles consisting of homocysteine, B12, THF, and SAH (as part of the SAM cycle).<ref>DOI: 10.1038/nsb738</ref> |
</StructureSection> | </StructureSection> | ||
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== Vitamin B-12 == | == Vitamin B-12 == | ||
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== Oxidation States of Cobalamin == | == Oxidation States of Cobalamin == | ||
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This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | ||
| - | </StructureSection> | ||
== References == | == References == | ||
<ref>DOI: 10.1128/JB.00208-06</ref> | <ref>DOI: 10.1128/JB.00208-06</ref> | ||
<references/> | <references/> | ||
Revision as of 18:05, 5 April 2022
Contents |
Methionine synthase
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The change from homocysteine to methionine is an SN2 reaction where the methyl group from methyltetrahydrofolate (MTHF), located on N-5, is donated. MTHF is a product of MTHFR.
This is a complex reaction as the product, tetrahydrofolate, is a poor leaving group, thus requiring a "supernucleophile" with a protein-bound B-12 vitamin Cobalamin as the methyl carrier.
Vitamin B-12
Oxidation States of Cobalamin
Relevance
Structural highlights
This is a sample scene created with SAT to by Group, and another to make of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.
References
- ↑ Kung Y, Ando N, Doukov TI, Blasiak LC, Bender G, Seravalli J, Ragsdale SW, Drennan CL. Visualizing molecular juggling within a B(12)-dependent methyltransferase complex. Nature. 2012 Mar 14. doi: 10.1038/nature10916. PMID:22419154 doi:10.1038/nature10916
- ↑ Bandarian V, Pattridge KA, Lennon BW, Huddler DP, Matthews RG, Ludwig ML. Domain alternation switches B(12)-dependent methionine synthase to the activation conformation. Nat Struct Biol. 2002 Jan;9(1):53-6. PMID:11731805 doi:10.1038/nsb738
- ↑ Barra L, Fontenelle C, Ermel G, Trautwetter A, Walker GC, Blanco C. Interrelations between glycine betaine catabolism and methionine biosynthesis in Sinorhizobium meliloti strain 102F34. J Bacteriol. 2006 Oct;188(20):7195-204. doi: 10.1128/JB.00208-06. PMID:17015658 doi:http://dx.doi.org/10.1128/JB.00208-06
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