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| | ==Structural and Functional Characterization of the Yeast Mg2+ Channel Mrs2== | | ==Structural and Functional Characterization of the Yeast Mg2+ Channel Mrs2== |
| - | <StructureSection load='3rkg' size='340' side='right' caption='[[3rkg]], [[Resolution|resolution]] 1.28Å' scene=''> | + | <StructureSection load='3rkg' size='340' side='right'caption='[[3rkg]], [[Resolution|resolution]] 1.28Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3rkg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RKG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RKG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3rkg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RKG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RKG FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MRS2, YOR334W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr> | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MRS2, YOR334W ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rkg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rkg OCA], [http://pdbe.org/3rkg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rkg RCSB], [http://www.ebi.ac.uk/pdbsum/3rkg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rkg ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rkg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rkg OCA], [https://pdbe.org/3rkg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rkg RCSB], [https://www.ebi.ac.uk/pdbsum/3rkg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rkg ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MRS2_YEAST MRS2_YEAST]] High-conductance magnesium-selective channel that mediates the influx of magnesium into the mitochondrial matrix. Essential for the splicing of mRNA group II introns in mitochondria by affecting mitochondrial magnesium concentrations, which are critical for group II intron splicing. It also suppresses a variety of mitochondrial intron mutations and its absence may disturb the assembly of mitochondrial membrane complexes.<ref>PMID:11544180</ref> <ref>PMID:12628916</ref> <ref>PMID:17827224</ref> <ref>PMID:20653776</ref> | + | [[https://www.uniprot.org/uniprot/MRS2_YEAST MRS2_YEAST]] High-conductance magnesium-selective channel that mediates the influx of magnesium into the mitochondrial matrix. Essential for the splicing of mRNA group II introns in mitochondria by affecting mitochondrial magnesium concentrations, which are critical for group II intron splicing. It also suppresses a variety of mitochondrial intron mutations and its absence may disturb the assembly of mitochondrial membrane complexes.<ref>PMID:11544180</ref> <ref>PMID:12628916</ref> <ref>PMID:17827224</ref> <ref>PMID:20653776</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Atcc 18824]] | | [[Category: Atcc 18824]] |
| | + | [[Category: Large Structures]] |
| | [[Category: Carugo, O]] | | [[Category: Carugo, O]] |
| | [[Category: Djinovic-Carugo, K]] | | [[Category: Djinovic-Carugo, K]] |
| Structural highlights
Function
[MRS2_YEAST] High-conductance magnesium-selective channel that mediates the influx of magnesium into the mitochondrial matrix. Essential for the splicing of mRNA group II introns in mitochondria by affecting mitochondrial magnesium concentrations, which are critical for group II intron splicing. It also suppresses a variety of mitochondrial intron mutations and its absence may disturb the assembly of mitochondrial membrane complexes.[1] [2] [3] [4]
Publication Abstract from PubMed
Mg(2+) translocation across cellular membranes is crucial for a myriad of physiological processes. Eukaryotic Mrs2 transporters are distantly related to the major bacterial Mg(2+) transporter CorA, the structure of which displays a bundle of giant alpha-helices forming a long pore that extends beyond the membrane before widening into a funnel-shaped cytosolic domain. Here, a functional and structural analysis of the regulatory domain of the eukaryotic Mg(2+) channel Mrs2 from the yeast inner mitochondrial membrane is presented using crystallography, genetics, biochemistry and fluorescence spectroscopy. Surprisingly, the fold of the Mrs2 regulatory domain bears notable differences compared with the related bacterial channel CorA. Nevertheless, structural analysis showed that analogous residues form functionally critical sites, notably the hydrophobic gate and the Mg(2+)-sensing site. Validation of candidate residues was performed by functional studies of mutants in isolated yeast mitochondria. Measurements of the Mg(2+) influx into mitochondria confirmed the involvement of Met309 as the major gating residue in Mrs2, corresponding to Met291 in CorA.
Structural and functional characterization of the N-terminal domain of the yeast Mg(2+) channel Mrs2.,Khan MB, Sponder G, Sjoblom B, Svidova S, Schweyen RJ, Carugo O, Djinovic-Carugo K Acta Crystallogr D Biol Crystallogr. 2013 Sep 1;69(Pt 9):1653-64. doi:, 10.1107/S0907444913011712. Epub 2013 Aug 15. PMID:23999289[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gregan J, Kolisek M, Schweyen RJ. Mitochondrial Mg(2+) homeostasis is critical for group II intron splicing in vivo. Genes Dev. 2001 Sep 1;15(17):2229-37. PMID:11544180 doi:10.1101/gad.201301
- ↑ Kolisek M, Zsurka G, Samaj J, Weghuber J, Schweyen RJ, Schweigel M. Mrs2p is an essential component of the major electrophoretic Mg2+ influx system in mitochondria. EMBO J. 2003 Mar 17;22(6):1235-44. PMID:12628916 doi:10.1093/emboj/cdg122
- ↑ Schindl R, Weghuber J, Romanin C, Schweyen RJ. Mrs2p forms a high conductance Mg2+ selective channel in mitochondria. Biophys J. 2007 Dec 1;93(11):3872-83. Epub 2007 Sep 7. PMID:17827224 doi:10.1529/biophysj.107.112318
- ↑ Sponder G, Svidova S, Schindl R, Wieser S, Schweyen RJ, Romanin C, Froschauer EM, Weghuber J. Lpe10p modulates the activity of the Mrs2p-based yeast mitochondrial Mg2+ channel. FEBS J. 2010 Sep;277(17):3514-25. doi: 10.1111/j.1742-4658.2010.07761.x. Epub, 2010 Jul 23. PMID:20653776 doi:10.1111/j.1742-4658.2010.07761.x
- ↑ Khan MB, Sponder G, Sjoblom B, Svidova S, Schweyen RJ, Carugo O, Djinovic-Carugo K. Structural and functional characterization of the N-terminal domain of the yeast Mg(2+) channel Mrs2. Acta Crystallogr D Biol Crystallogr. 2013 Sep 1;69(Pt 9):1653-64. doi:, 10.1107/S0907444913011712. Epub 2013 Aug 15. PMID:23999289 doi:10.1107/S0907444913011712
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