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Introduction

Figure 1: An overview of the Thyroid System. A depiction of signaling cascade from the hypothalamus ending in the release of TSH causing T3 and T4 production and its effects. The mechanism of regulation also shown by negative feedback from the T3 and T4 hormones. Source: [1]

Thyroid Stimulating Hormone Receptor (TSHR) is a type of G-Protein Coupled Receptor (GPCR) found in human thyroid follicles. It is activated by the Thyroid Stimulating Hormone (TSH) which is known as thyrotropin. Activation of TSHR is neccessary for activating a signaling pathway for the production of thyroid hormones such as T₃ and T₄ (Fig 1).

Structure

TSHR forms a complex with TSH and G_s proteins. This is called the . : Leucine Rich Region Domain (coral), the hinge region (blue-purple), and the transmembrane region(rainbow). The leucine rich region domain is extracellular. This is where TSH will bind. The hinge region is also extracellular. Conformational changes in this region are responsible for the switch between the active vs inactive state. Finally, the transmembrane region is located within the plasma membrane. Its function is to hold the receptor into the membrane. This domain is also bound to the G-proteins at the N-terminus. The G-proteins are located on the intracellular side of the plasma membrane. They are important for transmitting the binding signal into the cell, setting off a protein signaling cascade.

Transmembrane Region

() is embedded within the cell membrane. Like other G-protein receptors, it is made up of a 7-pass helix ^[1]. It is made up of about 284 residues. The transmembrane region is surrounded by a "belt" of . When cholesterol binding sites are mutated such that they are unfunctional, TSHR activity decreases. Thus, the cholesterols are important for TSHR function ^[2]. Additionally, at the N-terminus, the transmembrane region binds to the , which are located intracellularly.

Leucine Rich Domain

The is part of the extracellular region of TSHR. It is made up of about 280 different residues. Connected to its C-terminus is the Hinge Region. It is made up of an extensive parallel β-sheet. This β-sheet is where TSH binds and is called the binding pocket^[2]. The is a concave structure with many polar residues. This pocket is where the TSH antibody and agonist K1 bind as well as the agonist M22. These structures interact with specific residues to result in a structural change of the molecule. There is a mutation done by N-glycans at asparagine residues that plays a large role in the binding of TSH. The negative charge on these glycans contributes to the polarity of the binding pocket which mediates the binding efficiency of TSH. It has been shown that four of the five N glycan sites must be glycosylated to be in the active form^[3].

Hinge Region

The (purple-blue) connects the Transmembrane Region to the Leucine Rich Domain. It is sometimes referred to as the signaling specificity domain because there is some evidence suggesting that this region is important in both TSH binding and signal transduction. ^[4]. It is made up of two α-helices that are connected via disulfide bonds(shown in yellow). Interactions between these two helices and TSH help orient TSH properly. These interactions are essential for TSH binding, however, they are not required for the activation of TSHR. Conformational changes in this region, specifically the orientation of , are responsible for bringing TSHR into the active state ^[1].

Antibodies

TSH

M22

K1

Active vs. Inactive State

Figure 2: An overview of the Inactive (red) vs Active (green) state of TSHR. PDB: 7WX5

Figure 3: A zoomed in view of the Y279 residue in the Hinge Region of TSHR, showing the 6 angstrom move of Y279 during the activation of TSHR. Active TSHR is shown in green (PDB: 7t9i) and inactive TSHR is shown in pink (PDB: 7t9m).

When TSHR is not bound to TSH, it is in the . This is also considered the "down" state because the LRRD is pointing down. When TSH binds to TSHR, steric clashing between TSH and the cell-membrane cause TSHR to take on the . During this transition, the Extracellular domains rotate 55° along an axis. This rotation is caused by conformational changes within the , specifically at the . This residue moves 6 angstroms relative to I486, which is a residue located in the Transmembrane Region ^[1]. The active form is found when . The structure can be seen as straight. The inactive form is found when of TSHR is found when bound with K1. The overall structure of the molecule is bent when K1 is bound.

Specific Residues and Interactions

There are two in the binding pocket of TSHR that are the main contributors to the binding of the antibodies. The concave structure of the binding pocket allows a with the antibodies. These antibodies make tight interactions with a lot of intermolecular forces at play. interacts with Glu 118 on the antibodies to make a salt bridge interaction. interacts with Asp 111 on the antibodies to make a salt bridge interaction. Specifically, the two residues make an ionic interaction. The interaction is not close enough to make a hydrogen bond. Instead, the interaction between the Lys residues with the Asp or Glu residues is a salt bridge interaction. This is the main bond that holds these two molecules together. When in the inactive form, LYS 209 does not interact with any residue but LYS 58 has interaction with Glu 118 and this interaction pulls the molecule into the bent position. The salt bridge interaction between Lys and Glu is very specific. Lys was mutated with Arg and was expected to make the same salt bride interaction with Glu, however it make a completely different interaction. This new interaction favors a gain of function towards hCG ^[5].

Biological Relevance

Figure 4: T3 and T4 role in TSH concentration: Highlighting the problem when under or overactive on the metabolism. When an antibody is bound to TSHR and cannot respond to the negative feedback look the metabolism experiences a shift outside of equilibrium resulting in a wide array of side effects. [2]

The thyroid plays an essential role in the body's metabolism. The body's metabolism affects things like heart rate, digestion, temperature regulation, and many more things. When TSH is bound to TSHR, a signal is sent to produce T3 and T4. T4 is considered an inactive form and needs to be converted into T3 to become active. Those hormones then go impact cells in your body to increase or decrease your metabolism. The T3 and T4 hormones use a feedback system to regulate the release of TSH. When the hormone levels are high they travel through the bloodstream to prevent TSH production. When the hormones are low they are not able to continue the negative feedback loop, thus allowing TSH to be released to bind to TSHR.

Hyperthyroidism

Hyperthyroidism is when the thyroid is overactive which leads to upregulation of the metabolism. This overactive state can be initiated by antibodies like M22. When the M22 antibody is bound it keeps TSHR in the active conformation. When stuck in the active form, the thyroid continues to make T3 and T4, overstimulating the metabolism. Symptoms of hyperthyroidism include fast or irregular heartbeats, tiredness, increased hunger, sleep problems, enlarged thyroid gland, and sensitivity to heat. Grave's Disease is the most common cause of hyperthyroidism. This is an autoimmune disorder that causes your body to attack the thyroid gland^[6].

Hypothyroidism

Hypothyroidism is when the thyroid is underactive. When the K1 antibody is bound it does not allow the TSHR to go into the upright, active, position. This does not allow for the signaling of the T3 and T4 hormones to upregulate the metabolism. When stuck in the inactive form, TSH or M22 cannot bind to the TSHR to make more thyroid hormones which lead to an under-stimulation of the metabolism. The symptoms of this include slow or irregular heartbeats, tiredness, muscle aches, memory problems, jaundice, and sensitivity to cold. Hasimoto's disease is an example of hypothyroidism. This is an autoimmune disorder that causes your body to attack the healthy cells of the thyroid. Specifically causing the death of the cells that produce the thyroid hormones. When the thyroid fails to produce its hormones it activates TSH production through a negative feedback mechanism^[6]