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| | ==T316 complex== | | ==T316 complex== |
| - | <StructureSection load='4z7w' size='340' side='right' caption='[[4z7w]], [[Resolution|resolution]] 2.89Å' scene=''> | + | <StructureSection load='4z7w' size='340' side='right'caption='[[4z7w]], [[Resolution|resolution]] 2.89Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4z7w]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Canadian_hard_winter_wheat Canadian hard winter wheat] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z7W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z7W FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4z7w]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z7W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z7W FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4z7u|4z7u]], [[4z7v|4z7v]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z7w OCA], [https://pdbe.org/4z7w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z7w RCSB], [https://www.ebi.ac.uk/pdbsum/4z7w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z7w ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HLA-DQA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), HLA-DQB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z7w OCA], [http://pdbe.org/4z7w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z7w RCSB], [http://www.ebi.ac.uk/pdbsum/4z7w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4z7w ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q30069_HUMAN Q30069_HUMAN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 4z7w" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 4z7w" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Gluten|Gluten]] |
| | + | *[[T-cell receptor 3D structures|T-cell receptor 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Canadian hard winter wheat]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Human]] | + | [[Category: Large Structures]] |
| - | [[Category: Koning, F]] | + | [[Category: Triticum aestivum]] |
| - | [[Category: Petersen, J]] | + | [[Category: Koning F]] |
| - | [[Category: Reid, H H]] | + | [[Category: Petersen J]] |
| - | [[Category: Rossjohn, J]]
| + | [[Category: Reid HH]] |
| - | [[Category: Immune receptor-ligand complex]] | + | [[Category: Rossjohn J]] |
| - | [[Category: Immune system]] | + | |
| Structural highlights
Function
Q30069_HUMAN
Publication Abstract from PubMed
In HLA-DQ8-associated celiac disease (CD), the pathogenic T cell response is directed toward an immunodominant alpha-gliadin-derived peptide (DQ8-glia-alpha1). However, our knowledge of TCR gene usage within the primary intestinal tissue of HLA-DQ8+ CD patients is limited. We identified two populations of HLA-DQ8-glia-alpha1 tetramer+ CD4+ T cells that were essentially undetectable in biopsy samples from patients on a gluten-free diet but expanded rapidly and specifically after antigenic stimulation. Distinguished by expression of TRBV9, both T cell populations displayed biased clonotypic repertoires and reacted similarly against HLA-DQ8-glia-alpha1. In particular, TRBV9 paired most often with TRAV26-2, whereas the majority of TRBV9- TCRs used TRBV6-1 with no clear TRAV gene preference. Strikingly, both tetramer+/TRBV9+ and tetramer+/TRBV9- T cells possessed a non-germline-encoded arginine residue in their CDR3alpha and CDR3beta loops, respectively. Comparison of the crystal structures of three TRBV9+ TCRs and a TRBV9- TCR revealed that, as a result of distinct TCR docking modes, the HLA-DQ8-glia-alpha1 contacts mediated by the CDR3-encoded arginine were almost identical between TRBV9+ and TRBV9- TCRs. In all cases, this interaction centered on two hydrogen bonds with a specific serine residue in the bound peptide. Replacement of serine with alanine at this position abrogated TRBV9+ and TRBV9- clonal T cell proliferation in response to HLA-DQ8-glia-alpha1. Gluten-specific memory CD4+ T cells with structurally and functionally conserved TCRs therefore predominate in the disease-affected tissue of patients with HLA-DQ8-mediated CD.
Determinants of Gliadin-Specific T Cell Selection in Celiac Disease.,Petersen J, van Bergen J, Loh KL, Kooy-Winkelaar Y, Beringer DX, Thompson A, Bakker SF, Mulder CJ, Ladell K, McLaren JE, Price DA, Rossjohn J, Reid HH, Koning F J Immunol. 2015 May 6. pii: 1500161. PMID:25948817[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Petersen J, van Bergen J, Loh KL, Kooy-Winkelaar Y, Beringer DX, Thompson A, Bakker SF, Mulder CJ, Ladell K, McLaren JE, Price DA, Rossjohn J, Reid HH, Koning F. Determinants of Gliadin-Specific T Cell Selection in Celiac Disease. J Immunol. 2015 May 6. pii: 1500161. PMID:25948817 doi:http://dx.doi.org/10.4049/jimmunol.1500161
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