8wb2

From Proteopedia

(Difference between revisions)

Current revision

Heme-bound Arabidopsis thaliana temperature-induced lipocalin

Structural highlights

8wb2 is a 1 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.9Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TIL_ARATH Involved in basal (BT) and acquired thermotolerance (AT), probably by preventing plasma membrane lipids peroxidation induced by severe heat-shock (HS) (PubMed:19302169, PubMed:23837879). Lipocalin that confers protection against oxidative stress caused by heat, freezing, paraquat and light (PubMed:18671872, PubMed:19302169). Confers resistance to high salt (NaCl) levels, probably by protecting chloroplasts from ion toxicity via ion homeostasis maintenance (PubMed:20959419, PubMed:24028869). Required for seed longevity by ensuring polyunsaturated lipids integrity (PubMed:23837879).^[1] ^[2] ^[3] ^[4] ^[5]

Publication Abstract from PubMed

Arabidopsis thaliana temperature-induced lipocalin (AtTIL) is a prototypical member of plant lipocalins and participates in a variety of cellular processes, particularly stress responses. Bioinformatical and physiological studies have proposed its promiscuous ligand-binding ability, but the molecular basis is yet unclear. Here, we report the 1.9-A crystal structure of AtTIL in complex with heme. Spectrophotometric absorbance titration with heme yields a dissociation constant of approximately 2 micromolar, indicating the relatively weak interaction between AtTIL and heme, which is confirmed by the AtTIL-heme structure. Although binding to retinal or biliverdin is not detected, such possibility can not be precluded as suggested by comparison with other lipocalin structures. These results show that AtTIL is a structural and functional homolog of the bacterial lipocalin Blc.

Crystallographic and functional studies of a plant temperature-induced lipocalin.,Dong CS, Zhang WL, Wang XY, Wang X, Wang J, Wang M, Fang Y, Liu L Biochim Biophys Acta Gen Subj. 2023 Dec 14;1868(2):130540. doi: , 10.1016/j.bbagen.2023.130540. PMID:38103756^[6]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Charron JB, Ouellet F, Houde M, Sarhan F. The plant Apolipoprotein D ortholog protects Arabidopsis against oxidative stress. BMC Plant Biol. 2008 Jul 31;8:86. PMID:18671872 doi:10.1186/1471-2229-8-86
↑ Chi WT, Fung RW, Liu HC, Hsu CC, Charng YY. Temperature-induced lipocalin is required for basal and acquired thermotolerance in Arabidopsis. Plant Cell Environ. 2009 Jul;32(7):917-27. PMID:19302169 doi:10.1111/j.1365-3040.2009.01972.x
↑ Brinker M, Brosché M, Vinocur B, Abo-Ogiala A, Fayyaz P, Janz D, Ottow EA, Cullmann AD, Saborowski J, Kangasjärvi J, Altman A, Polle A. Linking the salt transcriptome with physiological responses of a salt-resistant Populus species as a strategy to identify genes important for stress acclimation. Plant Physiol. 2010 Dec;154(4):1697-709. PMID:20959419 doi:10.1104/pp.110.164152
↑ Boca S, Koestler F, Ksas B, Chevalier A, Leymarie J, Fekete A, Mueller MJ, Havaux M. Arabidopsis lipocalins AtCHL and AtTIL have distinct but overlapping functions essential for lipid protection and seed longevity. Plant Cell Environ. 2014 Feb;37(2):368-81. PMID:23837879 doi:10.1111/pce.12159
↑ Abo-Ogiala A, Carsjens C, Diekmann H, Fayyaz P, Herrfurth C, Feussner I, Polle A. Temperature-induced lipocalin (TIL) is translocated under salt stress and protects chloroplasts from ion toxicity. J Plant Physiol. 2014 Feb 15;171(3-4):250-9. PMID:24028869 doi:10.1016/j.jplph.2013.08.003
↑ Dong CS, Zhang WL, Wang XY, Wang X, Wang J, Wang M, Fang Y, Liu L. Crystallographic and functional studies of a plant temperature-induced lipocalin. Biochim Biophys Acta Gen Subj. 2023 Dec 14;1868(2):130540. PMID:38103756 doi:10.1016/j.bbagen.2023.130540

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/8wb2"

Categories: Arabidopsis thaliana | Large Structures | Dong C | Liu L

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 8wb2 is ON HOLD  until Paper Publication
+==Heme-bound Arabidopsis thaliana temperature-induced lipocalin==
+<StructureSection load='8wb2' size='340' side='right'caption='[[8wb2]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[8wb2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8WB2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8WB2 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8wb2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8wb2 OCA], [https://pdbe.org/8wb2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8wb2 RCSB], [https://www.ebi.ac.uk/pdbsum/8wb2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8wb2 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TIL_ARATH TIL_ARATH] Involved in basal (BT) and acquired thermotolerance (AT), probably by preventing plasma membrane lipids peroxidation induced by severe heat-shock (HS) (PubMed:19302169, PubMed:23837879). Lipocalin that confers protection against oxidative stress caused by heat, freezing, paraquat and light (PubMed:18671872, PubMed:19302169). Confers resistance to high salt (NaCl) levels, probably by protecting chloroplasts from ion toxicity via ion homeostasis maintenance (PubMed:20959419, PubMed:24028869). Required for seed longevity by ensuring polyunsaturated lipids integrity (PubMed:23837879).<ref>PMID:18671872</ref> <ref>PMID:19302169</ref> <ref>PMID:20959419</ref> <ref>PMID:23837879</ref> <ref>PMID:24028869</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Arabidopsis thaliana temperature-induced lipocalin (AtTIL) is a prototypical member of plant lipocalins and participates in a variety of cellular processes, particularly stress responses. Bioinformatical and physiological studies have proposed its promiscuous ligand-binding ability, but the molecular basis is yet unclear. Here, we report the 1.9-A crystal structure of AtTIL in complex with heme. Spectrophotometric absorbance titration with heme yields a dissociation constant of approximately 2 micromolar, indicating the relatively weak interaction between AtTIL and heme, which is confirmed by the AtTIL-heme structure. Although binding to retinal or biliverdin is not detected, such possibility can not be precluded as suggested by comparison with other lipocalin structures. These results show that AtTIL is a structural and functional homolog of the bacterial lipocalin Blc.
-Authors:
+Crystallographic and functional studies of a plant temperature-induced lipocalin.,Dong CS, Zhang WL, Wang XY, Wang X, Wang J, Wang M, Fang Y, Liu L Biochim Biophys Acta Gen Subj. 2023 Dec 14;1868(2):130540. doi: , 10.1016/j.bbagen.2023.130540. PMID:38103756<ref>PMID:38103756</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 8wb2" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Arabidopsis thaliana]]
+[[Category: Large Structures]]
+[[Category: Dong C]]
+[[Category: Liu L]]

8wb2

From Proteopedia

Current revision

Heme-bound Arabidopsis thaliana temperature-induced lipocalin

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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