6b4a

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of the C-Terminal Domain of Doublecortin (TgDCX) from Toxoplasma gondii ME49

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6b4a"

Categories: Large Structures | Toxoplasma gondii

@@ Line 3: / Line 3: @@
 <StructureSection load='6b4a' size='340' side='right'caption='[[6b4a]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6b4a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxgo Toxgo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B4A OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6B4A FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6b4a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B4A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6B4A FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BN1205_078130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5811 TOXGO])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6b4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b4a OCA], [http://pdbe.org/6b4a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b4a RCSB], [http://www.ebi.ac.uk/pdbsum/6b4a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b4a ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6b4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b4a OCA], [https://pdbe.org/6b4a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6b4a RCSB], [https://www.ebi.ac.uk/pdbsum/6b4a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6b4a ProSAT]</span></td></tr>
 </table>
-<div style="background-color:#fffaf0;">
+== Function ==
-== Publication Abstract from PubMed ==
+[https://www.uniprot.org/uniprot/DCXH_TOXGV DCXH_TOXGV] Specifically required in the formation and maintenance of the conoid fibers; the conoid is a component of the cytoskeletal apical complex, which is composed of a left-handed spiral of 14 fibers made from a nontubular tubulin polymer (PubMed:27932494). Promotes the organization, curvature, and stability of the conoid fibers, and probably bridges other conoid components to the tubulin core (PubMed:27932494, PubMed:32111164).<ref>PMID:27932494</ref> <ref>PMID:32111164</ref>
-BACKGROUND: TgDCX is a doublecortin-domain protein associated with the conoid fibers, a set of strongly curved non-tubular tubulin-polymers in Toxoplasma. TgDCX deletion impairs conoid structure and parasite invasion. TgDCX contains two tubulin-binding domains: a partial P25alpha and the DCX/doublecortin domain. Orthologues are found in apicomplexans and their free-living relatives Chromera and Vitrella. RESULTS: We report that isolated TgDCX-containing conoid fibers retain their pronounced curvature, but loss of TgDCX destabilizes the fibers. We crystallized and determined the 3D-structure of the DCX-domain, which is similar to those of human doublecortin and well-conserved among TgDCX orthologues. However, the orthologues vary widely in targeting to the conoid in Toxoplasma and in modulating microtubule organization in Xenopus cells. Several orthologues bind to microtubules in Xenopus cells, but only TgDCX generates short, strongly curved microtubule arcs. EM analysis shows microtubules decorated with TgDCX bundled into rafts, often bordered on one edge by a "C"-shaped incomplete tube. A Chromera orthologue closely mimics TgDCX targeting in Toxoplasma and binds to microtubules in Xenopus cells, but does not generate arcs or "C"-shaped tubes, and fails to rescue the defects of the TgDCX-knockout parasite. CONCLUSIONS: These observations suggest that species-specific features of TgDCX enable it to generate strongly curved tubulin-polymers to support efficient host-cell invasion.
-A doublecortin-domain protein of Toxoplasma and its orthologues bind to and modify the structure and organization of tubulin polymers.,Leung JM, Nagayasu E, Hwang YC, Liu J, Pierce PG, Phan IQ, Prentice RA, Murray JM, Hu K BMC Mol Cell Biol. 2020 Feb 28;21(1):8. doi: 10.1186/s12860-020-0249-5. PMID:32111164<ref>PMID:32111164</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 6b4a" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
@@ Line 22: / Line 15: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Toxgo]]
-[[Category: Structural genomic]]
-[[Category: Doublecortin]]
-[[Category: Ssgcid]]
-[[Category: Structural protein]]
-[[Category: Tgdcx]]
 [[Category: Toxoplasma gondii]]

6b4a

From Proteopedia

Current revision

Crystal structure of the C-Terminal Domain of Doublecortin (TgDCX) from Toxoplasma gondii ME49

Structural highlights

Function

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox