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| | ==NMR Structures of Yeast Proteasome Component Rpn13== | | ==NMR Structures of Yeast Proteasome Component Rpn13== |
| - | <StructureSection load='2z4d' size='340' side='right'caption='[[2z4d]], [[NMR_Ensembles_of_Models | 8 NMR models]]' scene=''> | + | <StructureSection load='2z4d' size='340' side='right'caption='[[2z4d]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2z4d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z4D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z4D FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2z4d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z4D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z4D FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ubq|1ubq]], [[2coc|2coc]], [[2dhk|2dhk]], [[2hth|2hth]], [[1fho|1fho]], [[2d9v|2d9v]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RPN13 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
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| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z4d OCA], [https://pdbe.org/2z4d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z4d RCSB], [https://www.ebi.ac.uk/pdbsum/2z4d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z4d ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z4d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z4d OCA], [https://pdbe.org/2z4d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z4d RCSB], [https://www.ebi.ac.uk/pdbsum/2z4d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z4d ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/RPN13_YEAST RPN13_YEAST]] Component of the 19S cap proteasome complex which acts as a regulatory subunit of the 26S proteasome, involved in the ATP-dependent degradation of ubiquitinated proteins.<ref>PMID:7957966</ref> <ref>PMID:11029046</ref>
| + | [https://www.uniprot.org/uniprot/RPN13_YEAST RPN13_YEAST] Component of the 19S cap proteasome complex which acts as a regulatory subunit of the 26S proteasome, involved in the ATP-dependent degradation of ubiquitinated proteins.<ref>PMID:7957966</ref> <ref>PMID:11029046</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 18824]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Walters, K J]] | + | [[Category: Saccharomyces cerevisiae]] |
| - | [[Category: Zhang, N]] | + | [[Category: Walters KJ]] |
| - | [[Category: Nuclear protein]] | + | [[Category: Zhang N]] |
| - | [[Category: Ph domain]]
| + | |
| - | [[Category: Proteasome]]
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| Structural highlights
Function
RPN13_YEAST Component of the 19S cap proteasome complex which acts as a regulatory subunit of the 26S proteasome, involved in the ATP-dependent degradation of ubiquitinated proteins.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Proteasomal receptors that recognize ubiquitin chains attached to substrates are key mediators of selective protein degradation in eukaryotes. Here we report the identification of a new ubiquitin receptor, Rpn13/ARM1, a known component of the proteasome. Rpn13 binds ubiquitin through a conserved amino-terminal region termed the pleckstrin-like receptor for ubiquitin (Pru) domain, which binds K48-linked diubiquitin with an affinity of approximately 90 nM. Like proteasomal ubiquitin receptor Rpn10/S5a, Rpn13 also binds ubiquitin-like (UBL) domains of UBL-ubiquitin-associated (UBA) proteins. In yeast, a synthetic phenotype results when specific mutations of the ubiquitin binding sites of Rpn10 and Rpn13 are combined, indicating functional linkage between these ubiquitin receptors. Because Rpn13 is also the proteasomal receptor for Uch37, a deubiquitinating enzyme, our findings suggest a coupling of chain recognition and disassembly at the proteasome.
Proteasome subunit Rpn13 is a novel ubiquitin receptor.,Husnjak K, Elsasser S, Zhang N, Chen X, Randles L, Shi Y, Hofmann K, Walters KJ, Finley D, Dikic I Nature. 2008 May 22;453(7194):481-8. PMID:18497817[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Fischer M, Hilt W, Richter-Ruoff B, Gonen H, Ciechanover A, Wolf DH. The 26S proteasome of the yeast Saccharomyces cerevisiae. FEBS Lett. 1994 Nov 21;355(1):69-75. PMID:7957966
- ↑ Verma R, Chen S, Feldman R, Schieltz D, Yates J, Dohmen J, Deshaies RJ. Proteasomal proteomics: identification of nucleotide-sensitive proteasome-interacting proteins by mass spectrometric analysis of affinity-purified proteasomes. Mol Biol Cell. 2000 Oct;11(10):3425-39. PMID:11029046
- ↑ Husnjak K, Elsasser S, Zhang N, Chen X, Randles L, Shi Y, Hofmann K, Walters KJ, Finley D, Dikic I. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature. 2008 May 22;453(7194):481-8. PMID:18497817 doi:10.1038/nature06926
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