This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1umg
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1umg.gif|left|200px]] | [[Image:1umg.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1umg", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | | | + | --> |
| - | | | + | {{STRUCTURE_1umg| PDB=1umg | SCENE= }} |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Crystal strucure of fructose-1,6-bisphosphatase''' | '''Crystal strucure of fructose-1,6-bisphosphatase''' | ||
| Line 30: | Line 27: | ||
[[Category: Shoun, H.]] | [[Category: Shoun, H.]] | ||
[[Category: Wakagi, T.]] | [[Category: Wakagi, T.]] | ||
| - | [[Category: | + | [[Category: Alpha-beta-beta-alpha four layer sandwich]] |
| - | [[Category: | + | [[Category: Fructose-1,6-bisphosphatase]] |
| - | [[Category: | + | [[Category: Hyperthermophilic archaea]] |
| - | [[Category: | + | [[Category: Magnesium ion]] |
| - | [[Category: | + | [[Category: Octamer]] |
| - | [[Category: | + | [[Category: Phosphatase]] |
| - | [[Category: | + | [[Category: Three metal-assisted mechanism]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:25:40 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 08:25, 3 May 2008
Crystal strucure of fructose-1,6-bisphosphatase
Overview
As the first structure of the novel class of fructose-1,6-bisphosphatase (FBPase) present in thermophilic archaea, we solved the crystal structure of the ST0318 gene product (St-Fbp) of Sulfolobus tokodaii strain 7. The St-Fbp structure comprises a homooctamer of the 422 point-group. The protein folds as a four-layer alpha-beta-beta-alpha sandwich with a novel topology, which is completely different from the sugar phosphatase fold. The structure contains an unhydrolyzed FBP molecule in the open-keto form, as well as four hexacoordinated magnesium ions around the 1-phosphoryl group of FBP. The arrangement of the catalytic side chains and metal ligands is consistent with the three-metal ion assisted catalysis proposed for conventional FBPases. The structure provides an insight into the structural basis of the strict substrate specificity of St-Fbp.
About this Structure
1UMG is a Single protein structure of sequence from Sulfolobus tokodaii. Full crystallographic information is available from OCA.
Reference
The first crystal structure of the novel class of fructose-1,6-bisphosphatase present in thermophilic archaea., Nishimasu H, Fushinobu S, Shoun H, Wakagi T, Structure. 2004 Jun;12(6):949-59. PMID:15274916 Page seeded by OCA on Sat May 3 11:25:40 2008
Categories: Fructose-bisphosphatase | Single protein | Sulfolobus tokodaii | Fushinobu, S. | Nishimasu, H. | Shoun, H. | Wakagi, T. | Alpha-beta-beta-alpha four layer sandwich | Fructose-1,6-bisphosphatase | Hyperthermophilic archaea | Magnesium ion | Octamer | Phosphatase | Three metal-assisted mechanism
