Synthetic nanomaterials from standardized protein blocks
From Proteopedia
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| - | In this project, the simplest building blocks consist of anti-parallel alpha helices engineered to be straight and flat, that is ''twistless helix repeat'' (THR) protein blocks. A simple example, THR1, is [[8g9j]], consisting of <scene name='10/1068508/8g9j_flat_square/3'>eight anti-parallel alpha helices with seven turns per helix</scene>. Each helix is amphipathic, that is, hydrophobic on the side contacting other helixes, and hydrophilic on the side facing outwards (not shown). The <scene name='10/1068508/8g9j_flat_square/2'>block surface is designed to have many charges</scene>, making a highly water soluble building block. The edges of the block are "capped" with | + | In this project, the simplest building blocks consist of anti-parallel alpha helices engineered to be straight and flat, that is ''twistless helix repeat'' (THR) protein blocks. A simple example, THR1, is [[8g9j]], consisting of <scene name='10/1068508/8g9j_flat_square/3'>eight anti-parallel alpha helices with seven turns per helix</scene><ref>An amino-terminal histidine tag was not resolved in the electron density map, and thus is missing in the structure depicted.</ref>. Each helix is amphipathic, that is, hydrophobic on the side contacting other helixes, and hydrophilic on the side facing outwards (not shown). The <scene name='10/1068508/8g9j_flat_square/2'>block surface is designed to have many charges</scene>, making a highly water soluble building block. The edges of the block are "capped" with charges that prevent these blocks from binding to each other, thus enabling crystallization of this block rather than having it precipitate. |
==Related Work== | ==Related Work== | ||
Revision as of 02:47, 4 January 2025
This page is under construction. This notice will be removed when it is ready. Eric Martz 21:20, 23 December 2024 (UTC) |
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References
- ↑ 1.0 1.1 1.2 Huddy TF, Hsia Y, Kibler RD, Xu J, Bethel N, Nagarajan D, Redler R, Leung PJY, Weidle C, Courbet A, Yang EC, Bera AK, Coudray N, Calise SJ, Davila-Hernandez FA, Han HL, Carr KD, Li Z, McHugh R, Reggiano G, Kang A, Sankaran B, Dickinson MS, Coventry B, Brunette TJ, Liu Y, Dauparas J, Borst AJ, Ekiert D, Kollman JM, Bhabha G, Baker D. Blueprinting extendable nanomaterials with standardized protein blocks. Nature. 2024 Mar;627(8005):898-904. PMID:38480887 doi:10.1038/s41586-024-07188-4
- ↑ An amino-terminal histidine tag was not resolved in the electron density map, and thus is missing in the structure depicted.
- ↑ Wang S, Favor A, Kibler R, Lubner J, Borst AJ, Coudray N, Redler RL, Chiang HT, Sheffler W, Hsia Y, Li Z, Ekiert DC, Bhabha G, Pozzo LD, Baker D. Bond-centric modular design of protein assemblies. bioRxiv [Preprint]. 2024 Oct 12:2024.10.11.617872. PMID:39416012 doi:10.1101/2024.10.11.617872
