9kjb
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Anti-CRISPR AcrIE7== | |
| + | <StructureSection load='9kjb' size='340' side='right'caption='[[9kjb]], [[Resolution|resolution]] 2.11Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9kjb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9KJB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9KJB FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.11Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9kjb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9kjb OCA], [https://pdbe.org/9kjb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9kjb RCSB], [https://www.ebi.ac.uk/pdbsum/9kjb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9kjb ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The CRISPR-Cas system is a well-known adaptive immune system in bacteria, and a prominent mechanism for evading this immunity involves anti-CRISPR (Acr) proteins, which employ various methods to neutralize the CRISPR-Cas system. In this study, using structural and biochemical analyses, we revealed that AcrIE7 binds to the single-stranded DNA in the R-loop formed when Cascade encounters the target DNA, thereby preventing Cas3 from cleaving the DNA. This represents a different inhibition strategy distinct from previously reported Acr mechanisms and offers insights into CRISPR-Cas inhibition. | ||
| - | + | AcrIE7 inhibits the CRISPR-Cas system by directly binding to the R-loop single-stranded DNA.,Kim DY, Lee SY, Ha HJ, Park HH Proc Natl Acad Sci U S A. 2025 Apr 8;122(14):e2423205122. doi: , 10.1073/pnas.2423205122. Epub 2025 Apr 3. PMID:40178896<ref>PMID:40178896</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 9kjb" style="background-color:#fffaf0;"></div> |
| - | [[Category: Kim | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas aeruginosa]] | ||
| + | [[Category: Kim DY]] | ||
| + | [[Category: Park HH]] | ||
Current revision
Crystal structure of Anti-CRISPR AcrIE7
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