1nmb
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(New page: 200px<br /> <applet load="1nmb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nmb, resolution 2.2Å" /> '''THE STRUCTURE OF A C...)
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Revision as of 07:31, 18 November 2007
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THE STRUCTURE OF A COMPLEX BETWEEN THE NC10 ANTIBODY AND INFLUENZA VIRUS NEURAMINIDASE AND COMPARISON WITH THE OVERLAPPING BINDING SITE OF THE NC41 ANTIBODY
Overview
BACKGROUND: While it is well known that different antibodies can be, produced against a particular antigen, and even against a particular site, on an antigen, up until now there have been no structural studies of, cross-reacting antibodies of this type. One antibody-antigen complex whose, structure is known is that of the influenza virus antigen, neuraminidase, in complex with the NC41 antibody. Another anti-neuraminidase antibody, NC10, binds to an overlapping site on the antigen. The structure of the, complex formed by this antibody with neuraminidase is described here and, compared with the NC41-containing complex. RESULTS: The crystal structure, of the NC10 Fab-neuraminidase complex has been refined to a nominal, resolution of 2.5A. Approximately 80% of the binding site of the NC10, antibody on neuraminidase overlaps with that of the NC41 antibody. The, epitope residues of neuraminidase are often engaged in quite different, interactions with the two antibodies. Although the NC10 and NC41, antibodies have identical amino acid sequences within the first, complementarity determining region of their heavy chains, this is not the, basis of the cross-reaction. CONCLUSIONS: The capacity of two different, proteins to bind to the same target structure on a third protein need not, be based on the existence of identical or homologous amino acid sequences, within those proteins. As we have demonstrated, amino acid residues on the, common target structure may be in quite different chemical environments, and may also adopt different conformations within two protein-protein, complexes.
About this Structure
1NMB is a Protein complex structure of sequences from Influenza virus a/whale/maine/1/84 isolated from pilot whale (globicephala melaena) and Mus musculus with NAG and CA as ligands. Active as Exo-alpha-sialidase, with EC number 3.2.1.18 Full crystallographic information is available from OCA.
Reference
The structure of a complex between the NC10 antibody and influenza virus neuraminidase and comparison with the overlapping binding site of the NC41 antibody., Malby RL, Tulip WR, Harley VR, McKimm-Breschkin JL, Laver WG, Webster RG, Colman PM, Structure. 1994 Aug 15;2(8):733-46. PMID:7994573
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