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1bb9

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{{STRUCTURE_1bb9| PDB=1bb9 | SCENE= }}
{{STRUCTURE_1bb9| PDB=1bb9 | SCENE= }}
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'''CRYSTAL STRUCTURE OF THE SH3 DOMAIN FROM RAT AMPHIPHYSIN 2'''
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===CRYSTAL STRUCTURE OF THE SH3 DOMAIN FROM RAT AMPHIPHYSIN 2===
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==Overview==
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The amphiphysins are brain-enriched proteins, implicated in clathrin-mediated endocytosis, that interact with dynamin through their SH3 domains. To elucidate the nature of this interaction, we have solved the crystal structure of the amphiphysin-2 (Amph2) SH3 domain to 2.2 A. The structure possesses several notable features, including an extensive patch of negative electrostatic potential covering a large portion of its dynamin binding site. This patch accounts for the specific requirement of amphiphysin for two arginines in the proline-rich binding motif to which it binds on dynamin. We demonstrate that the interaction of dynamin with amphiphysin SH3 domains, unlike that with SH3 domains of Grb2 or spectrin, prevents dynamin self-assembly into rings. Deletion of a unique insert in the n-Src loop of Amph2 SH3, a loop adjacent to the dynamin binding site, significantly reduces this effect. Conversely, replacing the n-Src loop of the N-terminal SH3 domain of Grb2 with that of Amph2 causes it to favour dynamin ring disassembly. Transferrin uptake assays show that shortening the n-Src loop of Amph2 SH3 reduces the ability of this domain to inhibit endocytosis in vivo. Our data suggest that amphiphysin SH3 domains are important regulators of the multimerization cycle of dynamin in endocytosis.
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(as it appears on PubMed at http://www.pubmed.gov), where 9736607 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9736607}}
==About this Structure==
==About this Structure==
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[[Category: Sh3 domain]]
[[Category: Sh3 domain]]
[[Category: Transferase]]
[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:18:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 18:45:19 2008''

Revision as of 15:45, 30 June 2008

Image:1bb9.png

Template:STRUCTURE 1bb9

CRYSTAL STRUCTURE OF THE SH3 DOMAIN FROM RAT AMPHIPHYSIN 2

Template:ABSTRACT PUBMED 9736607

About this Structure

1BB9 is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Crystal structure of the amphiphysin-2 SH3 domain and its role in the prevention of dynamin ring formation., Owen DJ, Wigge P, Vallis Y, Moore JD, Evans PR, McMahon HT, EMBO J. 1998 Sep 15;17(18):5273-85. PMID:9736607

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