User:Wayne Chang
From Proteopedia
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== Outline == | == Outline == | ||
| - | Glutamine synthetase catalyzes the condensation of glutamate and ammonia, | + | '''-- Work in Progress -- ''' |
| + | |||
| + | Glutamine synthetase (GS) catalyzes the ATP dependent condensation of glutamate and ammonia, producing, glutamine, ADP, and an inorganic phosphate group. | ||
Glutamate + ATP + NH3 → Glutamine + ADP + phosphate | Glutamate + ATP + NH3 → Glutamine + ADP + phosphate | ||
| + | |||
| + | Ammonium ion is thought to bind to GS at the monovalent cation binding site for Tl(+) and Cs(+) ions. | ||
== References == | == References == | ||
1. Liaw, S-H, et.al.,Discovery of the ammonium substrate site on glutamine synthetase, a third cation binding site Protein Sci. 1995 4: 2358-2365[http://www.proteinscience.org/cgi/content/abstract/4/11/2358] | 1. Liaw, S-H, et.al.,Discovery of the ammonium substrate site on glutamine synthetase, a third cation binding site Protein Sci. 1995 4: 2358-2365[http://www.proteinscience.org/cgi/content/abstract/4/11/2358] | ||
Revision as of 21:55, 15 November 2008
Contents |
Assignment 12: IVC: Ammonium Binding Site
Mapping the Ammonium binding site and explaining how it contributes to catalysis.
Chang, Wayne and Kaushal, Pankaj.
University of Maryland, Baltimore County (UMBC).
Script Exercises
|
Outline
-- Work in Progress --
Glutamine synthetase (GS) catalyzes the ATP dependent condensation of glutamate and ammonia, producing, glutamine, ADP, and an inorganic phosphate group.
Glutamate + ATP + NH3 → Glutamine + ADP + phosphate
Ammonium ion is thought to bind to GS at the monovalent cation binding site for Tl(+) and Cs(+) ions.
References
1. Liaw, S-H, et.al.,Discovery of the ammonium substrate site on glutamine synthetase, a third cation binding site Protein Sci. 1995 4: 2358-2365[1]
