User:Wayne Chang
From Proteopedia
(→Script Exercises) |
(→References) |
||
| Line 42: | Line 42: | ||
1. Liaw, S-H, et.al.,Discovery of the ammonium substrate site on glutamine synthetase, a third cation binding site Protein Sci. 1995 4: 2358-2365[http://www.proteinscience.org/cgi/content/abstract/4/11/2358] | 1. Liaw, S-H, et.al.,Discovery of the ammonium substrate site on glutamine synthetase, a third cation binding site Protein Sci. 1995 4: 2358-2365[http://www.proteinscience.org/cgi/content/abstract/4/11/2358] | ||
| + | |||
| + | 2. Liaw SH, Eisenberg D. Structural model for the reaction mechanism of glutamine synthetase, based on five crystal structures of enzyme-substrate complexes. Biochemistry. 1994 Jan 25;33(3):675-81. [http://www.ncbi.nlm.nih.gov/pubmed/7904828?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVLinkOut] | ||
Revision as of 22:03, 15 November 2008
Contents |
Assignment 12: IVC: Ammonium Binding Site
Mapping the Ammonium binding site and explaining how it contributes to catalysis.
Chang, Wayne and Kaushal, Pankaj.
University of Maryland, Baltimore County (UMBC).
Script Exercises
|
Exercise shows a backbone trace of Glutamate Synthase which allows the ligands inside ADP, P3S, Cl- and Mn2+ to be seen.
Isolates chain A of Glutamate Synthase and labels the ligands for easy identification.
Wire Structure of Active Site residues of chain A using information obtained from PDBsum entry for Glutamate Synthase.
Still picture of salt bridge between residue 63 of chain F and residue 319 of chain G. Bridge length is also provided in angstroms.
Outline
-- Work in Progress --
Glutamine synthetase (GS) catalyzes the ATP dependent condensation of glutamate and ammonia, producing, glutamine, ADP, and an inorganic phosphate group.
Glutamate + ATP + NH3 → Glutamine + ADP + phosphate
Ammonium ion is thought to bind to GS at the monovalent cation binding site for Tl(+) and Cs(+) ions.
References
1. Liaw, S-H, et.al.,Discovery of the ammonium substrate site on glutamine synthetase, a third cation binding site Protein Sci. 1995 4: 2358-2365[1]
2. Liaw SH, Eisenberg D. Structural model for the reaction mechanism of glutamine synthetase, based on five crystal structures of enzyme-substrate complexes. Biochemistry. 1994 Jan 25;33(3):675-81. [2]
