User:Wayne Chang

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 54: Line 54:
<scene name='User:Wayne_Chang/Ammonium_bs_big/1'>Ammonium Binding Site Whole Enzyme</scene>
<scene name='User:Wayne_Chang/Ammonium_bs_big/1'>Ammonium Binding Site Whole Enzyme</scene>
 +
<scene name='User:Wayne_Chang/Ammonium_binding_site/1'>Ammonium Binding Site</scene>
<scene name='User:Wayne_Chang/Ammonium_binding_site/1'>Ammonium Binding Site</scene>

Revision as of 06:27, 7 December 2008

Contents

Assignment 12: IVC: Ammonium Binding Site

Mapping the Ammonium binding site and explaining how it contributes to catalysis.

Chang, Yu-Wei and Kaushal, Pankaj.

BIOL 430: Biological Chemistry.

University of Maryland, Baltimore County (UMBC).



Script Exercises

PDB ID 2qc8

Drag the structure with the mouse to rotate

Exercise shows a backbone trace of Glutamate Synthase which allows the ligands inside ADP, P3S, Cl- and Mn2+ to be seen.


Isolates chain A of Glutamate Synthase and labels the ligands for easy identification.


Wire Structure of Active Site residues of chain A using information obtained from PDBsum entry for Glutamate Synthase.


Still picture of salt bridge between residue 63 of chain F and residue 319 of chain G. Bridge length is also provided in Angstroms.



Outline

PDB ID 2qc8

Drag the structure with the mouse to rotate


-- Work in Progress --

Glutamine synthetase (GS) catalyzes the ATP dependent condensation of glutamate and ammonia, producing, glutamine, ADP, and an inorganic phosphate group.

Glutamate + ATP + NH3 → Glutamine + ADP + phosphate

Ammonium ion is thought to bind to GS at the monovalent cation binding site for Tl(+) and Cs(+) ions.












References

1. Liaw, S-H, et.al.,Discovery of the ammonium substrate site on glutamine synthetase, a third cation binding site Protein Sci. 1995 4: 2358-2365[1]

2. Liaw SH, Eisenberg D. Structural model for the reaction mechanism of glutamine synthetase, based on five crystal structures of enzyme-substrate complexes. Biochemistry. 1994 Jan 25;33(3):675-81. [2]

Proteopedia Page Contributors and Editors (what is this?)

Wayne Chang, Eran Hodis

Personal tools