User:Tommie Hata/Introduction to Protein Engineering-Subtilisin

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<applet load='2sic' size='400' frame='true' align='right' caption='PDB ID 2sic, Subtilisin BPN' scene='User:Tommie_Hata/Introduction_to_Protein_Engineering-Subtilisin/Default-whole/1'/>
<applet load='2sic' size='400' frame='true' align='right' caption='PDB ID 2sic, Subtilisin BPN' scene='User:Tommie_Hata/Introduction_to_Protein_Engineering-Subtilisin/Default-whole/1'/>
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The enzyme to the right is subtilisin BPN' from PDB file 2sic. The subtilisin enzyme is colored white while the ''Streptomyces'' subtilisin inhibitor is colored yellow. By <scene name='User:Tommie_Hata/Introduction_to_Protein_Engineering-Subtilisin/Default/2'>minimizing the inhibitor to a fragment that is bound in the subtilisin active site</scene>, we can take a look at how the substrate binds the active site. Enzyme-inhibitor structures are more offen solved and deposited in the Protein Data Bank (PDB)compared to enzyme-substrate structures. This is because enzyme-substrate complexes are often transient by nature. Without being able to form a stable enzyme-substrate complex, crystals of the complex cannot be grown so that the structure can be solved through x-ray crystallography.
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The structure to the right is subtilisin BPN' and streptomyces subtilisin inhibitor (PDB ID: 2sic). The subtilisin enzyme is colored white while the ''Streptomyces'' subtilisin inhibitor is colored yellow. By <scene name='User:Tommie_Hata/Introduction_to_Protein_Engineering-Subtilisin/Default/3'>minimizing the inhibitor to a fragment that is bound in the subtilisin active site</scene>, we can take a look at how the substrate binds the active site. Enzyme-inhibitor structures are more offen solved and deposited in the Protein Data Bank (PDB)compared to enzyme-substrate structures. This is because enzyme-substrate complexes are often transient by nature. Without being able to form a stable enzyme-substrate complex, crystals of the complex cannot be grown so that the structure can be solved through x-ray crystallography.

Revision as of 14:42, 6 August 2009

This Protein Engineering module has been developed using material from Dr. Scott Banta's course, Protein Engineering (chemical engineering department at Columbia University).

PDB ID 2sic, Subtilisin BPN

Drag the structure with the mouse to rotate

The structure to the right is subtilisin BPN' and streptomyces subtilisin inhibitor (PDB ID: 2sic). The subtilisin enzyme is colored white while the Streptomyces subtilisin inhibitor is colored yellow. By , we can take a look at how the substrate binds the active site. Enzyme-inhibitor structures are more offen solved and deposited in the Protein Data Bank (PDB)compared to enzyme-substrate structures. This is because enzyme-substrate complexes are often transient by nature. Without being able to form a stable enzyme-substrate complex, crystals of the complex cannot be grown so that the structure can be solved through x-ray crystallography.


, which has been minimized in the view to highlight the chain bound in the active site of subtilisin.


Reference

  • Takeuchi Y, Satow Y, Nakamura KT, Mitsui Y. Refined crystal structure of the complex of subtilisin BPN' and Streptomyces subtilisin inhibitor at 1.8 A resolution. J Mol Biol. 1991 Sep 5;221(1):309-25. PMID:1920411

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Tommie Hata

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