Ribosomal protein S12

From Proteopedia

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Ribosomal protein S12 (RPS12) is one of the 22 proteins which belong to the small subunit of the bacterial ribosome. It is involved in the translation initiation step. The images at the left and at the right correspond to one representative RPS12, i.e. the crystal structure of Ribosomal protein S12 from Thermus thermophilus (2zm6, chain L).

3D Structures of Ribosomal protein S12

Updated on 11-March-2013

RPS12

1qzc - TtRPS12+16S RRNA helix+23S RRNA helix - Thermus thermophilus
1pn7 – TtRPS12+TtRPL11+P-site tRNA+16S RRNA fragment+23S RRNA fragment
1pn8 - TtRPS12+TtRPL11+E-site tRNA+16S RRNA fragment+23S RRNA fragment
1mvr – EcRPS12+EcRPL11+mRNA codon+16S RRNA fragment+23S RRNA fragment – Cryo-EM - Escherichia coli

S12 in small ribosomal subunit

2zm6, 1n36, 1j5e, 3oto - TtRPS12 in 30S ribosomal subunit+THX+16S RRNA
2vqe, 2vqf, 2uuc, 2e5l, 2uu9, 2uua, 2uub, 3pyn, 3pyq, 3pys, 3pyu - TtRPS12 in 30S ribosomal subunit+16S RRNA+RNA fragment
3kc4 – EcRPS12 in 30S ribosomal subunit
3i1m, 3i1o, 3df1, 3df3, 2vho, 2vhp, 2avy, 2aw7, 1pnx, 3or9, 3ora - EcRPS12 in 30S ribosomal subunit+16S RRNA
1i94, 1fka, 3uxs, 3uxt, 4dr1 - TtRPS12 in 30S ribosomal subunit+16S RRNA
4dr4 – TtRPS12 in 30S ribosomal subunit+16S RRNA + mRNA
1p87, 1p6g - EcRPS12 in 30S ribosomal subunit+16S RRNA – Cryo-EM
3bbn - RPS12 in 30S ribosomal subunit+16S RRNA – Cryo-EM – spinach

S12 in small + large ribosomal subunit

1ml5 - EcRPS12 in 30S ribosomal subunit+50S ribosomal subunit+THX+chain release factor 2+P-site tRNA+tRNA Phe+mRNA fragment+23S RRNA+5S RRNA+16S RRNA - EM

S12 in small ribosomal subunit+ chain release factor

3mr8, 3ms0 – TtRPS12 in 30S ribosomal subunit+THX+chain release factor 1+P-site tRNA+mRNA+16S RRNA
3d5a, 3d5c - TtRPS12 in 30S ribosomal subunit+THX+chain release factor 1+P-site tRNA+E-site tRNA+mRNA +16S RRNA
3zvo – TtRPS12 in 30S ribosomal subunit+THX+P-site tRNA Met +mRNA +16S RRNA+chain release factor 3
2b64 - TtRPS12 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA Phe+mRNA +16S RRNA+chain release factor 1
2b9m - TtRPS12 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA Phe+mRNA +16S RRNA+chain release factor 2
2x9r, 2x9t, 2wh1, 2wh3 - TtRPS12 in 30S ribosomal subunit+THX+chain release factor 2+P-site tRNA+mRNA +16S RRNA
3f1e, 3f1g - TtRPS12 in 30S ribosomal subunit+THX+chain release factor 2+P-site tRNA+E-site tRNA +16S RRNA
3uoq - EcRPS12 + 16S RRNA + mRNA + peptide chain release factor

S12 in small ribosomal subunit+tRNA+mRNA

2hgi - TtRPS12 in 30S ribosomal subunit+16S RRNA+tRNA Phe+tRNA fMet
2hgp - TtRPS12 in 30S ribosomal subunit+16S RRNA+tRNA Phe+mRNA
3t1h, 3t1y - TtRPS12 in 30S ribosomal subunit+16S RRNA+tRNA Lys +mRNA
3tvf, 3tvg, 3uyd, 3uyf - TtRPS12 in 30S ribosomal subunit+16S RRNA+tRNA Leu + tRNA fMet + mRNA
3uz6, 3uz7, 3uzg, 3uzi - TtRPS12 in 30S ribosomal subunit+16S RRNA+tRNA Tyr + tRNA fMet + mRNA
2i2p, 2i2u - EcRPS12 in 30S ribosomal subunit+16S RRNA+tRNA Phe+mRNA
2hgr - TtRPS12 in 30S ribosomal subunit+16S RRNA+tRNA Phe+tRNA fMet+mRNA
1yl4 – TtRPS12 in 30S ribosomal subunit+ tRNA Phe+16S RRNA+THRS mRNA operator
1pns - EcRPS12 in 30S ribosomal subunit+ tRNA Phe+16S RRNA+mRNA
3j0u, 3j0v, 3j0x, 3j0z, 3j10, 3j20 – EcRPS12 in 30S ribosomal subunit+ A-site tRNA + P-site tRNA +16S RRNA+mRNA – Cryo-EM
3j13 – EcRPS12 in 30S ribosomal subunit+ P-site tRNA +16S RRNA+mRNA – Cryo-EM
1xnq, 1xnr - TtRPS12 in 30S ribosomal subunit+THX+mRNA+tRNA anticodon+16S RRNA

S12 in small ribosomal subunit+A-site tRNA

2uxd, 2uxc - TtRPS12 in 30S ribosomal subunit+THX+A-site mRNA CGGG+tRNA anticodon+16S RRNA
1n32, 1n33 - TtRPS12 in 30S ribosomal subunit+THX+A-site mRNA fragment+tRNA anticodon+16S RRNA
1n34 - TtRPS12 in 30S ribosomal subunit+THX+A-site mRNA fragment +16S RRNA
1xmo, 1xmq - TtRPS12 in 30S ribosomal subunit+THX+A-site mRNA+tRNA anticodon+16S RRNA
2uxb - TtRPS12 in 30S ribosomal subunit+THX+A-site mRNA GGGU+tRNA anticodon+16S RRNA

S12 in small ribosomal subunit+P-site tRNA

3i8g - TtRPS12 in 30S ribosomal subunit+P-site tRNA+mRNA +16S RRNA
3i8h, 3i9b, 3i9d - TtRPS12 in 30S ribosomal subunit+THX+P-site tRNA+mRNA +16S RRNA
1ibk - TtRPS12 in 30S ribosomal subunit+THX+P-site mRNA fragment +16S RRNA
2wwl - EcRPS12 in 30S ribosomal subunit+Poly-Ala chain+P-site tRNA+mRNA +16S RRNA - Escherichia coli
3i1q - EcRPS12 in 30S ribosomal subunit+P-site tRNA hairpin+mRNA
3i1s, 3i1z, 3i21 - EcRPS12 in 30S ribosomal subunit+P-site tRNA hairpin+mRNA +16S RNA

S12 in small ribosomal subunit+A-site tRNA+P-site tRNA

1ibl, 1ibm - TtRPS12 in 30S ribosomal subunit+THX+A-site mRNA fragment+P-site mRNA fragment+tRNA anticodon+16S RRNA

S12 in small ribosomal subunit+P-site tRNA+E-site tRNA

2ow8 - TtRPS12 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA+mRNA +16S RRNA
2b9o - TtRPS12 in 30S ribosomal subunit+THX+P-site tRNA Phe+E-site tRNA Phe+mRNA +16S RRNA
2qnh - TtRPS12 in 30S ribosomal subunit+THX+P-site tRNA+E-site tRNA+mRNA +16S RRNA

S12 in small ribosomal subunit+A-site tRNA+P-site tRNA+E-site tRNA

2gy9, 2gyb - EcRPS12 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA – Cryo-EM
1jgo, 1jgp, 1jgq, 1gix - TtRPS12 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+tRNA Phe+mRNA +16S RRNA
3e1a, 3e1c - EcRPS12 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA

S12 in small ribosomal subunit + antibiotics

1i95, 1i97, 2hhh, 2f4v , 3oge, 3ogy, 3ohc, 3ohd, 3ohy, 3oi0, 3oi2, 3oi4, 4dr2, 4dr3 - TtRPS12 in 30S ribosomal subunit+16S RRNA+antibiotic
1hnw, 1hnx, 1hnz, 1fjg, 4aqy, 4dr5, 4dr6, 4dr7 - TtRPS12 in 30S ribosomal subunit+16S RRNA+mRNA fragment+antibiotic
2qbb, 2qb9, 1vs5, 1vs7, 2qan, 2qal, 2qou, 2qow, 2qoy, 2qp0, 2z4k, 2z4m, 3oaq, 3oar, 3ofa, 3ofb, 3ofo, 3ofp, 3ofx, 3ofy - EcRPS12 in 30S ribosomal subunit+16S RRNA+antibiotic
3sfs – EcRPS12 in 30S ribosomal subunit+16S RRNA+ mRNA + RF-3 + antibiotic
4gaq, 4gas – EcRPS12 in 30S ribosomal subunit+16S RRNA+ mRNA + antibiotic
2j00, 2j02 - TtRPS12 in 30S ribosomal subunit+16S RRNA+paromomycin+P-site tRNA fMet+E-site tRNA Phe+mRNA
2wdg, 2wdh, 2wdk, 2wdm - TtRPS12 in 30S ribosomal subunit+THX+P-site tRNA+E-site tRNA+mRNA +16S RRNA+tRNA Phe+paromomycin
3knj, 3knh - TtRPS12 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA+viomycin
3knl, 3knn - TtRPS12 in 30S ribosomal subunit+P-site tRNA+A-site tRNA+E-site tRNA+mRNA +16S RRNA+capreomycin
3uz3, 3uz4 - TtRPS12 in 30S ribosomal subunit+16S RRNA+tRNA Leu + tRNA fMet + mRNA + paromomycin
3uzl, 3uzm - TtRPS12 in 30S ribosomal subunit+16S RRNA+tRNA Tyr + tRNA fMet + mRNA + paromomycin

S12 in small ribosomal subunit + ribosome recycling factor

2qbd, 2qbf – EcRPS12 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor
3r8n, 3r8o, 4gd1, 4gd2 – EcRPS12 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor + mRNA + tRNA
2qbh, 2qbj - EcRPS12 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor+gentamicin
3j0d, 3j0e - EcRPS12 + RPL11 +16S RRNA + 23S RRNA +ribosome recycling factor – Cryo EM
2v46, 2v48 - TtRPS12 in 30S ribosomal subunit+16S RRNA+ribosome recycling factor+P-site tRNA Phe+mRNA+tRNA fMet
1zn1 - TtRPS12+16S RRNA fragment+23S RRNA fragment+ribosome recycling factor+ chain release factor

S12 in small ribosomal subunit + elongation factor

2wri, 2wrk - TtRPS12 in 30S ribosomal subunit+THX+P-site tRNA+mRNA +16S RRNA+elongation factor G
2wrn, 2wrq - TtRPS12 in 30S ribosomal subunit+THX+A-site tRNA+mRNA +16S RRNA+elongation factor TU-4
3fic - TtRPS12 in 30S ribosomal subunit+THX+A-site tRNA+P-site tRNA+E-site tRNA+mRNA codon+16S RRNA+elongation factor TU-A
4abr - TtRPS12 in 30S ribosomal subunit+THX + E/P-site tRNA fMet +mRNA codon+16S RRNA+elongation factor TU
3izv – EcRPS12 in 30S ribosomal subunit+A/T-site tRNA Phe +16S RRNA+elongation factor TU
3j18 - EcRPS12 in 30S ribosomal subunit+A/T-site tRNA Phe +16S RRNA+ mRNA + SSRA-binding protein + formyl-Met specific initiator tRNA + elongation factor G – Cryo-EM
3izw – EcRPS12 in 30S ribosomal subunit+A-site tRNA Phe +mRNA +16S RRNA+elongation factor TU
2y0u, 2y0w, 2y0y, 2y10, 2y12, 2y14, 2y16, 2y18 - TtRPS12 in 30S subunit+THX+A-site tRNA+E-site tRNA+mRNA codon+ 16S RRNA+elongation factor TU
3fih - EcRPS12 in 30S ribosomal subunit+A-site tRNA Phe+P-site tRNA+mRNA +16S RRNA+elongation factor TU
3huw, 3huy - TtRPS12 in 30S ribosomal subunit+THX+tRNA-Met+mRNA +16S RRNA+elongation factor P
3ep2, 3eq3, 3eq4 – EcRPS12+EcRPL11+16S RRNA fragment+23S RRNA fragment+tRNA+elongation factor TU
2om7 - TtRPS12+TtRPL1+16S RRNA fragment+23S RRNA fragment+tRNA+elongation factor G
1mj1 – EcRPS12+EcRPS13+EcRPL11+16S RRNA fragment+23S RRNA fragment+tRNA Phe+elongation factor TU – Cryo-EM

S12 in small ribosomal subunit + translation initiation factor

1i96 - TtRPS12 in 30S ribosomal subunit+THX+IF3+16S RRNA
1hr0 - TtRPS12 in 30S ribosomal subunit+THX+IF+16S RRNA

S12 in small ribosomal subunit + various proteins

3kiq, 3kis, 3kiu, 3kix - TtRPS12 in 30S ribosomal subunit+toxin RelE+16S RRNA
4dh9, 4dhb - TtRPS12 in 30S ribosomal subunit +16S RRNA+mRNA+P-site tRNA fMet +THX + YAEJ
1voq, 1vos, 1vov, 1vox, 1voz - EcRPS12 in 30S ribosomal subunit+protein Y+16S RRNA
2xfz, 2xg1 - TtRPS12 in 30S ribosomal subunit+tRNA Phe+mRNA+colicin E3+16S RRNA
3deg - EcRPS12+EcRPL11+16S RRNA fragment+23S RRNA fragment+A-site tRNA+P-site tRNA+LEPA EF4 fragment
3j00 – EcRPS12 + 16S RRNA + FTSQ + apolipoprotein A-I
2xzm, 2xzn - RPS12 in 40S subunit+18S RRNA+RACK1 Tetrahymena thermophila
2ykr – EcRPS12 + 16S RRNA + GMPPNP + GTPase RSGA
4a2i – EcRPS12 + 16S RRNA + GTPase RSGA – Cryo EM
4adv - EcRPS12 + 16S RRNA + methyltransferase – Cryo EM
3v22, 3v24, 3v26, 3v28 – TtRPS12 in 30S ribosomal subunit +16S RRNA +THX + modulation factor
3v2c, 3v2e – TtRPS12 in 30S ribosomal subunit +16S RRNA +THX + ribosome-associated inhibitor
3iz6 - RPS12 in 40S ribosomal subunit+RACK1 – wheat – Cryo EM
3izb - yRPS12 in 40S ribosomal subunit+ RACK1 – yeast - Cryo-EM
3u5c, 3u5g - yRPS8 in 40S ribosomal subunit+ RACK1 + suppressor protein SMT1

S12 in part of small ribosomal subunit

3iy8 - EcRPS12+EcRPS5+EcRPS6+EcRPS9+EcRPS9 EcRPS8+EcRPS11+EcRPS15+EcRPS16+EcRPS17+EcRPS18+Leishmania taretolae mRNA
2r1g – TtRPS12+ TtRPS9+ TtRPS13+ 16S RRNA fragment

Related Structures and Topics

• LepA
• The Ribosome
• For additional information, see: Translation