This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

Chaperonin

From Proteopedia

Revision as of 07:23, 26 August 2014 by Michal Harel (Talk | contribs)

(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)

Jump to: navigation, search

Crystal Structure of Chaperonin, 1pcq

Template:STRUCTURE 1pcq

Chaperonins (CPN) are oligomeric proteins that mediate the folding of polypeptide chains. Group I CPN are found in bacteria, chloroplasts and mitochondria. For an introductory overview, see Chaperonins in Wikipedia.

The most characterized CPN are in the GroEL/GroES complex from Escherichia coli and CPN60/CPN10 from Thermus thermophilus. The larger subunit (GroEL, CPN60) contains 3 domains. The apical domain is the one which binds the substrate. Group II CPNs are found in eukaryotic cytosol and archaea. Thermosome is a CPN complex found in archaea. CCT is a CPN complex found in eukarya.

1 3D Structures of Chaperonin

3D Structures of Chaperonin

Updated on 26-August-2014

Group I

Large subunit

3m6c – MtGroEL1 apical domain – Mycobacterium tuberculosis
1sjp, 3rtk – MtGroEL2 residues 42-539
3fbh, 2eu1, 1j4z, 1kpo, 1oel, 1grl, 2yey – EcGroEL (mutant) - Escherichia coli
3e76, 2nwc, 1xck, 1ss8, 4hel – EcGroEL
3c9v, 3cau, 2ynj – EcGroEL – Cryo EM
2c7e, 1gr5, 4aaq - EcGroEL (mutant) – Cryo EM
1dk7 - EcGroEL apical domain
1fy9, 1fya, 1kid, 1jon, 3vz6, 3vz7, 3vz8 – EcGroEL apical domain (mutant)
1srv – TtCPN60 apical domain - Thermus thermophilus
3osx - CPN60 apical domain – Xenorhabdus nematophila
1iok – CPN60 – Paracoccus denitrificans

Large subunit binary complex

1kp8 - EcGroEL (mutant) + ATP
4aar, 4aas, 4aau, 4ab2, 4ab3 - EcGroEL (mutant) + ATP – Cryo EM
1sx3 - EcGroEL + ATP
1sx4 - EcGroEL + ADP
4ki8 - EcGroEL (mutant) + ADP
1mnf – EcGroEL + polypeptide
2cgt – EcGroEL + capsid assbly protein GP31 – EM
1dkd - EcGroEL apical domain + polypeptide

Small subunit

3nx6 – GroES residues 40-134 (mutant) – Xanthomonas oryzae
1wnr – TtCPN10 residues 1-94
1p3h, 1hx5 – MtCPN10
1p82, 1p83 - MtCPN10 residues 1-25 - NMR
1egs - GroES residues 19-27 - NMR

Large + small subunit

1svt, 1pcq - EcGroEL + GroES
1pf9, 1aon - EcGroEL + GroES + ADP
2c7c, 2c7d - EcGroEL + GroES – Cryo EM
1gru - EcGroEL + GroES + ATP + ADP – Cryo EM
3zpz, 3zq0, 3zq1 - EcGroEL + GroES + ADP – Cryo EM
1we3 – TtCPN60 + CPN10
1wf4 - TtCPN60 + CPN10 + ADP

Group II

3izh, 3izi, 3izj, 3izk, 3izl, 3izm, 3izn, 3los, 3iyf, 3j3x – MmCPN – Methanococcus maripaludis – EM
3kfb, 3kfe, 3kfk – MmCPN
3j02, 3j03 – MmCPN (mutant) – Cryo EM
3ruq - MmCPN + ADP
3rus - MmCPN (mutant) + ADP
3ruw - MmCPN (mutant) + ADP-AlF3
3ruv - MmCPN (mutant) + ATP analog

CCT

2xsm, 4b2t – bCPN CCT – bovine
3p9d, 3p9e, 4d8q, 4d8r, 4aol, 4apk – CPN CCT - yeast
3iyg, 4a0o - bCPN CCT – Cryo EM
4a0v - bCPN CCT + AMPPNP – Cryo EM
4a0w - bCPN CCT + ADP + AlF3 – Cryo EM
4a13 - bCPN CCT + ADP – Cryo EM
3ktt - bCPN CCT β subunit – Cryo EM
1gml, 1gn1 - CPN CCT γ subunit apical domain – mouse

Hsp33

See Heat Shock Proteins

Thermosome

1a6d - TaTherm α+β subunits – Thermoplasma acidophilum
1a6e - TaTherm α+β subunits + ADP
1ass, 1asx - TaTherm α apical domain
1e0r – TaTherm β apical domain
3ko1 – AtTherm α subunit– Acidianus tengchongensis
3j1b, 3j1c, 3j1e - AtTherm α subunit – Cryo EM
3j1f - AtTherm β subunit + ATP – Cryo EM
3aq1 – Therm – Methanococcoides burtonii
1q2v, 1q3r – TkTherm α subunit (mutant) – Thermococcus KS-1
1q3q - TkTherm α subunit (mutant) + AMP-PNP
1q3s - TkTherm α subunit (mutant) + ADP