Resolvase
From Proteopedia
Function
Resolvase or recombinase (Rec) is a nuclease which is involved in DNA recombination. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase.
- Holliday junction resolvase (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands[1].
- Tyr-recombinases include Cre which recombines loxP sites, FLP and lambda integrase (LamInt).
- Ser-recombinases (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase.
- RadA recombinase promotes DNA recombination. Detailed analysis of its suggests mechanisms for junction isomerization and communication between the two active sites [2].
- Hin-recombinase (HRec) is a protein of Salmonella. HRec inverts a 900 base pair DNA segment which contains the promoters of flagellar genes. The inversion changes the these genes' expressions[3].
3D structures of resolvase
Updated on 04-August-2016
References
- ↑ West SC. The search for a human Holliday junction resolvase. Biochem Soc Trans. 2009 Jun;37(Pt 3):519-26. doi: 10.1042/BST0370519. PMID:19442245 doi:http://dx.doi.org/10.1042/BST0370519
- ↑ Ceschini S, Keeley A, McAlister MS, Oram M, Phelan J, Pearl LH, Tsaneva IR, Barrett TE. Crystal structure of the fission yeast mitochondrial Holliday junction resolvase Ydc2. EMBO J. 2001 Dec 3;20(23):6601-11. PMID:11726496 doi:10.1093/emboj/20.23.6601
- ↑ Dhar G, Sanders ER, Johnson RC. Architecture of the hin synaptic complex during recombination: the recombinase subunits translocate with the DNA strands. Cell. 2004 Oct 1;119(1):33-45. PMID:15454079 doi:http://dx.doi.org/10.1016/j.cell.2004.09.010
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