Chaperonin

From Proteopedia

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Template:STRUCTURE 1pcq

Chaperonin (CPN) are oligomeric proteins which mediate the folding of polypeptide chains. Group I CPNs are found in bacteria, chloroplasts and mitochondria. They include the most characterized GroEL/GroES complex from Escherichia coli and CPN60/CPN10 from Thermus thermophilus. The larger subunit (GroEL, CPN60) contains 3 domains. The apical domain is the one which binds the substrate. Group II CPNs are found in eukaryotic cytosol and archaea. Thermosome is a CPN complex found in archaea. CCT is a CPN complex found in eukarya.

3D Structures of Chaperonin

Update June 2011

Group I

Large subunit

3m6c – MtGroEL1 apical domain – Mycobacterium tuberculosis
1sjp – MtGroEL2 residues 42-539
3fbh, 2eu1, 1j4z, 1kpo, 1oel, 1grl, 2yey – EcGroEL (mutant) - Escherichia coli
3e76, 2nwc, 1xck, 1ss8 – EcGroEL
3c9v, 3cau – EcGroEL – Cryo EM
2c7e, 1gr5 - EcGroEL (mutant) – Cryo EM
1kp8 - EcGroEL (mutant) + ATP
1sx3 - EcGroEL + ATP
1sx4 - EcGroEL + ADP
1mnf – EcGroEL + polypeptide
2cgt – EcGroEL + capsid assbly protein GP31 – EM
1fy9, 1fya, 1kid – EcGroEL apical domain (mutant)
1srv – TtCPN60 apical domain - Thermus thermophilus
1iok – CPN60 – Paracoccus denitrificans

Small subunit

3nx6 – GroES residues 40-134 (mutant) – Xanthomonas oryzae
1wnr – TtCPN10 residues 1-94
1p3h, 1hx5 – MtCPN10
1p82, 1p83 - MtCPN10 residues 1-25 - NMR
1egs - GroES residues 19-27 - NMR

Large + small subunit

1svt, 1pcq - EcGroEL + GroES
1pf9, 1aon - EcGroEL + GroES + ADP
2c7c, 2c7d - EcGroEL + GroES – Cryo EM
1gru - EcGroEL + GroES + ATP + ADP – Cryo EM
1we3 – TtCPN60 + CPN10
1wf4 - TtCPN60 + CPN10 + ADP

Group II

3izh, 3izi, 3izj, 3izk, 3izl, 3izm, 3izn, 3los, 3iyf – MmCPN – Methanococcus maripaludis – EM
3kfb, 3kfe, 3kfk – MmCPN
1q2v, 1q3r – TkCPN α subunit (mutant) – Thermococcus KS-1
1q3q - TkCPN α subunit (mutant) + AMP-PNP
1q3s - TkCPN α subunit (mutant) + ADP

CCT

2xsm – bCPN CCT – bovine
3iyg - bCPN CCT – Cryo EM
3ktt - bCPN CCT β subunit – Cryo EM
1gml, 1gn1 - CPN CCT γ subunit apical domain – mouse

Hsp33

3m7m, 1hw7 – EcCPN Hsp33 N-terminal
1xjh - EcCPN Hsp33 C terminal - NMR
1vq0 - CPN Hsp33 – Thermotoga maritime
1vzy - CPN Hsp33 (mutant) – Bacillus subtilis

Thermosome

1a6d - TaTherm α+β subunits – Thermoplasma acidophilum
1a6e - TaTherm α+β subunits + ADP
1ass, 1asx - TaTherm α apical domain
1e0r – TaTherm β apical domain
3ko1 – Therm – Acidianus tengchongensis 1lep – CPN-10 – Mycobacterium leprae