Sandbox 206
From Proteopedia
Contents |
A new YFP called Venus (1MYW)
Overview
In this article we want to present you a new YFP (Yellow Fluorescent Protein) with improved maturation and reduced environmental sensitivity due to 5 mutation of the well characterized variant of the YFP that is to say the EYFP (Enhanced Yellow Fluorescent Protein). The PDB number of this molecule is 1MYW.
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From the GFP to the EYFP
Presentation of the molecule
Primary and Secondary structure
The different mutations
Other Mutant of YFP
You can also see on the GFP main page (here), many others Fluorescence Proteins.
Applications
Reference for this Structure
Rekas A, Alattia JR, Nagai T, Miyawaki A, Ikura M. Crystal structure of venus, a yellow fluorescent protein with improved maturation and reduced environmental sensitivity. J Biol Chem. 2002 Dec 27;277(52):50573-8. Epub 2002 Oct 4.
Pubmed : 12370172
DOI : 10.1074/jbc.M209524200
Experimental Details of the Crystallization
Method: X-RAY DIFFRACTION
Exp. Data: N/A
Resolution: 2.20 Å
R-Value: 0.218 (obs.)
R-Free: 0.248
Unit Cell :
| Length [Å] | Angles [Å] |
|---|---|
| a = 82.70 | α = 90.00 |
| b = 82.70 | β = 90.00 |
| c = 72.56 | γ = 120.00 |
This information is on the PDB page : here
References
Additional Resources
Nikon : http://www.microscopyu.com/print/articles/livecellimaging/fpintro-print.html
