Exoenzyme

Function

Exoenzymes (Exo) are secreted by cells and break large macromolecules outside the cells. The breakdown components can pass through the cell wall. Exo C3 is a Clostridium butulinum toxin which binds NAD, splits it into ADP-ribose and nicotinamide and transfers the ADP-ribose to a protein substrate. Exo S and Exo T are secreted by Pseudomonas aeruginosa and has GTPase-activating activity as well as ADP-ribosyltransferase activity.

Disease

Relevance

Structural highlights

3D structures of exoenzyme

Updated on 18-August-2014

Exoenzyme C3

1g24 – CbExo residues 8-218 – Clostridium butulinum
1uzi, 1r45, 1r4b, 2c89 – CbExo catalytic domain
1gze, 2c8b, 2c8d, 2c8e, 2c8g – CbExo catalytic domain (mutant)
3bw8 - Exo catalytic domain - Clostridium limosum

Exoenzyme C3 complexes

1gzf – CbExo catalytic domain + pyridinium derivative + ADP + NAD

2bov – CbExo + GDP + Ral-A GTPase
2a78 – CbExo catalytic domain + GDP + Ral-A GTPase
2a9k – CbExo catalytic domain+ GDP + Ral-A GTPase + NAD
2c8a – CbExo catalytic domain + nicotinamide
2c8c – CbExo catalytic domain (mutant) + ADP + NAD
2c8f, 2c8h – CbExo catalytic domain (mutant) + NAD

Exoenzyme S

1r4t – PaExo GAP domain – Pseudomonas aeruginosa - NMR
1he1 – PaExo GAP domain + Ras-like protein + GDP
1he9 – PaExo GAP domain + Ras-like protein

Exoenzyme T

4jmf – PaExo residues 28-77 + chaperone