3bw8

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spinqualitylabelsall models PDB ID 3bw8 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image
, resolution 1.80Å
Sites:
Ligands:
Gene:	c3 (Clostridium limosum)
Domains:	VIP2
Related:	1OJQ, 1OJZ, 1GZF, 1GZE
Resources:	FirstGlance, OCA, PDBsum, RCSB
Coordinates:	save as pdb, mmCIF, xml

Crystal structure of the Clostridium limosum C3 exoenzyme

Overview

C3-like toxins ADP-ribosylate and inactivate Rho GTPases. Seven C3-like ADP-ribosyltransferases produced by Clostridium botulinum, Clostridium limosum, Bacillus cereus and Staphylococcus aureus were identified and two representatives - C3bot from C. botulinum and C3stau2 from S. aureus - were crystallized. Here we present the 1.8A structure of C. limosum C3 transferase C3lim and compare it to the structures of other family members. In contrast to the structure of apo-C3bot, the canonical ADP-ribosylating turn turn motif is observed in a primed conformation, ready for NAD binding. This suggests an impact on the binding mode of NAD and on the transferase reaction. The crystal structure explains why auto-ADP-ribosylation of C3lim at Arg41 interferes with the ADP-ribosyltransferase activity of the toxin.

About this Structure

3BW8 is a Single protein structure of sequence from Clostridium limosum. Full crystallographic information is available from OCA.

Reference

Crystal structure of the Clostridium limosum C3 exoenzyme., Vogelsgesang M, Stieglitz B, Herrmann C, Pautsch A, Aktories K, FEBS Lett. 2008 Apr 2;582(7):1032-6. Epub 2008 Mar 4. PMID:18325337

Page seeded by OCA on Wed Apr 2 11:59:45 2008