Johnson's Monday Lab Sandbox for Insulin Receptor

From Proteopedia

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spinqualitylabelsall models Insulin Receptor with Four Insulin Bound - 6sof Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image This is a default text for your page Johnson's Monday Lab Sandbox for Insulin Receptor. Click above on edit this page to modify. Be careful with the < and > signs. You may include any references to papers as in: the use of JSmol in Proteopedia [1] or to the article describing Jmol [2] to the rescue. Contents 1 Purpose of Receptor 2 Insulin 3 Structure 3.1 Conformational Changes 3.2 How Insulin Binds Purpose of Receptor Insulin Structure Conformational Changes How Insulin Binds The insulin receptor unit has four separate sites for the insulin molecule to bind to. There are two pairs of two identical binding sites referred to as 1 and 1' and then 2 and 2'. The insulin molecules bind to these sites mostly through hydrophobic interactions. Despite a majority of the interactions being similar, sites 1 and 1' have a higher binding affinity than sites 2 and 2' due to site one having a larger surface area (706 square angstroms) exposed for insulin to bind to compared to site 2 (394 square angstroms)[3]

References

^{[3] [4] [5]}

1. ↑ Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:http://dx.doi.org/10.1002/ijch.201300024
2. ↑ Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:21638687 doi:10.1002/bmb.2006.494034042644
3. ↑ ^{3.0 3.1} . It was found that at least three insulin molecules would have to bound to the receptor for the receptor to take on its active “T-state” conformation <ref>. The difference between have the fully bound state with four insulins and the three insulin bound state is minimal compared to the difference between two and three insulins bound <ref name=”Uchikawa”. [http://</li> <li id="cite_note-Boucher-3">[[#cite_ref-Boucher_3-0|↑]] Boucher J, Kleinridders A, Kahn CR. Insulin receptor signaling in normal and insulin-resistant states. Cold Spring Harb Perspect Biol. 2014 Jan 1;6(1). pii: 6/1/a009191. doi:, 10.1101/cshperspect.a009191. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/24384568 24384568] doi:[http://dx.doi.org/10.1101/cshperspect.a009191 http://dx.doi.org/10.1101/cshperspect.a009191]</li> <li id="cite_note-Meyts-4">[[#cite_ref-Meyts_4-0|↑]] De Meyts P. The structural basis of insulin and insulin-like growth factor-I receptor binding and negative co-operativity, and its relevance to mitogenic versus metabolic signalling. Diabetologia. 1994 Sep;37 Suppl 2:S135-48. doi: 10.1007/bf00400837. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7821729 7821729] doi:[http://dx.doi.org/10.1007/bf00400837 http://dx.doi.org/10.1007/bf00400837]</li></ol></ref>

Student Contributors

• Maxwell Todd
• Abby Hillan
• Andrew Scheel