Sandbox Reserved 1654

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This Sandbox is Reserved from 26/11/2020, through 26/11/2021 for use in the course "Structural Biology" taught by Bruno Kieffer at the University of Strasbourg, ESBS. This reservation includes Sandbox Reserved 1643 through Sandbox Reserved 1664.
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Contents

Cytoplasmic Polyadenylation Element-Binding Protein (CPEB)

Structure

All CPEB proteins have a similar structure :

  • A N-terminal region which is a regulatory region with phosphorylation and dephosphorylation sites. This region is variable in length and composition.
  • A C-terminal region, composed of 2 recognition patterns : RRMs domains and zinc finger domains.
    • Zinc finger patterns [1]

Caption for this structure

Drag the structure with the mouse to rotate

About 54 residues with 6 cysteines and 2 histidines involved in a bond with a zinc atom, conserved for all isoforms and species. The modification of one of the eight zinc ligands destabilize the connection to the mRNA. includes :

      • A (Rd turn, residues 515-520), which is stabilized by hydrogen bonds between amide and sulfure.
      • β-hairpin with (residues 525-527) and (residues 533-535) between which there is an helical turn stabilized by hydrogen bonds.
      • An (residues 538-545) which forms the second bridge between the two zinc-binding sites. The surface-exposed face of the helix has a potential for specific intermolecular interactions with nucleic acids or proteins.
      • A 310 (residues 550-552).
      • 2 zinc binding sites, the first one is composed of and the second is composed of .
    • RRMs patterns [2]


Function

Disease

References

  1. ↑ Merkel DJ, Wells SB, Hilburn BC, Elazzouzi F, Perez-Alvarado GC, Lee BM. The C-Terminal Region of Cytoplasmic Polyadenylation Element Binding Protein Is a ZZ Domain with Potential for Protein-Protein Interactions. J Mol Biol. 2013 Mar 13. pii: S0022-2836(13)00156-3. doi:, 10.1016/j.jmb.2013.03.009. PMID:23500490 doi:10.1016/j.jmb.2013.03.009
  2. ↑ Afroz T, Skrisovska L, Belloc E, Guillen-Boixet J, Mendez R, Allain FH. A fly trap mechanism provides sequence-specific RNA recognition by CPEB proteins. Genes Dev. 2014 Jul 1;28(13):1498-514. doi: 10.1101/gad.241133.114. PMID:24990967 doi:http://dx.doi.org/10.1101/gad.241133.114
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