Structural highlights
Function
[HS3S5_HUMAN] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site. Also generates GlcUA-GlcNS or IdoUA-GlcNS and IdoUA2S-GlcNH2. The substrate-specific O-sulfation generates an enzyme-modified heparan sulfate which acts as a binding receptor to Herpes simplex virus-1 (HSV-1) and permits its entry.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The biosynthesis of heparan sulfate (HS) involves an array of specialized sulfotransferases. Here, we present a study aimed at engineering the substrate specificity of different HS 3-O-sulfotransferase isoforms. Based on the crystal structures, we identified a pair of amino acid residues responsible for selecting the substrates. Mutations of these residues altered the substrate specificities. Our results demonstrate the feasibility of tailoring the specificity of sulfotransferases to modify HS with desired functions.
Engineering sulfotransferases to modify heparan sulfate.,Xu D, Moon AF, Song D, Pedersen LC, Liu J Nat Chem Biol. 2008 Mar;4(3):200-2. Epub 2008 Jan 27. PMID:18223645[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Xia G, Chen J, Tiwari V, Ju W, Li JP, Malmstrom A, Shukla D, Liu J. Heparan sulfate 3-O-sulfotransferase isoform 5 generates both an antithrombin-binding site and an entry receptor for herpes simplex virus, type 1. J Biol Chem. 2002 Oct 4;277(40):37912-9. Epub 2002 Jul 23. PMID:12138164 doi:http://dx.doi.org/10.1074/jbc.M204209200
- ↑ Xu D, Moon AF, Song D, Pedersen LC, Liu J. Engineering sulfotransferases to modify heparan sulfate. Nat Chem Biol. 2008 Mar;4(3):200-2. Epub 2008 Jan 27. PMID:18223645 doi:10.1038/nchembio.66
