Crystal structure of a hypothetical protein from Giardia lamblia
Dylan Beard, Seonna Bristol, Kayla Cosby, Amber Davis, Courtney Manning, Lionel Perry, Lauren Snapp, Arian Toy, Kayla Wheeler, Jeremy Young, Bart L Staker, David Dranow, Jan Abendroth, Sandhya Subramanian, Thomas E Edwards, Peter J Myler, and Oluwatoyin A. Asojo [1]
Molecular Tour
Giardiasis or traveler's diarrhea can be fatal to the young, elderly, or immunocompromised. The Seattle Structural Genomics Center for Infectious Disease (SSGCID) has determined the structures of potential drug targets from Giardia lamblia. Some of these proteins are hypothetical proteins that share < 30% sequence identity or less to proteins with known structure. These studies are part of ongoing studies to identify proteins that could be targeted for rational drug discovery for giardiasis.
FFigure 3 Structural and primary sequence alignment of GilaA.00312.a and structurally similar YjgF/YER057c/UK114 endoribonucleases. The secondary structure elements are as follows: α�helices are shown as large coils, 310 helices ae shown in small coils labeled , beta-strand are shown in arrows labeled β, and beta-turns are labeled TT. The identical residues are shown on a red background with conserved residues in red and conserved regions in blue boxes. Figure generated using Espript (Gouet et al., 2003, Gouet et al., 1999).
The atomic resolution structure of a 15.6 kDa protein was determined by molecular replacement. The structure has the 2-layer alpha-beta-sandwich topology observed in the prototypical endoribonucleases L-PSP with conserved allosteric active sites containing small molecules from the crystallization solution. This paper is an educational collaboration between Hampton University (an HBCU) and the Seattle Structural Genomics Center for Infectious Disease (SSGCID).
References
- ↑ Beard DK, Bristol S, Cosby K, Davis A, Manning C, Perry L, Snapp L, Toy A, Wheeler K, Young J, Staker B, Arakaki TL, Abendroth J, Subrahamanian S, Edwards TE, Myler PJ, Asojo OA. Crystal structure of a hypothetical protein from Giardia lamblia. Acta Crystallogr F Struct Biol Commun. 2022 Feb 1;78(Pt 2):59-65. doi:, 10.1107/S2053230X21013595. Epub 2022 Jan 28. PMID:35102894 doi:http://dx.doi.org/10.1107/S2053230X21013595
