Structural and functional investigation of the human snRNP assembly factor AAR2 in complex with the PRPF8 RNaseH domain
Marco Preussner, Karine F. Santos, Jonathan Alles, Christina Heroven, Florian Heyd, Markus C. Wahl, Gert Weber [1]
Molecular Tour
Most of the metazoan genes are interspersed with non-coding information, so called introns. A large macromolecular machinery in the nucleus, the spliceosome, removes these introns from pre-mRNA transcripts linking the coding exons, to yield functional genes for translation.
. In this and the following scenes: AAR2Δloop, orange; PRPF8RH, light blue; a flexible loop (labeled Ser3) of AAR2 connecting its two domains, which in AAR2Δloop was replaced by three serine residues and another smaller flexible loop between residues 313-321 are labeled. N- and C-termini as well as the β-finger module of PRPF8RH are labeled.
The structure and functional data of the human spliceosomal assembly factor Aar2 in complex with a core spliceosomal domain of the PRPF8 protein indicates a different function of human Aar2 in contrast to the yeast protein.
References
- ↑ Preussner M, Santos KF, Alles J, Heroven C, Heyd F, Wahl MC, Weber G. Structural and functional investigation of the human snRNP assembly factor AAR2 in complex with the RNase H-like domain of PRPF8. Acta Crystallogr D Struct Biol. 2022 Nov 1;78(Pt 11):1373-1383. doi:, 10.1107/S2059798322009755. Epub 2022 Oct 27. PMID:36322420 doi:http://dx.doi.org/10.1107/S2059798322009755
