9oa6
From Proteopedia
Cryo-EM structure of the Zdhhc5-GOLGA7 complex
Structural highlights
FunctionGOGA7_HUMAN May be involved in protein transport from Golgi to cell surface. The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS.[1] [2] Publication Abstract from PubMedSmall molecules serve as valuable tools for probing nonapoptotic cell death mechanisms. The small molecule caspase independent lethal 56 (CIL56) induces a unique form of nonapoptotic cancer cell death that is promoted by a complex formed between zDHHC palmitoyltransferase 5 (ZDHHC5) and an accessory protein, golgin A7 (GOLGA7, also known as GCP16). The structure and function of this complex in nonapoptotic cell death regulation remain poorly understood. Here, we use coimmunoprecipitation, functional assays, and cryogenic electron microscopy (cryo-EM) to elucidate the structure and function of the Zdhhc5-GOLGA7 complex. We identify key residues in both Zdhhc5 and GOLGA7 that are necessary for complex formation and to promote nonapoptotic cancer cell death in response to caspase independent lethal 56. These results provide new insights into the structure and function of a death-promoting protein complex. Functional dissection of the zDHHC palmitoyltransferase 5-golgin A7 palmitoylation complex.,Kahlson MA, Ritho J, Gomes JV, Wang H, Butterwick JA, Dixon SJ J Biol Chem. 2025 Sep 8;301(10):110694. doi: 10.1016/j.jbc.2025.110694. PMID:40930250[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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