User:Karl E. Zahn/Sandbox RB69 1/palm-domain

From Proteopedia

The two-metal ion mechanism employed during the pol catalytic cycle relies on coordination of divalent cations by conserved motifs in the palm domain of DNA pols ^[1][2]. The palm domain of RB69 DNA pol (residues 383-468, 573-729) contains such motifs (VIYGDTD) that are particularly appropriate for family B DNA pols. Interestingly, the Thr (622) residue in this sequence must obtain a high energy conformation in order to correctly orient the Asp residues. This property is evident in Ramachandran plots of RB69 DNA pol where T622 is an obvious out-layer.