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Resolvase
From Proteopedia
(New page: Crystal structure of yeast Holliday junction resolvase, 1kcf {{STRUCTURE_1kcf| PDB=1kcf | SIZE=300| SCENE= |right|CAPTION=yeast Holliday junction ...) |
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[[Image:1kcf.png|left|200px|thumb|Crystal structure of yeast Holliday junction resolvase, [[1kcf]]]] | [[Image:1kcf.png|left|200px|thumb|Crystal structure of yeast Holliday junction resolvase, [[1kcf]]]] | ||
| - | {{STRUCTURE_1kcf| PDB=1kcf | SIZE=300| SCENE= |right|CAPTION= | + | {{STRUCTURE_1kcf| PDB=1kcf | SIZE=300| SCENE= |right|CAPTION=Yeast Holliday junction resolvase, [[1kcf]] }} |
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| + | Resolvase/Recombinase | ||
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| + | Resolvase or recombinase (Rec) is a nuclease which is involved in DNA recombination. The Holliday junction resolvase (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase. Tyr-recombinases include Cre, FLP and lambda integrase (LamInt). Ser-recombinases (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase. RadA recombinase promotes DNA recombination. | ||
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| + | {{TOC limit|limit=2}} | ||
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| + | == Holliday junction resolvase == | ||
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| + | [[2wiw]], [[2wiz]], [[2wj0]] – AfHJR+DNA – ''Archaeoglobus fulgidus''<br /> | ||
| + | [[2wcw]], [[2wcz]] – AfHJR (mutant)<br /> | ||
| + | [[2h8c]] – EcHJR (mutant) +DNA – ''Escherichia coli''<br /> | ||
| + | [[1gdt]] - EcHJR +DNA<br /> | ||
| + | [[2h8e]] - EcHJR (mutant)<br /> | ||
| + | [[1q8r]], [[1hjr]] – EcHJR<br /> | ||
| + | [[1hjr]] – EcHJR RuvC<br /> | ||
| + | [[1zp7]] – HJR – ''Bacillus subtilis''<br /> | ||
| + | [[1ob8]], 1hh1 – SsHJR – ''Sulfolobus solfataricus''<br /> | ||
| + | [[2fco]] – HJR – ''Geobacillus kaustophilus''<br /> | ||
| + | [[1ipi]], [[1gef]] – HJR – ''Pyrococcus furiosus''<br /> | ||
| + | [[1kcf]] – HJR – fission yeast<br /> | ||
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| + | == Ser-recombinase == | ||
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| + | [[2rsl]] - EcGDR<br /> | ||
| + | [[1hx7]] – EcGDR N-terminal – NMR<br /> | ||
| + | [[1ght]], [[1res]], [[1ret]], [[1ght]] – EcGDR catalytic domain – NMR<br /> | ||
| + | [[1gdr]], [[1hx7]] - EcGDR catalytic domain<br /> | ||
| + | [[2gm4]], [[1zr2]], [[1zr4]], [[1gdt]] – EcGDR+DNA<br /> | ||
| + | [[2gm5]] – EcGDR (mutant)<br /> | ||
| + | [[3guv]] – site-specific recombinase – ''Streptococcus pneumoniae''<br /> | ||
| + | [[2r0q]] – S-rec+DNA – ''Staphylococcus aureus''<br /> | ||
| + | [[3lhf]] – SsS-rec<br /> | ||
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| + | == Tyr-recombinase == | ||
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| + | [[3mgv]], [[3c28]], [[3c29]], [[2hof]], [[2hoi]], [[1xns]], [[1xo0]], [[1pvp]], [[1pvq]], [[1pvr]], [[1nzb]], [[1ouq]], [[1q3u]], [[1q3v]], [[1ma7]], [[1kbu]], [[1drg]], [[1f44]], [[2crx]], [[3crx]], [[4crx]], [[5crx]], [[1crx]] – EpCre+DNA – Enterobacteria phage PI<br /> | ||
| + | [[3etl]], [[3ew9]], [[3ewa]], [[2i1q]] – MmRadA Rec+AMPPNP+ion – ''Methanococcus maripaludis''<br /> | ||
| + | [[2b21]] - MmRadA Rec+AMPPNP<br /> | ||
| + | [[2zub]], [[2zuc]], [[2zud]], [[2dfl]] – SsRadA<br /> | ||
| + | [[2cvf]], [[2cvh]] – RadB Rec – ''Thermococcus kodakarensis''<br /> | ||
| + | [[2f1h]], [[2fpl]], [[2fpm]] - MvRec+AMPPNP+K – ''Methanococcus voltae''<br /> | ||
| + | [[2f1i]] – MvRec+AMPPNP<br /> | ||
| + | [[2f1j]], [[2fpk]] - MvRec+ADP<br /> | ||
| + | [[1a0p]] – EcXerd Rec<br /> | ||
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| + | == Integrase == | ||
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| + | [[2a3v]] – Rec INTI4+DNA – ''Vibrio cholerae''<br /> | ||
| + | [[3bvp]] – Integrase N-terminal – Lactococcus phage<br /> | ||
| + | [[2oxo]] – LamInt binding domain – phage<br /> | ||
| + | [[1z19]] – EpLamInt core binding +catalytic domain+DNA<br /> | ||
| + | [[1z1b]], [[1z1g]], [[1p7d]] – EpLamInt+DNA<br /> | ||
| + | [[1kjk]] – EpLamInt N-terminal – NMR<br /> | ||
| + | [[1ae9]] – EpLamInt catalytic core<br /> | ||
| + | [[1p4e]], [[1m6x]] – yFLP (mutant)+DNA – yeast<br /> | ||
| + | [[1flo]] - yFLP +DNA<br /> | ||
| + | [[1aih]] – HP1 integrase catalytic domain - bacteriophage<br /> | ||
Revision as of 07:35, 29 July 2010
Resolvase/Recombinase
Resolvase or recombinase (Rec) is a nuclease which is involved in DNA recombination. The Holliday junction resolvase (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase. Tyr-recombinases include Cre, FLP and lambda integrase (LamInt). Ser-recombinases (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase. RadA recombinase promotes DNA recombination.
Holliday junction resolvase
2wiw, 2wiz, 2wj0 – AfHJR+DNA – Archaeoglobus fulgidus
2wcw, 2wcz – AfHJR (mutant)
2h8c – EcHJR (mutant) +DNA – Escherichia coli
1gdt - EcHJR +DNA
2h8e - EcHJR (mutant)
1q8r, 1hjr – EcHJR
1hjr – EcHJR RuvC
1zp7 – HJR – Bacillus subtilis
1ob8, 1hh1 – SsHJR – Sulfolobus solfataricus
2fco – HJR – Geobacillus kaustophilus
1ipi, 1gef – HJR – Pyrococcus furiosus
1kcf – HJR – fission yeast
Ser-recombinase
2rsl - EcGDR
1hx7 – EcGDR N-terminal – NMR
1ght, 1res, 1ret, 1ght – EcGDR catalytic domain – NMR
1gdr, 1hx7 - EcGDR catalytic domain
2gm4, 1zr2, 1zr4, 1gdt – EcGDR+DNA
2gm5 – EcGDR (mutant)
3guv – site-specific recombinase – Streptococcus pneumoniae
2r0q – S-rec+DNA – Staphylococcus aureus
3lhf – SsS-rec
Tyr-recombinase
3mgv, 3c28, 3c29, 2hof, 2hoi, 1xns, 1xo0, 1pvp, 1pvq, 1pvr, 1nzb, 1ouq, 1q3u, 1q3v, 1ma7, 1kbu, 1drg, 1f44, 2crx, 3crx, 4crx, 5crx, 1crx – EpCre+DNA – Enterobacteria phage PI
3etl, 3ew9, 3ewa, 2i1q – MmRadA Rec+AMPPNP+ion – Methanococcus maripaludis
2b21 - MmRadA Rec+AMPPNP
2zub, 2zuc, 2zud, 2dfl – SsRadA
2cvf, 2cvh – RadB Rec – Thermococcus kodakarensis
2f1h, 2fpl, 2fpm - MvRec+AMPPNP+K – Methanococcus voltae
2f1i – MvRec+AMPPNP
2f1j, 2fpk - MvRec+ADP
1a0p – EcXerd Rec
Integrase
2a3v – Rec INTI4+DNA – Vibrio cholerae
3bvp – Integrase N-terminal – Lactococcus phage
2oxo – LamInt binding domain – phage
1z19 – EpLamInt core binding +catalytic domain+DNA
1z1b, 1z1g, 1p7d – EpLamInt+DNA
1kjk – EpLamInt N-terminal – NMR
1ae9 – EpLamInt catalytic core
1p4e, 1m6x – yFLP (mutant)+DNA – yeast
1flo - yFLP +DNA
1aih – HP1 integrase catalytic domain - bacteriophage
Proteopedia Page Contributors and Editors (what is this?)
Michal Harel, Alexander Berchansky, Joel L. Sussman, Jaime Prilusky
