Methyl CpG binding protein

Function

Methyl CpG binding proteins are called MBD1 to MBD4 and contain a methyl-CpG binding domain (MBD) which binds to methylated DNA.
• MBD1 is a transcription regulator. It reverts methylated cytosine bases to cytosine.
• MBD2 is part of the MeCP1 complex which contains also NuRD. MBD2 recruits histone deacetylases and DNA methyltransferases. MBD2 functions as a scaffold protein promoting repression.
• MBD3 binds to hydroxymethylated DNA.
• MBD4 has a G:T mipair-specific activity.
• Methyl CpG binding protein 2 (MeCP2) is present in mature nerve cells and is involved in turning off several genes. For more details see Methyl CpG Binding Protein 2.

Disease

Relevance

Structural highlights

3D structures of methyl CpG binding protein

Updated on 11-August-2014

Methyl CpG binding protein 1 (MBD1)

1ig4 – hMBD1 MBD domain + DNA – human – NMR
1d9n - hMBD1 MBD domain – NMR

Methyl CpG binding protein 2 (MBD2)

2l2l - hMBD2 coiled-coil domain + transcriptional repressor p66-α coiled-coil domain – NMR

Methyl CpG binding protein 3 (MBD3)

2mb7 – hMBD3 MBD domain – NMR

Methyl CpG binding protein 4 (MBD4)

1ngn - MBD4 glycosylate domain – mouse
3iho, 4e9e – hMBD4 glycosylate domain
4e9g – hMBD4 glycosylate domain + DNA
4dk9, 4e9f – hMBD4 glycosylate domain + DNA containing abasic dideoxyribose
4ew4 – mMBD4 glycosylate domain + DNA containing abasic dideoxyribose
4evv – mMBD4 glycosylate domain + DNA containing mismatch
4ew0 – mMBD4 glycosylate domain (mutant) + DNA containing hydroxymethyluracil
4e9h, 4ea4, 4ea5 – hMBD4 glycosylate domain (mutant) + DNA containing hydroxymethyluracil
3vxv, 3vxx, 3vyb, 3vyq – mMBD4 MBD + DNA containing cytidine derivative
4lg7 – hMBD4 MBD + DNA containing cytidine derivative

Methyl CpG binding protein 2 (MeCP2)

1qk9 - hMeCP2 MBD domain – NMR
3c2i – hMeCP2 MBD domain (mutant) + DNA
1ub1 – cMeCP2 attachment-region binding domain – chicken – NMR
2ky8 – cMBD4 MBD + DNA containing cytidine and uridine derivatives - NMR

References