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Glucanase

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{{STRUCTURE_3atg| PDB=3atg | SIZE=400| SCENE= |right| CAPTION=Endo-1,3-β-glucanase complex with glycerol, phosphate and Ca+2 ion, [[3atg]] }}
{{STRUCTURE_3atg| PDB=3atg | SIZE=400| SCENE= |right| CAPTION=Endo-1,3-β-glucanase complex with glycerol, phosphate and Ca+2 ion, [[3atg]] }}
-
'''Glucanases''' (Gase) are enzymes which break down glucans which are part of plant cell wall. Gase hydrolyzes the 1,4-β-glycosidic link in cellulose, lichenin and β-D-glucan. '''Endo-Gase''' breaks internal cellulose bonds. '''Exo-Gase''' cleaves 2 to 4 units from the ends of exposed cellulose polysaccharide chains. Gase are classified according to polysaccharide bond which they break. details in [[Molecular Playground/1,3-1,4-beta-D-glucanase]].
+
'''Glucanases''' (Gase) are enzymes which break down glucans which are part of plant cell wall. Gase hydrolyzes the 1,4-β-glycosidic link in cellulose, lichenin and β-D-glucan. '''Endo-Gase''' breaks internal cellulose bonds. '''Exo-Gase''' cleaves 2 to 4 units from the ends of exposed cellulose polysaccharide chains. '''Xyloglucanase''' (XGase) breaks down the hemicellulose xyloglucan. Gase are classified according to polysaccharide bond which they break. details in [[Molecular Playground/1,3-1,4-beta-D-glucanase]].
==3D structures of glucanase==
==3D structures of glucanase==
Line 20: Line 20:
**[[2cl2]] - Endo-Gase – ''Phanerochaete chrysosporium''<br />
**[[2cl2]] - Endo-Gase – ''Phanerochaete chrysosporium''<br />
**[[3ur7]], [[3ur8]] - pEndo-Gase – potato<br />
**[[3ur7]], [[3ur8]] - pEndo-Gase – potato<br />
-
**[[4bow]] - ZgEndo-Gase catalytic domain (mutant) – ''Zobellia galactanivorans''<br />
+
**[[4bow]], [[4crq]] - ZgEndo-Gase catalytic domain (mutant) – ''Zobellia galactanivorans''<br />
**[[4bq1]] - ZgEndo-Gase catalytic domain <br />
**[[4bq1]] - ZgEndo-Gase catalytic domain <br />
-
**[[4bpz]] - ZgEndo-Gase catalytic domain (mutant) + trisasccharide<br />
+
**[[4crr]] - ZgEndo-Gase C terminal <br />
 +
**[[4pq9]] - Endo-Gase – ''Mycobacterium marinum'' <br />
*''Endo-1,3– β-glucanase binary complex''
*''Endo-1,3– β-glucanase binary complex''
**[[4gzi]], [[4gzj]] - pEndo-Gase (mutant) + glucose<br />
**[[4gzi]], [[4gzj]] - pEndo-Gase (mutant) + glucose<br />
 +
**[[4bpz]] - ZgEndo-Gase catalytic domain (mutant) + trisasccharide<br />
*Endo-1,4-β-glucanase
*Endo-1,4-β-glucanase
Line 36: Line 38:
**[[3ik2]] - Endo-Gase A residues 34-541 – ''Clostridium acetobulylicum''<br />
**[[3ik2]] - Endo-Gase A residues 34-541 – ''Clostridium acetobulylicum''<br />
**[[2xfg]] - CtEndo-Gase 1 catalytic and cellulose-binding domains – ''Clostridium thermocellum''<br />
**[[2xfg]] - CtEndo-Gase 1 catalytic and cellulose-binding domains – ''Clostridium thermocellum''<br />
-
**[[2lro]], [[2lrp]] - CtEndo-Gase CBM11 domain – NMR<br />
 
-
**[[2v3g]], [[2cip]], [[2bv9]], [[4im4]] - CtEndo-Gase H catalytic domain<br />
 
**[[1cem]], [[2e0p]], [[1is9]] - CtEndo-Gase A catalytic domain<br />
**[[1cem]], [[2e0p]], [[1is9]] - CtEndo-Gase A catalytic domain<br />
**[[2wab]] - CtEndo-Gase E C terminal domain (mutant) <br />
**[[2wab]] - CtEndo-Gase E C terminal domain (mutant) <br />
-
**[[1v0a]] - CtEndo-Gase H<br />
 
**[[1ceo]] - CtEndo-Gase C (mutant) <br />
**[[1ceo]] - CtEndo-Gase C (mutant) <br />
**[[1cec]] - CtEndo-Gase C<br />
**[[1cec]] - CtEndo-Gase C<br />
-
**[[2eqd]], [[2cn3]], [[2jem]] - CtEndo-Gase (mutant) <br />
+
**[[1v0a]], [[4u3a]] - CtEndo-Gase H<br />
-
**[[2eo7]], [[2cn2]] - CtEndo-Gase<br />
+
**[[2v3g]], [[2cip]], [[2bv9]], [[4im4]] - CtEndo-Gase H catalytic domain<br />
-
**[[3qxf]] - EcEndo-Gase – Escherichia coli<br />
+
**[[2lro]], [[2lrp]] - CtEndo-Gase H CBM11 domain – NMR<br />
 +
**[[2eqd]] - CtEndo-Gase (mutant) <br />
 +
**[[2eo7]] - CtEndo-Gase<br />
 +
**[[3qxf]] - EcEndo-Gase – ''Escherichia coli<''br />
**[[3amh]], [[1vho]] - TmEndo-Gase –'' Thermotoga maritima''<br />
**[[3amh]], [[1vho]] - TmEndo-Gase –'' Thermotoga maritima''<br />
**[[3vhn]], [[3vho]], [[3vhp]] - TmEndo-Gase (mutant)<br />
**[[3vhn]], [[3vho]], [[3vhp]] - TmEndo-Gase (mutant)<br />
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**[[1ks8]], [[1ksc]], [[1ksd]] - Endo-Gase catalytic domain – termite<br />
**[[1ks8]], [[1ksc]], [[1ksd]] - Endo-Gase catalytic domain – termite<br />
**[[1h1n]] - Endo-Gase – ''Thermoascus aurantiacus''<br />
**[[1h1n]] - Endo-Gase – ''Thermoascus aurantiacus''<br />
-
**[[3a0f]] - Endo-Gase – ''Geotrichum''<br />
 
**[[3rjx]] - FnEndo-Gase – ''Fervidobacterium nodosum''<br />
**[[3rjx]] - FnEndo-Gase – ''Fervidobacterium nodosum''<br />
**[[3vl8]], [[3vl9]] - AaEndo-Gase – ''Aspergillus aculeatus''<br />
**[[3vl8]], [[3vl9]] - AaEndo-Gase – ''Aspergillus aculeatus''<br />
Line 81: Line 82:
**[[4h7m]] - CbEndo-Gase - ''Trichoderma harzianum''<br />
**[[4h7m]] - CbEndo-Gase - ''Trichoderma harzianum''<br />
**[[3wc3]] - Endo-Gase – ''Eisenia fetida''<br />
**[[3wc3]] - Endo-Gase – ''Eisenia fetida''<br />
 +
**[[4lx4]] - Endo-Gase – ''Pseudomonas stutzeri''<br />
 +
3fw6, 3ii1, [[4ee9]], [[4m24]] - ubEndo-Gase – uncultured bacterium<br />
 +
**[[4nf7]] - Endo-Gase – ''Butyrivibrio proteoclasticus''<br />
 +
**[[4q2b]] - Endo-Gase – ''Pseudomonas putida'' <br />
 +
**[[4v2x]] - BhEndo-Gase – ''Bacillus halodurans'' <br />
 +
**[[4uz8]], [[4uzn]] - BhEndo-Gase carbohydrate-binding module <br />
*''Endo-1,4–β-glucanase binary complex''
*''Endo-1,4–β-glucanase binary complex''
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**[[1k72]], [[1kfg]] - CcEndo-Gase 9G + polysaccharide<br />
**[[1k72]], [[1kfg]] - CcEndo-Gase 9G + polysaccharide<br />
**[[2wao]] - CtEndo-Gase E C terminal domain + polysaccharide<br />
**[[2wao]] - CtEndo-Gase E C terminal domain + polysaccharide<br />
 +
**[[4cj0]], [[4cj1]] - CtEndo-Gase D + affitin<br />
**[[2vi0]], [[2cit]] - CtEndo-Gase H catalytic domain (mutant) + polysaccharide<br />
**[[2vi0]], [[2cit]] - CtEndo-Gase H catalytic domain (mutant) + polysaccharide<br />
**[[2bvd]] - CtEndo-Gase H catalytic domain + polysaccharide<br />
**[[2bvd]] - CtEndo-Gase H catalytic domain + polysaccharide<br />
-
**[[1cen]] - CtEndo-Gase catalytic domain (mutant) + polysaccharide<br />
+
**[[1cen]], [[4u5i]], [[4u5k]] - CtEndo-Gase catalytic domain (mutant) + polysaccharide<br />
**[[3qxq]], [[2jen]] - EcEndo-Gase (mutant) + polysaccharide<br />
**[[3qxq]], [[2jen]] - EcEndo-Gase (mutant) + polysaccharide<br />
**[[3amm]] - TmEndo-Gase + polysaccharide<br />
**[[3amm]] - TmEndo-Gase + polysaccharide<br />
Line 109: Line 117:
**[[3pzt]] - BsEndo-Gase catalytic domain + Mn<br />
**[[3pzt]] - BsEndo-Gase catalytic domain + Mn<br />
**[[3rjy]] – FnEndo-Gase + glucose<br />
**[[3rjy]] – FnEndo-Gase + glucose<br />
-
**[[3vlb]] - AaEndo-Gase + protein EDGP
+
**[[3vlb]] - AaEndo-Gase + protein EDGP<br />
 +
**[[3wdu]], [[3wdv]] - PtEndo-Gase + polysaccharide<br />
 +
**[[3wdx]], [[3wdy]] - PtEndo-Gase (mutant) + polysaccharide<br />
*Endo-1,3-1,4-β-glucanase
*Endo-1,3-1,4-β-glucanase
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**[[1ghr]], [[1ghs]] - Endo-Gase – ''Hordeum vulgare''<br />
**[[1ghr]], [[1ghs]] - Endo-Gase – ''Hordeum vulgare''<br />
**[[1mac]] – Gase – ''Paenibacillus macerans''<br />
**[[1mac]] – Gase – ''Paenibacillus macerans''<br />
 +
**[[3wdt]] – PtEndo-Gase – ''Paecilomyces thermophila''<br />
 +
**[[3wdw]] – PtEndo-Gase (mutant)<br />
*''Endo-1,3-1,4–β-glucanase binary complex''
*''Endo-1,3-1,4–β-glucanase binary complex''
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**[[1aiw]] - EchEndo-Gase Z cellulose-binding domain – NMR<BR />
**[[1aiw]] - EchEndo-Gase Z cellulose-binding domain – NMR<BR />
**[[3ovw]] - FoEndo-Gase I – ''Fusarium oxysporum''<br />
**[[3ovw]] - FoEndo-Gase I – ''Fusarium oxysporum''<br />
-
**[[2jep]] - PpEndo-Gase – ''Paenibacillus pabuli'' <br />
 
**[[2yih]], [[3zq9]] - PpoEndo-Gase – ''Paenibacillus polymyxa''<br />
**[[2yih]], [[3zq9]] - PpoEndo-Gase – ''Paenibacillus polymyxa''<br />
**[[2yjq]], [[2ykk]] - PpoEndo-Gase (mutant)<br />
**[[2yjq]], [[2ykk]] - PpoEndo-Gase (mutant)<br />
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**[[2uwb]] - NaEndo-Gase (mutant)<br />
**[[2uwb]] - NaEndo-Gase (mutant)<br />
**[[3ez8]] - Endo-Gase – ''Alicyclobacillus acidocaldarius''<br />
**[[3ez8]] - Endo-Gase – ''Alicyclobacillus acidocaldarius''<br />
-
**[[3fw6]], [[3ii1]], [[4ee9]] - Endo-Gase – uncultured bacterium<br />
 
**[[4afm]] - EucEndo-Gase Cel5a N terminal – ''Eubacterium cellulosolvens''<br />
**[[4afm]] - EucEndo-Gase Cel5a N terminal – ''Eubacterium cellulosolvens''<br />
**[[4ba6]] - EucEndo-Gase Cel5a C terminal<br />
**[[4ba6]] - EucEndo-Gase Cel5a C terminal<br />
Line 191: Line 201:
*Exo-1,3-β-glucanase
*Exo-1,3-β-glucanase
-
**[[3o6a]] - CaExo-Gase (mutant) – ''Candida albicans''<br />
+
**[[3o6a]], [[4m80]] - CaExo-Gase (mutant) – ''Candida albicans''<br />
**[[1eqp]], [[1cz1]] - CaExo-Gase<br />
**[[1eqp]], [[1cz1]] - CaExo-Gase<br />
**[[1ex1]] - bExo-Gase<br />
**[[1ex1]] - bExo-Gase<br />
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**[[1eqc]] - CaExo-Gase + inhibitor<br />
**[[1eqc]] - CaExo-Gase + inhibitor<br />
**[[3n9k]]– CaExo-Gase (mutant) + polysaccharide<br />
**[[3n9k]]– CaExo-Gase (mutant) + polysaccharide<br />
 +
**[[4m81]], [[4m82]] - CaExo-Gase (mutant) + glucopyranoside<br />
*Exo-1,4-β-glucanase
*Exo-1,4-β-glucanase
Line 230: Line 241:
**[[4le3]] – PaBgase – ''Podospora anserina''<br />
**[[4le3]] – PaBgase – ''Podospora anserina''<br />
**[[4le4]] – PaBgase + cellotriose<br />
**[[4le4]] – PaBgase + cellotriose<br />
 +
 +
*Xyloglucanase
 +
 +
**[[3zmr]] - XGase + polysaccharide – ''Bacteroides ovatus''<br />
 +
**[[4npr]] - XGase – ''Aspergillus niveus''<br />
 +
**[[2cn2]] - CtXGase <br />
 +
**[[2cn3]] - CtXGase (mutant)<br />
 +
**[[2jem]] - BlXGase (mutant)<br />
 +
**[[2jep]] - PpXGase – ''Paenibacillus pabuli'' <br />
 +
**[[3a0f]] - Endo-Gase – ''Geotrichum''<br />
 +
**[[4w84]] - ubXGase <br />
 +
 +
*Xyloglucanase binary complexes
 +
 +
**[[4w85]], [[4w86]] - ubXGase + glucose<br />
 +
**[[4w87]], [[4w88]] - ubXGase + xyloglucan<br />
 +
**[[4w89]] - ubXGase + polysaccharide<br />
 +
**[[2jeq]] - PpXGase + polysaccharide<br />
 +
}}
}}

Revision as of 09:05, 7 April 2015

Template:STRUCTURE 3atg

Glucanases (Gase) are enzymes which break down glucans which are part of plant cell wall. Gase hydrolyzes the 1,4-β-glycosidic link in cellulose, lichenin and β-D-glucan. Endo-Gase breaks internal cellulose bonds. Exo-Gase cleaves 2 to 4 units from the ends of exposed cellulose polysaccharide chains. Xyloglucanase (XGase) breaks down the hemicellulose xyloglucan. Gase are classified according to polysaccharide bond which they break. details in Molecular Playground/1,3-1,4-beta-D-glucanase.

3D structures of glucanase

Updated on 07-April-2015

3fw6, 3ii1, 4ee9, 4m24 - ubEndo-Gase – uncultured bacterium

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

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