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Glucanase

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<StructureSection load='3atg' size='340' side='right' caption='Endo-1,3-β-glucanase complex with glycerol, phosphate and Ca+2 ion, [[3atg]]' scene=''>
<StructureSection load='3atg' size='340' side='right' caption='Endo-1,3-β-glucanase complex with glycerol, phosphate and Ca+2 ion, [[3atg]]' scene=''>
'''Glucanases''' (Gase) are enzymes which break down glucans which are part of plant cell wall. Gase hydrolyzes the 1,4-β-glycosidic link in cellulose, lichenin and β-D-glucan. '''Endo-Gase''' breaks internal cellulose bonds<ref>PMID:5354264</ref>. '''Exo-Gase''' cleaves 2 to 4 units from the ends of exposed cellulose polysaccharide chains<ref>PMID:26457517</ref>. '''Xyloglucanase''' (XGase) breaks down the hemicellulose xyloglucan<ref>PMID:9098958</ref>. Gase are classified according to polysaccharide bond which they break. details in [[Molecular Playground/1,3-1,4-beta-D-glucanase]].
'''Glucanases''' (Gase) are enzymes which break down glucans which are part of plant cell wall. Gase hydrolyzes the 1,4-β-glycosidic link in cellulose, lichenin and β-D-glucan. '''Endo-Gase''' breaks internal cellulose bonds<ref>PMID:5354264</ref>. '''Exo-Gase''' cleaves 2 to 4 units from the ends of exposed cellulose polysaccharide chains<ref>PMID:26457517</ref>. '''Xyloglucanase''' (XGase) breaks down the hemicellulose xyloglucan<ref>PMID:9098958</ref>. Gase are classified according to polysaccharide bond which they break. details in [[Molecular Playground/1,3-1,4-beta-D-glucanase]].
 +
 +
==3D structures of glucanase==
 +
[[Glucanase 3D structures]]
 +
</StructureSection>
</StructureSection>
==3D structures of glucanase==
==3D structures of glucanase==
Line 11: Line 15:
**[[5gzh]] - Endo-Gase – ''Chitinophaga linensis''<br />
**[[5gzh]] - Endo-Gase – ''Chitinophaga linensis''<br />
 +
**[[6imu]] - TfEndo-Gase – ''Talaromyces funiculosum'' <br />
 +
**[[6imv]] - TfEndo-Gase + sophorose <br />
 +
**[[6imw]] - TfEndo-Gase (mutant) + 1,2–β-glucan <br />
*Endo-1,3-β-glucanase
*Endo-1,3-β-glucanase
Line 28: Line 35:
**[[5fui]] - ZgEndo-Gase C terminal <br />
**[[5fui]] - ZgEndo-Gase C terminal <br />
**[[4pq9]] - Endo-Gase – ''Mycobacterium marinum'' <br />
**[[4pq9]] - Endo-Gase – ''Mycobacterium marinum'' <br />
-
**[[5h9x]] - PbEndo-Gase – ''Paenibacillus barengoltzii'' <br />
+
**[[5h9x]], [[6a6c]] - PbEndo-Gase – ''Paenibacillus barengoltzii'' <br />
**[[4wzf]] - MtEndo-Gase – ''Mycobacterium tuberculosis''<br />
**[[4wzf]] - MtEndo-Gase – ''Mycobacterium tuberculosis''<br />
**[[5m5z]], [[5m60]] - Endo-Gase – ''Chaetomium thermophilum''<br />
**[[5m5z]], [[5m60]] - Endo-Gase – ''Chaetomium thermophilum''<br />
Line 61: Line 68:
**[[3amh]], [[1vho]] - TmEndo-Gase –'' Thermotoga maritima''<br />
**[[3amh]], [[1vho]] - TmEndo-Gase –'' Thermotoga maritima''<br />
**[[3vhn]], [[3vho]], [[3vhp]] - TmEndo-Gase (mutant)<br />
**[[3vhn]], [[3vho]], [[3vhp]] - TmEndo-Gase (mutant)<br />
-
**[[2qno]] - CcEndo-Gase F catalytic domain – ''Clostridium cellulolyticum''<br />
+
**[[2qno]] - CclEndo-Gase F catalytic domain – ''Clostridium cellulolyticum''<br />
-
**[[1edg]] - CcEndo-Gase A catalytic domain<br />
+
**[[1edg]] - CclEndo-Gase A catalytic domain<br />
**[[2v38]], [[1w3k]] - BaEndo-Gase 5A catalytic domain – ''Bacillus agaradhaerens''<br />
**[[2v38]], [[1w3k]] - BaEndo-Gase 5A catalytic domain – ''Bacillus agaradhaerens''<br />
**[[1qhz]] - BaEndo-Gase B catalytic domain<br />
**[[1qhz]] - BaEndo-Gase B catalytic domain<br />
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**[[1js4]], [[1tf4]] - TfEndo-Gase E4 catalytic domain<br />
**[[1js4]], [[1tf4]] - TfEndo-Gase E4 catalytic domain<br />
**[[1tml]] - TfEndo-Gase catalytic domain<br />
**[[1tml]] - TfEndo-Gase catalytic domain<br />
-
**[[1wc2]] - Endo-Gase – Mytilus edulis<br />
+
**[[1wc2]] - Endo-Gase – ''Mytilus edulis''<br />
-
**[[1uww]] - Endo-Gase – Bacillus akibai<br />
+
**[[1uww]] - Endo-Gase – ''Bacillus akibai''<br />
**[[1oji]], [[1ojj]], [[1ojk]] - Endo-Gase (mutant) – ''Humicola oryzae''<br />
**[[1oji]], [[1ojj]], [[1ojk]] - Endo-Gase (mutant) – ''Humicola oryzae''<br />
**[[1gu3]] - CfEndo-Gase C – ''Cellulomonas fimi''<br />
**[[1gu3]] - CfEndo-Gase C – ''Cellulomonas fimi''<br />
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**[[3vl8]], [[3vl9]], [[5gm3]] - AaEndo-Gase – ''Aspergillus aculeatus''<br />
**[[3vl8]], [[3vl9]], [[5gm3]] - AaEndo-Gase – ''Aspergillus aculeatus''<br />
**[[4dod]] - CbEndo-Gase GH9 module – ''Caldicellulosiruptor bescii''<br />
**[[4dod]] - CbEndo-Gase GH9 module – ''Caldicellulosiruptor bescii''<br />
-
**[[4doe]] - CbEndo-Gase GH9 module + ligand<br />
+
**[[4h7m]], [[5w0a]] - CbEndo-Gase - ''Trichoderma harzianum''<br />
-
**[[4h7m]] - CbEndo-Gase - ''Trichoderma harzianum''<br />
+
**[[3wc3]] - Endo-Gase – ''Eisenia fetida''<br />
**[[3wc3]] - Endo-Gase – ''Eisenia fetida''<br />
**[[4lx4]] - Endo-Gase – ''Pseudomonas stutzeri''<br />
**[[4lx4]] - Endo-Gase – ''Pseudomonas stutzeri''<br />
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**[[1f9d]], [[1fbw]] - CcEndo-Gase F catalytic domain (mutant) + polysaccharide<br />
**[[1f9d]], [[1fbw]] - CcEndo-Gase F catalytic domain (mutant) + polysaccharide<br />
**[[1f9o]], [[1fae]], [[1fbo]] - CcEndo-Gase F catalytic domain + polysaccharide<br />
**[[1f9o]], [[1fae]], [[1fbo]] - CcEndo-Gase F catalytic domain + polysaccharide<br />
-
**[[1k72]], [[1kfg]] - CcEndo-Gase 9G + polysaccharide<br />
+
**[[1k72]], [[1kfg]], [[1j84]] - CcEndo-Gase 9G + polysaccharide<br />
**[[2wao]] - CtEndo-Gase E C terminal domain + polysaccharide<br />
**[[2wao]] - CtEndo-Gase E C terminal domain + polysaccharide<br />
**[[4cj0]], [[4cj1]] - CtEndo-Gase D + affitin<br />
**[[4cj0]], [[4cj1]] - CtEndo-Gase D + affitin<br />
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**[[3amn]], [[3amp]], [[3amq]] - TmEndo-Gase (mutant) + polysaccharide<br />
**[[3amn]], [[3amp]], [[3amq]] - TmEndo-Gase (mutant) + polysaccharide<br />
**[[2bod]], [[2bof]], [[2bog]] - TfEndo-Gase E2 catalytic domain + polysaccharide<br />
**[[2bod]], [[2bof]], [[2bog]] - TfEndo-Gase E2 catalytic domain + polysaccharide<br />
 +
**[[3tf4]], [[4tf4]] - TfEndo-Gase E4 catalytic domain + polysaccharide<br />
**[[1w3l]], [[1ocq]], [[1h2j]], [[1h11]], [[1h5v]], [[1hf6]], [[1e5j]] - BaEndo-Gase 5A catalytic domain + polysaccharide<br />
**[[1w3l]], [[1ocq]], [[1h2j]], [[1h11]], [[1h5v]], [[1hf6]], [[1e5j]] - BaEndo-Gase 5A catalytic domain + polysaccharide<br />
**[[2v38]], [[1ocq]] - BaEndo-Gase 5A catalytic domain + drug<br />
**[[2v38]], [[1ocq]] - BaEndo-Gase 5A catalytic domain + drug<br />
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**[[3acg]], [[3ach]] - CjEndo-Gase 5A residues 560-752 + polysaccharide<br />
**[[3acg]], [[3ach]] - CjEndo-Gase 5A residues 560-752 + polysaccharide<br />
**[[1uu4]], [[1uu5]], [[1uu6]], [[1w2u]] - HgEndo-Gase 12A catalytic domain + polysaccharide<br />
**[[1uu4]], [[1uu5]], [[1uu6]], [[1w2u]] - HgEndo-Gase 12A catalytic domain + polysaccharide<br />
-
**[[1j84]] - CcEndo-Gase ENGF + polysaccharide <br />
 
-
**[[3tf4]], [[4tf4]] - TfEndo-Gase E4 catalytic domain + polysaccharide<br />
 
**[[1ece]] - Endo-Gase E1 catalytic domain + polysaccharide – ''Acidothermus cellulolyticus''<br />
**[[1ece]] - Endo-Gase E1 catalytic domain + polysaccharide – ''Acidothermus cellulolyticus''<br />
-
**[[2vn5]], [[2vn6]] - CcEndo-Gase A residues 410-475 + cohesin residues 277-427<br />
+
**[[2vn5]], [[2vn6]] - CclEndo-Gase A residues 410-475 + cohesin residues 277-427<br />
**[[3pzt]] - BsEndo-Gase catalytic domain + Mn<br />
**[[3pzt]] - BsEndo-Gase catalytic domain + Mn<br />
**[[3rjy]] – FnEndo-Gase + glucose<br />
**[[3rjy]] – FnEndo-Gase + glucose<br />
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**[[5i79]] - AnEndo-Gase (mutant) + polysaccharide<br />
**[[5i79]] - AnEndo-Gase (mutant) + polysaccharide<br />
**[[5xc4]], [[5xc8]], [[5xc9]], [[5xbx]] - AcEndo-Gase + cellobiose <br />
**[[5xc4]], [[5xc8]], [[5xc9]], [[5xbx]] - AcEndo-Gase + cellobiose <br />
 +
**[[4doe]] - CbEndo-Gase GH9 module + ligand<br />
*Endo-1,3-1,4-β-glucanase
*Endo-1,3-1,4-β-glucanase
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**[[2c4x]] - CtEndo-Gase PKD and CBM44 domains (mutant)<br />
**[[2c4x]] - CtEndo-Gase PKD and CBM44 domains (mutant)<br />
**[[3isx]], [[2fvg]], [[1vjz]], [[3amc]], [[3amd]] - TmEndo-Gase – ''Thermotoga maritima''<br />
**[[3isx]], [[2fvg]], [[1vjz]], [[3amc]], [[3amd]] - TmEndo-Gase – ''Thermotoga maritima''<br />
 +
**[[5ne6]] - TmEndo-Gase M <br />
 +
**[[5ne7]], [[5ne8]], [[5ne9]] - TmEndo-Gase M (mutant)<br />
**[[3o7o]], [[3amg]] - TmEndo-Gase (mutant)<br />
**[[3o7o]], [[3amg]] - TmEndo-Gase (mutant)<br />
**[[3mmu]], [[3mmw]] - Endo-Gase 5A<br />
**[[3mmu]], [[3mmw]] - Endo-Gase 5A<br />
Line 268: Line 276:
**[[3gd9]] - SmBGase + polysaccharide<br />
**[[3gd9]] - SmBGase + polysaccharide<br />
**[[1byh]] – BGase/AGase – synthetic<br />
**[[1byh]] – BGase/AGase – synthetic<br />
-
**[[4le3]] – PaBgase – ''Podospora anserina''<br />
+
**[[4le3]] – PaBGase – ''Podospora anserina''<br />
-
**[[4le4]] – PaBgase + cellotriose<br />
+
**[[4le4]] – PaBGase + cellotriose<br />
 +
 
 +
* β-glucanase
 +
 
 +
**[[6euf]], [[6euj]] – BtBGase – ''Bacterioides thetaiotamicron''<br />
 +
**[[6eug]] – BtBGase + galactoimidazole<br />
 +
**[[6euh]] – BtBGase + jirimycin derivative<br />
 +
**[[6eui]] – BtBGase + galactose<br />
 +
**[[3wvj]] – CtBGase <br />
*Xyloglucanase
*Xyloglucanase
-
**[[3zmr]] - XGase + polysaccharide – ''Bacteroides ovatus''<br />
+
**[[3zmr]] - BoXGase – ''Bacteroides ovatus''<br />
**[[4npr]] - XGase – ''Aspergillus niveus''<br />
**[[4npr]] - XGase – ''Aspergillus niveus''<br />
**[[2cn2]] - CtXGase <br />
**[[2cn2]] - CtXGase <br />
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**[[2jem]] - BlXGase (mutant)<br />
**[[2jem]] - BlXGase (mutant)<br />
**[[2jep]] - PpXGase – ''Paenibacillus pabuli'' <br />
**[[2jep]] - PpXGase – ''Paenibacillus pabuli'' <br />
-
**[[3a0f]] - Endo-Gase – ''Geotrichum''<br />
+
**[[3a0f]] - XGase – ''Geotrichum''<br />
**[[4w84]] - ubXGase <br />
**[[4w84]] - ubXGase <br />
 +
**[[5fkt]] - XGase – ''Cellvibrio japonicus''<br />
*Xyloglucanase binary complexes
*Xyloglucanase binary complexes
Line 288: Line 305:
**[[4w89]] - ubXGase + polysaccharide<br />
**[[4w89]] - ubXGase + polysaccharide<br />
**[[2jeq]] - PpXGase + polysaccharide<br />
**[[2jeq]] - PpXGase + polysaccharide<br />
-
 
+
**[[3zmr]] - BoXGase + polysaccharide <br />
}}
}}
== References ==
== References ==

Revision as of 09:04, 10 July 2019

Endo-1,3-β-glucanase complex with glycerol, phosphate and Ca+2 ion, 3atg

Drag the structure with the mouse to rotate

3D structures of glucanase

Updated on 10-July-2019

References

  1. ↑ Barras DR, Stone BA. Beta-1,3-glucan hydrolases from Euglena gracilis. I. The nature of the hydrolases. Biochim Biophys Acta. 1969 Nov 4;191(2):329-41. PMID:5354264
  2. ↑ Tsai LC, Amiraslanov I, Chen HR, Chen YW, Lee HL, Liang PH, Liaw YC. Structures of exoglucanase from Clostridium cellulovorans: cellotetraose binding and cleavage. Acta Crystallogr F Struct Biol Commun. 2015 Oct 1;71(Pt 10):1264-72. doi:, 10.1107/S2053230X15015915. Epub 2015 Sep 23. PMID:26457517 doi:http://dx.doi.org/10.1107/S2053230X15015915
  3. ↑ Vincken JP, Beldman G, Voragen AG. Substrate specificity of endoglucanases: what determines xyloglucanase activity? Carbohydr Res. 1997 Mar 13;298(4):299-310. PMID:9098958

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Michal Harel, Alexander Berchansky, Joel L. Sussman

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