Glucanase

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Glucanases (Gase) are enzymes which break down glucans which are part of plant cell wall. Gase hydrolyzes the 1,4-β-glycosidic link in cellulose, lichenin and β-D-glucan. Endo-Gase breaks internal cellulose bonds[1]. Exo-Gase cleaves 2 to 4 units from the ends of exposed cellulose polysaccharide chains[2]. Xyloglucanase (XGase) breaks down the hemicellulose xyloglucan[3]. Gase are classified according to polysaccharide bond which they break. details in Molecular Playground/1,3-1,4-beta-D-glucanase.

3D structures of glucanase

Updated on 06-March-2016

3fw6, 3ii1, 4ee9, 4m24 - ubEndo-Gase – uncultured bacterium

References

  1. ↑ Barras DR, Stone BA. Beta-1,3-glucan hydrolases from Euglena gracilis. I. The nature of the hydrolases. Biochim Biophys Acta. 1969 Nov 4;191(2):329-41. PMID:5354264
  2. ↑ Tsai LC, Amiraslanov I, Chen HR, Chen YW, Lee HL, Liang PH, Liaw YC. Structures of exoglucanase from Clostridium cellulovorans: cellotetraose binding and cleavage. Acta Crystallogr F Struct Biol Commun. 2015 Oct 1;71(Pt 10):1264-72. doi:, 10.1107/S2053230X15015915. Epub 2015 Sep 23. PMID:26457517 doi:http://dx.doi.org/10.1107/S2053230X15015915
  3. ↑ Vincken JP, Beldman G, Voragen AG. Substrate specificity of endoglucanases: what determines xyloglucanase activity? Carbohydr Res. 1997 Mar 13;298(4):299-310. PMID:9098958

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