Resolvase

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Template:STRUCTURE 1kcf Resolvase or recombinase (Rec) is a nuclease which is involved in DNA recombination. The Holliday junction resolvase (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase. Tyr-recombinases include Cre which recombines loxP sites, FLP and lambda integrase (LamInt). Ser-recombinases (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase. RadA recombinase promotes DNA recombination. The images at the left and at the right correspond to one representative resolvase structure, i.e. the crystal structure of Ydc2 (also known as SpCce1), a Holliday junction resolvase from the fission yeast Schizosaccharomyces pombe that is involved in the maintenance of mitochondrial DNA (1kcf). Two sulfate ions are shown as space filling objects. Detailed analysis of its suggests mechanisms for junction isomerization and communication between the two active sites ^[1].

Contents 1 3D structures of resolvase 1.1 Holliday junction resolvase 1.2 Ser-recombinase 1.3 Tyr-recombinase 1.4 Cre recombinase 1.5 Integrase 1.6 Recombinase A (RecA) 2 References

3D structures of resolvase

Updated on 27-July-2014

Holliday junction resolvase

2wiw, 2wiz, 2wj0 – AfHJR+DNA – Archaeoglobus fulgidus
2wcw, 2wcz – AfHJR (mutant)
2h8c – EcHJR (mutant) +DNA – Escherichia coli
1gdt - EcHJR +DNA
2h8e - EcHJR (mutant)
1q8r, 1hjr – EcHJR
1hjr – EcHJR RuvC
1zp7 – HJR – Bacillus subtilis
1ob8, 1ob9, 1hh1 – SsHJR – Sulfolobus solfataricus
2fco – HJR – Geobacillus kaustophilus
1ipi, 1gef – HJR – Pyrococcus furiosus
1kcf – HJR – fission yeast

Ser-recombinase

2rsl - EcGDR
1hx7 – EcGDR N-terminal – NMR
1ght, 1res, 1ret, 1ght – EcGDR catalytic domain – NMR
1gdr, 1hx7 - EcGDR catalytic domain
2gm4, 1zr2, 1zr4, 1gdt – EcGDR+DNA
2gm5 – EcGDR (mutant)
3guv – site-specific recombinase – Streptococcus pneumoniae
2r0q – S-rec+DNA – Staphylococcus aureus
3lhf – SsS-rec

Tyr-recombinase

3etl, 3ew9, 3ewa, 2i1q – MmRadA Rec+AMPPNP+ion – Methanococcus maripaludis
2b21 - MmRadA Rec+AMPPNP
2zub, 2zuc, 2zud, 2dfl – SsRadA
2cvf, 2cvh – RadB Rec – Thermococcus kodakarensis
2f1h, 2fpl, 2fpm - MvRec+AMPPNP+K – Methanococcus voltae
2f1i – MvRec+AMPPNP
2f1j, 2fpk - MvRec+ADP
1a0p – EcXerd Rec

Cre recombinase

3mgv, 3c28, 3c29, 2hof, 2hoi, 1xns, 1xo0, 1pvp, 1pvq, 1pvr, 1nzb, 1ouq, 1q3u, 1q3v, 1ma7, 1kbu, 1drg, 1f44, 2crx, 3crx, 4crx, 5crx, 1crx – EpCre+DNA – Enterobacteria phage PI

Integrase

2a3v – Rec INTI4+DNA – Vibrio cholerae
3bvp – Integrase N-terminal – Lactococcus phage
2oxo – LamInt binding domain – phage
1z19 – EpLamInt core binding +catalytic domain+DNA
1z1b, 1z1g, 1p7d – EpLamInt+DNA
1kjk – EpLamInt N-terminal – NMR
1ae9 – EpLamInt catalytic core
1p4e, 1m6x – yFLP (mutant)+DNA – yeast
1flo - yFLP +DNA
1aih – HP1 integrase catalytic domain - bacteriophage

Recombinase A (RecA)

Recombinase A

References

1. ↑ Ceschini S, Keeley A, McAlister MS, Oram M, Phelan J, Pearl LH, Tsaneva IR, Barrett TE. Crystal structure of the fission yeast mitochondrial Holliday junction resolvase Ydc2. EMBO J. 2001 Dec 3;20(23):6601-11. PMID:11726496 doi:10.1093/emboj/20.23.6601