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FirstGlance/How to get average pLDDT from AlphaFold models

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Revision as of 23:33, 31 December 2025 by Eric Martz (Talk | contribs)
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It is easy to get the average confidence (pLDDT) for any range of residues in an AlphaFold model by using FirstGlance in Jmol. (Note that AlphaFold Database assigns the same pLDDT value to all atoms in an amino acid, while AlphaFold3 assigns different pLDDT values to each atom in an amino acid.)

  • Download the predicted structure as a .pdb file (or .cif file if .pdb is not available). (For the example below, residues 126-761 of human ubiquitin protein ligase E3A Q05086 were submitted to AlphaFold3. See case studies.)
  • If only the .cif file is available, convert it to .pdb format.
  • Go to FirstGlance in Jmol, and Upload the .pdb file.
  • After the model is displayed in FirstGlance, in the Views tab, click Find.. in the Focus Box:



  • Enter the range of sequence numbers in the Find.. slot. (Or enter multiple ranges separated by commas, for example 45-365, 380-682.)
  • Click List Found Atoms.
  • AlphaFold puts its predicted confidence (pLDDT) in the temperature column. The confidence/pLDDT statistics are at the bottom of the list as "temperature":

See Also

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