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Glucanase

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{{STRUCTURE_3amm| PDB=3amm | SIZE=400| SCENE= |right| CAPTION=Endoglucanase complex with cellotetraose, [[3amm]] }}
{{STRUCTURE_3amm| PDB=3amm | SIZE=400| SCENE= |right| CAPTION=Endoglucanase complex with cellotetraose, [[3amm]] }}
-
'''Glucanases''' (Gase) are enzymes which break down glucans which are part of plant cell wall. Gase hydrolyzes the 1,4-β-glycosidic link in cellulose, lichenin and β-D-glucan. Endo-Gase breaks internal cellulose bonds. Exo-Gase cleaves 2 to 4 units from the ends of exposed cellulose polysaccharide chains. Additional details in [[Molecular Playground/1,3-1,4-beta-D-glucanase]].
+
'''Glucanases''' (Gase) are enzymes which break down glucans which are part of plant cell wall. Gase hydrolyzes the 1,4-β-glycosidic link in cellulose, lichenin and β-D-glucan. Endo-Gase breaks internal cellulose bonds. Exo-Gase cleaves 2 to 4 units from the ends of exposed cellulose polysaccharide chains. Gase are classified according to polysaccharide bond which they break. details in [[Molecular Playground/1,3-1,4-beta-D-glucanase]].
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==3D structures of glucanase==
==3D structures of glucanase==
-
''Updated November 2011''
+
''Updated June 2012''
-
===Endo-glucanase===
+
===Endo-1,3-β-glucanase===
-
[[3o5s]], [[3pzu]], [[3pzv]]– Endo-Gase catalytic domain – ''Bacillus subtilis''<br />
+
[[3atg]] - Endo-Gase – Cellulosimicrobium cellulans<br />
-
[[3i4i]] - Endo-Gase residues 26-242 – ''Bacterium''<br />
+
[[3dgt]] - Endo-Gase catalytic domain – Streptomyces sioyaensis<br />
-
[[3d6e]] - Endo-Gase (mutant) – ''Bacillus licheniformis''<br />
+
[[3f55]], [[3em5]] - Endo-Gase – Hevea brasiliensis<br />
-
[[3h0o]], [[3hr9]], [[2r49]] - FsEndo-Gase (mutant) – ''Fibrobacter succinogenes''<br />
+
[[2vy0]] - Endo-Gase residues 35-297 – Pyrococcus furiosus<br />
-
[[1zm1]], [[1mve]] - FsEndo-Gase<br />
+
[[2hyk]] - Endo-Gase – Nococardiopsis<br />
-
[[3dgt]] - Endo-Gase catalytic domain – ''Streptomyces sioyaensis''<br />
+
[[2cyg]] - Endo-Gase – Musa acuminate<br />
-
[[3ndy]] - CcEndo-Gase D catalytic and cellulose-binding domains – ''Clostridium cellulovorans''<br />
+
[[4dfs]] - Endo-Gase catalytic domain – Thermotoga petrophila
-
[[1j83]] - CcEndo-Gase ENGF – ''Clostridium cellulovorans''<br />
+
 
-
[[3ik2]] - Endo-Gase A residues 34-541 – ''Clostridium acetobulylicum''<br />
+
===Endo-1,4-β-glucanase===
-
[[2xfg]] - CtEndo-Gase 1 catalytic and cellulose-binding domains – ''Clostridium thermocellum''<br />
+
 
 +
[[3pzu]], [[3pzv]] – BsEndo-Gase catalytic domain – Bacillus subtilis<br />
 +
[[2l8a]] - BsEndo-Gase CRM3 domain<br />
 +
[[3ndy]] - CcEndo-Gase D catalytic and cellulose-binding domains – Clostridium cellulovorans<br />
 +
[[1j83]] - CcEndo-Gase ENGF – Clostridium cellulovorans<br />
 +
[[3ik2]] - Endo-Gase A residues 34-541 – Clostridium acetobulylicum<br />
 +
[[2xfg]] - CtEndo-Gase 1 catalytic and cellulose-binding domains – Clostridium thermocellum<br />
[[2v3g]], [[2cip]], [[2bv9]] - CtEndo-Gase H catalytic domain<br />
[[2v3g]], [[2cip]], [[2bv9]] - CtEndo-Gase H catalytic domain<br />
[[1cem]], [[2e0p]], [[1is9]] - CtEndo-Gase A catalytic domain<br />
[[1cem]], [[2e0p]], [[1is9]] - CtEndo-Gase A catalytic domain<br />
Line 27: Line 33:
[[1ceo]] - CtEndo-Gase C (mutant) <br />
[[1ceo]] - CtEndo-Gase C (mutant) <br />
[[1cec]] - CtEndo-Gase C<br />
[[1cec]] - CtEndo-Gase C<br />
-
[[1clc]] - CtEndo-Gase D<br />
+
[[2eqd]], [[2cn3]], [[2jem]] - CtEndo-Gase (mutant) <br />
-
[[1l1y]], [[1l2a]] - CtEndo-Gase SS<br />
+
[[2eo7]], [[2cn2]] - CtEndo-Gase<br />
-
[[1daq]], [[1dav]] - CtEndo-Gase SS dockerin domain - NMR<br />
+
[[3qxf]] - EcEndo-Gase – Escherichia coli<br />
-
[[2eex]], [[2ej1]], [[2eqd]], [[2cn3]], [[2jem]] - CtEndo-Gase (mutant) <br />
+
[[3amh]] - TmEndo-Gase – Thermotoga maritima<br />
-
[[2eo7]], [[2c24]], [[2e4t]], [[2cn2]] - CtEndo-Gase<br />
+
[[2qno]] - CcEndo-Gase F catalytic domain – Clostridium cellulolyticum<br />
-
[[2c26]] - CtEndo-Gase PKD and CBM44 domains<br />
+
-
[[2c4x]] - CtEndo-Gase PKD and CBM44 domains (mutant)<br />
+
-
[[3qxf]] - EcEndo-Gase – ''Escherichia coli''<br />
+
-
[[3amh]], [[3isx]], [[2fvg]], [[1vjz]], [[3amc]], [[3amd]] - TmEndo-Gase – ''Thermotoga maritima''<br />
+
-
[[3o7o]], [[3amg]] - TmEndo-Gase (mutant) <br />
+
-
[[3mmu]], [[3mmw]] - Endo-Gase 5A<br />
+
-
[[2qno]] - CcEndo-Gase F catalytic domain – ''Clostridium cellulolyticum''<br />
+
[[1edg]] - CcEndo-Gase A catalytic domain<br />
[[1edg]] - CcEndo-Gase A catalytic domain<br />
-
[[1ga2]], [[1g87]] - CcEndo-Gase 9G<br />
+
[[2v38]], [[1w3k]] - BaEndo-Gase 5A catalytic domain – Bacillus agaradhaerens<br />
-
[[2v38]], [[1w3k]], [[8a3h]], [[7a3h]], [[1a3h]] - BaEndo-Gase 5A catalytic domain – ''Bacillus agaradhaerens''<br />
+
[[1qhz]] - BaEndo-Gase B catalytic domain
-
[[1qhz]] - BaEndo-Gase B catalytic domain <br />
+
[[2ckr]], [[2cks]] - TfEndo-Gase E5 catalytic domain - Thermobifida fusca
-
[[2ckr]], [[2cks]] - TfEndo-Gase E5 catalytic domain - ''Thermobifida fusca''<br />
+
[[2boe]] - TfEndo-Gase E2 catalytic domain (mutant) <br />
[[2boe]] - TfEndo-Gase E2 catalytic domain (mutant) <br />
 +
[[3prt]] - TfEndo-Gase E2 catalytic domain<br />
[[1js4]], [[1tf4]] - TfEndo-Gase E4 catalytic domain<br />
[[1js4]], [[1tf4]] - TfEndo-Gase E4 catalytic domain<br />
[[1tml]] - TfEndo-Gase catalytic domain<br />
[[1tml]] - TfEndo-Gase catalytic domain<br />
-
[[1wc2]] - Endo-Gase – ''Mytilus edulis''<br />
+
[[1wc2]] - Endo-Gase – Mytilus edulis<br />
-
[[1uww]] - Endo-Gase – ''Bacillus akibai''<br />
+
[[1uww]] - Endo-Gase – Bacillus akibai<br />
-
[[1oji]], [[1ojj]], [[1ojk]] - Endo-Gase (mutant) – ''Humicola oryzae''<br />
+
[[1oji]], [[1ojj]], [[1ojk]] - Endo-Gase (mutant) – Humicola oryzae<br />
-
[[1gu3]] - CfEndo-Gase C – ''Cellulomonas fim''i<br />
+
[[1gu3]] - CfEndo-Gase C – Cellulomonas fimi<br />
[[1ulo]], [[1cx1]] - CfEndo-Gase C cellulose-binding domain <br />
[[1ulo]], [[1cx1]] - CfEndo-Gase C cellulose-binding domain <br />
[[1ulp]] - CfEndo-Gase C cellulose-binding domain - NMR<BR />
[[1ulp]] - CfEndo-Gase C cellulose-binding domain - NMR<BR />
-
[[1dym]], [[1a39]] - HiEndo-Gase I (mutant) – ''Humicola insolens''<br />
+
[[1dym]], [[1a39]] - HiEndo-Gase I (mutant) – Humicola insolens<br />
-
[[2a39]] - HiEndo-Gase I<br />
+
[[1nlr]] - SlEndo-Gase catalytic domain – Streptomyces lividans<br />
-
[[2eng]] - HiEndo-Gase V<br />
+
[[1eg1]] - HjEndo-Gase catalytic domain (mutant) – Hypocrea jecorina<br />
-
[[1dys]], [[1hd5]] - HiEndo-Gase 6B catalytic domain<br />
+
-
[[1nlr]] - SlEndo-Gase catalytic domain – ''Streptomyces lividans''<br />
+
-
[[1aq0]] - bEndo-Gase – barley<br />
+
-
[[1eg1]] - HjEndo-Gase catalytic domain (mutant) – ''Hypocrea jecorina''<br />
+
[[3qr3]] - HjEndo-Gase catalytic domain<br />
[[3qr3]] - HjEndo-Gase catalytic domain<br />
-
[[1gbg]] - Endo-Gase – ''Bacillus licheniformis''<br />
+
[[3aci]], [[3acf]] - CjEndo-Gase 5A residues 560-752 – Clostridium josui<br />
-
[[1glh]] - Endo-Gase – synthetic<br />
+
[[1olq]], [[1oa2]], [[1oa3]], [[1oa4]] - TrEndo-Gase catalytic domain <br /> (mutant) – Trichoderma reesei<br />
-
[[1ghr]], [[1ghs]] - Endo-Gase – ''Hordeum vulgare''<br />
+
-
[[3aci]], [[3acf]] - CjEndo-Gase 5A residues 560-752 – ''Clostridium josui''<br />
+
-
[[3f55]], [[3em5]] - Endo-Gase – ''Hevea brasiliensis''<br />
+
-
[[2vy0]] - Endo-Gase residues 35-297 – ''Pyrococcus furiosus''<br />
+
-
[[2hyk]] - Endo-Gase – ''Nococardiopsis''<br />
+
-
[[2bw8]], [[1h0b]] - RmEndo-Gase 12A – ''Rhodothermus marinus''<br />
+
-
[[2cyg]] - Endo-Gase – ''Musa acuminata''<br />
+
-
[[1tvn]] - PhEndo-Gase G catalytic domain – ''Pseudoalteromonas haloplanktis''<br />
+
-
[[1olq]], [[1oa2]], [[1oa3]], [[1oa4]] - TrEndo-Gase catalytic domain (mutant) – ''Trichoderma reesei''<br />
+
[[1h8v]] - TrEndo-Gase 12A catalytic domain<br />
[[1h8v]] - TrEndo-Gase 12A catalytic domain<br />
-
[[1olr]] - HgEndo-Gase 12A catalytic domain – ''Humicola grisea''<br />
+
[[1olr]] - HgEndo-Gase 12A catalytic domain – Humicola grisea<br />
-
[[1oa9]], [[1l8f]] - MaEndo-Gase – ''Melanocarpus albomyces''<br />
+
[[1oa9]] - MaEndo-Gase – Melanocarpus albomyces<br />
-
[[1ks4]], [[1ks5]] - Endo-Gase A – ''Aspergillus niger''<br />
+
[[1ks8]], [[1ksc]], [[1ksd]] - Endo-Gase catalytic domain – termite<br />
[[1ks8]], [[1ksc]], [[1ksd]] - Endo-Gase catalytic domain – termite<br />
-
[[1h1n]], [[1gzj]] - Endo-Gase – ''Thermoascus aurantiacus''<br />
+
[[1h1n]] - Endo-Gase – Thermoascus aurantiacus<br />
-
[[1e8p]], [[1e8q]], [[2j4m]], [[2j4n]] - PeEndo-Gase cellulose docking domain – ''Piromyces equi'' – NMR<BR />
+
[[3a0f]] - Endo-Gase – Geotrichum<br />
-
[[1g01]] - Endo-Gase catalytic domain – ''Bacillus''<br />
+
[[3rjx]] - FnEndo-Gase – Fervidobacterium nodosum<br />
-
[[1egz]] - EchEndo-Gase Z catalytic domain – ''Erwinia chrysanthemi''<br />
+
[[3vl8]], [[3vl9]] - AaEndo-Gase – Aspergillus aculeatus
 +
 
 +
===Endo-1, 3-1, 4-β-glucanase===
 +
 
 +
[[3o5s]] - BsEndo-Gase catalytic domain
 +
[[3i4i]] - Endo-Gase residues 26-242 – Bacterium
 +
[[3d6e]] - Endo-Gase (mutant) – Bacillus licheniformis
 +
[[3h0o]], [[3hr9]], [[2r49]], [[3axd]] - FsEndo-Gase (mutant) – Fibrobacter succinogenes
 +
[[1zm1]], [[1mve]] - FsEndo-Gase
 +
[[1aq0]] - bEndo-Gase – barley
 +
[[1gbg]] - Endo-Gase – Bacillus licheniformis
 +
[[1glh]] - Endo-Gase – synthetic
 +
[[1ghr]], [[1ghs]] - Endo-Gase – Hordeum vulgare
 +
[[1mac]] – Gase – Paenibacillus macerans<br />
 +
 
 +
===Non-specific endo-glucanase===
 +
 
 +
[[1clc]] - CtEndo-Gase D
 +
[[1l1y]], [[1l2a]] - CtEndo-Gase SS
 +
[[1daq]], [[1dav]] - CtEndo-Gase SS dockerin domain]] - NMR<BR />
 +
[[2eex]], [[2ej1]] - CtEndo-Gase (mutant)
 +
[[2c24]], [[2e4t]] - CtEndo-Gase
 +
[[2c26]] - CtEndo-Gase PKD and CBM44 domains
 +
[[2c4x]] - CtEndo-Gase PKD and CBM44 domains (mutant)
 +
[[3isx]], [[2fvg]], [[1vjz]], [[3amc]], [[3amd]] - TmEndo-Gase – Thermotoga maritima
 +
[[3o7o]], [[3amg]] - TmEndo-Gase (mutant)
 +
[[3mmu]], [[3mmw]] - Endo-Gase 5A
 +
[[1ga2]], [[1g87]] - CcEndo-Gase 9G
 +
[[8a3h]], [[7a3h]], [[1a3h]] - BaEndo-Gase 5A catalytic domain – Bacillus agaradhaerens
 +
[[2a39]] - HiEndo-Gase I
 +
[[2eng]] - HiEndo-Gase V
 +
[[1dys]], [[1hd5]] - HiEndo-Gase 6B catalytic domain
 +
[[2bw8]], [[1h0b]] - RmEndo-Gase 12A – Rhodothermus marinus
 +
[[1tvn]] - PhEndo-Gase G catalytic domain – Pseudoalteromonas haloplanktis
 +
[[1l8f]] - MaEndo-Gase – Melanocarpus albomyces
 +
[[1ks4]], [[1ks5]] - Endo-Gase A – Aspergillus niger
 +
[[1gzj]] - Endo-Gase – Thermoascus aurantiacus
 +
[[1e8p]], [[1e8q]], [[2j4m]], [[2j4n]] - PeEndo-Gase cellulose docking domain – Piromyces – NMR<BR />
 +
[[1g01]] - Endo-Gase catalytic domain – Bacillus
 +
[[1egz]] - EchEndo-Gase Z catalytic domain – Erwinia chrysanthemi
[[1aiw]] - EchEndo-Gase Z cellulose-binding domain – NMR<BR />
[[1aiw]] - EchEndo-Gase Z cellulose-binding domain – NMR<BR />
-
[[3ovw]] - FoEndo-Gase I – ''Fusarium oxysporum''<br >
+
[[3ovw]] - FoEndo-Gase I – Fusarium oxysporum
-
[[2jep]] - PpEndo-Gase – ''Paenibacillus pabuli'' <br />
+
[[2jep]] - PpEndo-Gase – Paenibacillus pabuli
-
[[2yih]], [[3zq9]] - PpoEndo-Gase – ''Paenibacillus polymyxa''<br />
+
[[2yih]], [[3zq9]] - PpoEndo-Gase – Paenibacillus polymyxa
-
[[2yjq]], [[2ykk]] - PpoEndo-Gase (mutant)<br />
+
[[2yjq]], [[2ykk]] - PpoEndo-Gase (mutant)
-
[[2zew]], [[2zex]] - TpEndo-Gase – ''Thermoanaerobacterium polysaccharolyticus''<br />
+
[[2zew]], [[2zex]] - TpEndo-Gase – Thermoanaerobacterium polysaccharolyticus
-
[[2zez]] - TpEndo-Gase carbohydrate-binding module 2<br />
+
[[2zez]] - TpEndo-Gase carbohydrate-binding module 2
-
[[3a0f]] - Endo-Gase – ''Geotrichum''<br />
+
[[3ayr]] - PrEndo-Gase – Piromyces rhizinflatus
-
[[3ayr]] - PrEndo-Gase – ''Piromyces rhizinflatus''<br />
+
[[2uwa]] - NaEndo-Gase – Nasturium
-
[[2uwa]] - NaEndo-Gase – Nasturium<br />
+
[[2uwb]] - NaEndo-Gase (mutant)
-
[[2uwb]] - NaEndo-Gase (mutant)<br />
+
[[3ez8]] - Endo-Gase – Alicyclobacillus acidocaldarius
-
[[3ez8]] - Endo-Gase – ''Alicyclobacillus acidocaldarius''<br />
+
[[3fw6]], [[3ii1]] - Endo-Gase – uncultured bacterium
[[3fw6]], [[3ii1]] - Endo-Gase – uncultured bacterium
-
===Endo-glucanase binary complex===
 
-
[[3ndz]] - CcEndo-Gase D catalytic and cellulose-binding domains + polysacchride<br />
+
===Endo-1,4 – β-glucanase binary complex===
-
[[1kfg]] - CcEndo-Gase G catalytic domain + polysacchride<br />
+
 
-
[[1f9d]], [[1fbw]] - CcEndo-Gase F catalytic domain (mutant) + polysacchride<br />
+
[[3ndz]] - CcEndo-Gase D catalytic and cellulose-binding domains + polysaccharide<br />
-
[[1f9o]], [[1fae]], [[1fbo]] - CcEndo-Gase F catalytic domain + polysacchride<br />
+
[[1kfg]] - CcEndo-Gase G catalytic domain + polysaccharide<br />
-
[[1k72]], [[1kfg]] - CcEndo-Gase 9G + polysacchride<br />
+
[[1f9d]], [[1fbw]] - CcEndo-Gase F catalytic domain (mutant) + polysaccharide<br />
-
[[2wao]] - CtEndo-Gase E C terminal domain + polysacchride<br />
+
[[1f9o]], [[1fae]], [[1fbo]] - CcEndo-Gase F catalytic domain + polysaccharide<br />
-
[[2vi0]], [[2cit]] - CtEndo-Gase H catalytic domain (mutant) + polysacchride<br />
+
[[1k72]], [[1kfg]] - CcEndo-Gase 9G + polysaccharide<br />
-
[[2bvd]] - CtEndo-Gase H catalytic domain + polysacchride<br />
+
[[2wao]] - CtEndo-Gase E C terminal domain + polysaccharide<br />
-
[[1cen]] - CtEndo-Gase catalytic domain (mutant) + polysacchride<br />
+
[[2vi0]], [[2cit]] - CtEndo-Gase H catalytic domain (mutant) + polysaccharide<br />
-
[[1kwf]] - CtEndo-Gase A catalytic domain (mutant) + polysacchride<br />
+
[[2bvd]] - CtEndo-Gase H catalytic domain + polysaccharide<br />
-
[[3qxq]], [[2jen]] - EcEndo-Gase (mutant) + polysacchride<br />
+
[[1cen]] - CtEndo-Gase catalytic domain (mutant) + polysaccharide<br />
-
[[3amm]] - TmEndo-Gase + polysacchride<br />
+
[[3qxq]], [[2jen]] - EcEndo-Gase (mutant) + polysaccharide<br />
-
[[3amn]], [[3amp]], [[3amq]], [[3aof]], [[3azr]], [[3azs]], [[3azt]] - TmEndo-Gase (mutant) + polysacchride<br />
+
[[3amm]] - TmEndo-Gase + polysaccharide<br />
-
[[2bod]], [[2bof]], [[2bog]] - TfEndo-Gase E2 catalytic domain + polysacchride<br />
+
[[3amn]], [[3amp]], [[3amq]] - TmEndo-Gase (mutant) + polysaccharide<br />
-
[[1w3l]], [[1ocq]], [[1h2j]], [[1h11]], [[1h5v]], [[1hf6]], [[1e5j]] - BaEndo-Gase 5A catalytic domain + polysacchride<br />
+
[[2bod]], [[2bof]], [[2bog]] - TfEndo-Gase E2 catalytic domain + polysaccharide<br />
-
[[2v38]], [[1ocq]] - BaEndo-Gase 5A catalytic domain + drug<br />
+
[[1w3l]], [[1ocq]], [[1h2j]], [[1h11]], [[1h5v]], [[1hf6]], [[1e5j]] - BaEndo-Gase 5A catalytic domain + polysaccharide<br />
-
[[5a3h]], [[6a3h]], [[4a3h]], [[2a3h]], [[3a3h]] - BaEndo-Gase 5A catalytic domain + polysacchride<br />
+
[[2v38]], [[1ocq]] - BaEndo-Gase 5A catalytic domain + drug
-
[[1qi0]], [[1qi2]] - BaEndo-Gase B catalytic domain + polysacchride<br />
+
[[1qi0]], [[1qi2]] - BaEndo-Gase B catalytic domain + polysaccharide<br />
-
[[1u0a]] - Endo-Gase (mutant) + polysacchride – ''Paenibacillus macerans''<br />
+
[[1u0a]] - Endo-Gase (mutant) + polysaccharide<br /> – Paenibacillus macerans<br />
-
[[3acg]], [[3ach]] - CjEndo-Gase 5A residues 560-752 + polysacchride<br />
+
[[3acg]], [[3ach]] - CjEndo-Gase 5A residues 560-752 + polysaccharide<br />
-
[[2bwa]], [[2bwc]] - RmEndo-Gase 12A + polysacchride<br />
+
[[1uu4]], [[1uu5]], [[1uu6]], [[1w2u]] - HgEndo-Gase 12A catalytic domain + polysaccharide<br />
-
[[1tvp]] - PhEndo-Gase G catalytic domain + polysacchride<br />
+
[[1j84]] - CcEndo-Gase ENGF + polysaccharide<br /> – Clostridium cellulovorans<br />
-
[[1uu4]], [[1uu5]], [[1uu6]], [[1w2u]] - HgEndo-Gase 12A catalytic domain + polysacchride<br />
+
[[3tf4]], [[4tf4]] - TfEndo-Gase E4 catalytic domain + polysaccharide<br />
-
[[1oa7]] - MaEndo-Gase + polysacchride<br />
+
[[1ece]] - Endo-Gase E1 catalytic domain + polysaccharide<br /> – Acidothermus cellulolyticus
-
[[1j84]] - CcEndo-Gase ENGF + polysacchride – ''Clostridium cellulovorans''<br />
+
[[2vn5]], [[2vn6]] - CcEndo-Gase A residues 410-475 + cohesin residues 277-427
-
[[1g0c]] - Endo-Gase catalytic domain + polysacchride – ''Bacillus''<br />
+
[[3pzt]] - BsEndo-Gase catalytic domain + Mn
-
[[2nlr]] - SlEndo-Gase catalytic domain + polysacchride<br />
+
[[3rjy]] – FnEndo-Gase + glucose
-
[[2ovw]], [[4ovw]], [[1ovw]] - FoEndo-Gase I + polysacchride<br />
+
[[3vlb]] - AaEndo-Gase + protein EDGP
-
[[3tf4]], [[4tf4]] - TfEndo-Gase E4 catalytic domain + polysacchride<br />
+
 
-
[[3eng]], [[4eng]] - HiEndo-Gase V catalytic domain + polysacchride<br />
+
===Endo-1,3-1,4–β-glucanase binary complex===
-
[[1ece]] - Endo-Gase E1 catalytic domain + polysacchride – ''Acidothermus cellulolyticus''<br />
+
 
-
[[2vn5]], [[2vn6]] - CcEndo-Gase A residues 410-475 + cohesin residues 277-427<br />
+
[[3axe]] - FsEndo-Gase (mutant) + polysaccharide<br />
-
[[1uoz]], [[1up0]], [[1up2]], [[1up3]] - Endo-Gase + polysaccharide – ''Mycobacterium tuberculosis''<br />
+
 
 +
===Non-specific endo-glucanase binary complex===
 +
 
 +
[[1kwf]] - CtEndo-Gase A catalytic domain (mutant) + polysaccharide<br />
 +
[[3aof]], [[3azr]], [[3azs]], [[3azt]] - TmEndo-Gase (mutant) + polysaccharide<br />
 +
[[5a3h]], [[6a3h]], [[4a3h]], [[2a3h]], [[3a3h]] - BaEndo-Gase 5A catalytic domain + polysaccharide<br />
 +
[[2bwa]], [[2bwc]] - RmEndo-Gase 12A + polysaccharide<br />
 +
[[1tvp]] - PhEndo-Gase G catalytic domain + polysaccharide<br />
 +
[[1oa7]] - MaEndo-Gase + polysaccharide<br />
 +
[[1g0c]] - Endo-Gase catalytic domain + polysaccharide – Bacillus<br />
 +
[[2nlr]] - SlEndo-Gase catalytic domain + polysaccharide<br />
 +
[[2ovw]], [[4ovw]], [[1ovw]] - FoEndo-Gase I + polysaccharide<br />
 +
[[3eng]], [[4eng]] - HiEndo-Gase V catalytic domain + polysaccharide<br />
 +
[[1uoz]], [[1up0]], [[1up2]], [[1up3]] - Endo-Gase + polysaccharide – Mycobacterium tuberculosis<br />
[[2jeq]] - PpEndo-Gase + polysaccharide<br />
[[2jeq]] - PpEndo-Gase + polysaccharide<br />
[[2zey]] - TpEndo-Gase + polysaccharide<br />
[[2zey]] - TpEndo-Gase + polysaccharide<br />
[[3ays]] - PrEndo-Gase (mutant) + polysaccharide<br />
[[3ays]] - PrEndo-Gase (mutant) + polysaccharide<br />
-
[[3oea]], [[3oeb]] - Endo-Gase (mutant) + polysaccharide – ''Caldanaerobius polysaccharolyticus''<br />
+
[[3oea]], [[3oeb]] - Endo-Gase (mutant) + polysaccharide – Caldanaerobius polysaccharolyticus<br />
-
[[3nco]] - Endo-Gase + peptide – ''Fervidobacterium nodosum''<br />
+
[[3nco]] - FnEndo-Gase + peptide<br />
-
[[3pzt]] - BsEndo-Gase catalytic domain + Mn
+
-
 
+
-
===Exo-glucanase===
+
===Exo-1,3-β-glucanase===
-
[[3o6a]] - CaExo-Gase (mutant) – ''Candida albicans''<br />
+
[[3o6a]] - CaExo-Gase (mutant) – Candida albicans<br />
[[1eqp]], [[1cz1]] - CaExo-Gase<br />
[[1eqp]], [[1cz1]] - CaExo-Gase<br />
[[1ex1]] - bExo-Gase<br />
[[1ex1]] - bExo-Gase<br />
 +
[[1h4p]] - Exo-Gase – yeast
 +
 +
===Exo-1,4-β-glucanase===
 +
[[1ut9]] - CtExo-Gase catalytic domain<br />
[[1ut9]] - CtExo-Gase catalytic domain<br />
[[1q2b]], [[1egn]], [[2cel]], [[4cel]] - HjExo-Gase I catalytic domain (mutant) <br />
[[1q2b]], [[1egn]], [[2cel]], [[4cel]] - HjExo-Gase I catalytic domain (mutant) <br />
-
[[1h4p]] - Exo-Gase – yeast<br />
+
[[1h46]], [[1gpi]] - PcExo-Gase I catalytic domain – Phanerochaete chrysosporium
-
[[1h46]], [[1gpi]] - PcExo-Gase I catalytic domain – ''Phanerochaete chrysosporium''
+
 
 +
=== Exo-1,3-1,4-β-glucanase===
 +
 
 +
[[3f95]] - PsExo-Gase C terminal domain – Pseudoalteromonas<br />
 +
[[3rrx]] - PsExo-Gase (mutant) <br />
 +
[[3usz]], [[3ut0]] - PsExo-Gase<br />
-
===Exo-glucanase binary complex===
+
===Exo-1,3-β-glucanase binary complex===
[[1eqc]] - CaExo-Gase + inhibitor<br />
[[1eqc]] - CaExo-Gase + inhibitor<br />
-
[[3n9k]] – CaExo-Gase (mutant) + polysacchride<br />
+
[[3n9k]]– CaExo-Gase (mutant) + polysaccharide<br />
 +
 
 +
===Exo-1,4-β-glucanase binary complex===
 +
 
[[3cuf]], [[3cug]], [[3cuh]], [[3cui]], [[3cuj]] - CfExo-Gase + polysaccharide<br />
[[3cuf]], [[3cug]], [[3cuh]], [[3cui]], [[3cuj]] - CfExo-Gase + polysaccharide<br />
-
[[2v3i]], [[2v3r]] - HjExo-Gase I catalytic domain + phenanthrenolol<br />
+
[[2v3i]], [[2v3r]] - HjExo-Gase I catalytic domain + phenanthrenolol <br />
-
[[1q2e]], [[5cel]], [[6cel]], [[7cel]], [[3cel]] - HjExo-Gase I catalytic domain (mutant) + polysacchride<br />
+
[[1q2e]], [[5cel]], [[6cel]], [[7cel]], [[3cel]] - HjExo-Gase I catalytic domain (mutant) + polysaccharide<br />
[[1dy4]] - TrExo-Gase I catalytic domain + propranolol
[[1dy4]] - TrExo-Gase I catalytic domain + propranolol
===β-1,3-glucanase (BGase)===
===β-1,3-glucanase (BGase)===
-
[[2jon]] – BGase C terminal – olive – NMR<br />
+
[[2jon]] – BGase C terminal – olive – NMR<BR />
[[3eqn]] – PcBGase<br />
[[3eqn]] – PcBGase<br />
[[3eqo]] – PcBGase + gluconolactone<br />
[[3eqo]] – PcBGase + gluconolactone<br />
-
[[3gdo]] – SmBGase – ''Streptomyces matensis''<br />
+
[[3gdo]] – SmBGase – Streptomyces matensis<br />
[[3gd9]] - SmBGase + polysaccharide<br />
[[3gd9]] - SmBGase + polysaccharide<br />
[[1byh]] – BGase/AGase – synthetic
[[1byh]] – BGase/AGase – synthetic
-
===Endo-1,3-1,4- β-glucanase===
 
- 
-
[[1mac]] – Gase – ''Paenibacillus macerans''
 

Revision as of 11:28, 11 June 2012

Template:STRUCTURE 3amm

Glucanases (Gase) are enzymes which break down glucans which are part of plant cell wall. Gase hydrolyzes the 1,4-β-glycosidic link in cellulose, lichenin and β-D-glucan. Endo-Gase breaks internal cellulose bonds. Exo-Gase cleaves 2 to 4 units from the ends of exposed cellulose polysaccharide chains. Gase are classified according to polysaccharide bond which they break. details in Molecular Playground/1,3-1,4-beta-D-glucanase.

Contents

3D structures of glucanase

Updated June 2012

Endo-1,3-β-glucanase

3atg - Endo-Gase – Cellulosimicrobium cellulans
3dgt - Endo-Gase catalytic domain – Streptomyces sioyaensis
3f55, 3em5 - Endo-Gase – Hevea brasiliensis
2vy0 - Endo-Gase residues 35-297 – Pyrococcus furiosus
2hyk - Endo-Gase – Nococardiopsis
2cyg - Endo-Gase – Musa acuminate
4dfs - Endo-Gase catalytic domain – Thermotoga petrophila

Endo-1,4-β-glucanase

3pzu, 3pzv – BsEndo-Gase catalytic domain – Bacillus subtilis
2l8a - BsEndo-Gase CRM3 domain
3ndy - CcEndo-Gase D catalytic and cellulose-binding domains – Clostridium cellulovorans
1j83 - CcEndo-Gase ENGF – Clostridium cellulovorans
3ik2 - Endo-Gase A residues 34-541 – Clostridium acetobulylicum
2xfg - CtEndo-Gase 1 catalytic and cellulose-binding domains – Clostridium thermocellum
2v3g, 2cip, 2bv9 - CtEndo-Gase H catalytic domain
1cem, 2e0p, 1is9 - CtEndo-Gase A catalytic domain
2wab - CtEndo-Gase E C terminal domain (mutant)
1v0a - CtEndo-Gase H
1ceo - CtEndo-Gase C (mutant)
1cec - CtEndo-Gase C
2eqd, 2cn3, 2jem - CtEndo-Gase (mutant)
2eo7, 2cn2 - CtEndo-Gase
3qxf - EcEndo-Gase – Escherichia coli
3amh - TmEndo-Gase – Thermotoga maritima
2qno - CcEndo-Gase F catalytic domain – Clostridium cellulolyticum
1edg - CcEndo-Gase A catalytic domain
2v38, 1w3k - BaEndo-Gase 5A catalytic domain – Bacillus agaradhaerens
1qhz - BaEndo-Gase B catalytic domain 2ckr, 2cks - TfEndo-Gase E5 catalytic domain - Thermobifida fusca 2boe - TfEndo-Gase E2 catalytic domain (mutant)
3prt - TfEndo-Gase E2 catalytic domain
1js4, 1tf4 - TfEndo-Gase E4 catalytic domain
1tml - TfEndo-Gase catalytic domain
1wc2 - Endo-Gase – Mytilus edulis
1uww - Endo-Gase – Bacillus akibai
1oji, 1ojj, 1ojk - Endo-Gase (mutant) – Humicola oryzae
1gu3 - CfEndo-Gase C – Cellulomonas fimi
1ulo, 1cx1 - CfEndo-Gase C cellulose-binding domain
1ulp - CfEndo-Gase C cellulose-binding domain - NMR
1dym, 1a39 - HiEndo-Gase I (mutant) – Humicola insolens
1nlr - SlEndo-Gase catalytic domain – Streptomyces lividans
1eg1 - HjEndo-Gase catalytic domain (mutant) – Hypocrea jecorina
3qr3 - HjEndo-Gase catalytic domain
3aci, 3acf - CjEndo-Gase 5A residues 560-752 – Clostridium josui
1olq, 1oa2, 1oa3, 1oa4 - TrEndo-Gase catalytic domain
(mutant) – Trichoderma reesei
1h8v - TrEndo-Gase 12A catalytic domain
1olr - HgEndo-Gase 12A catalytic domain – Humicola grisea
1oa9 - MaEndo-Gase – Melanocarpus albomyces
1ks8, 1ksc, 1ksd - Endo-Gase catalytic domain – termite
1h1n - Endo-Gase – Thermoascus aurantiacus
3a0f - Endo-Gase – Geotrichum
3rjx - FnEndo-Gase – Fervidobacterium nodosum
3vl8, 3vl9 - AaEndo-Gase – Aspergillus aculeatus

Endo-1, 3-1, 4-β-glucanase

3o5s - BsEndo-Gase catalytic domain 3i4i - Endo-Gase residues 26-242 – Bacterium 3d6e - Endo-Gase (mutant) – Bacillus licheniformis 3h0o, 3hr9, 2r49, 3axd - FsEndo-Gase (mutant) – Fibrobacter succinogenes 1zm1, 1mve - FsEndo-Gase 1aq0 - bEndo-Gase – barley 1gbg - Endo-Gase – Bacillus licheniformis 1glh - Endo-Gase – synthetic 1ghr, 1ghs - Endo-Gase – Hordeum vulgare 1mac – Gase – Paenibacillus macerans

Non-specific endo-glucanase

1clc - CtEndo-Gase D 1l1y, 1l2a - CtEndo-Gase SS 1daq, 1dav - CtEndo-Gase SS dockerin domain]] - NMR
2eex, 2ej1 - CtEndo-Gase (mutant) 2c24, 2e4t - CtEndo-Gase 2c26 - CtEndo-Gase PKD and CBM44 domains 2c4x - CtEndo-Gase PKD and CBM44 domains (mutant) 3isx, 2fvg, 1vjz, 3amc, 3amd - TmEndo-Gase – Thermotoga maritima 3o7o, 3amg - TmEndo-Gase (mutant) 3mmu, 3mmw - Endo-Gase 5A 1ga2, 1g87 - CcEndo-Gase 9G 8a3h, 7a3h, 1a3h - BaEndo-Gase 5A catalytic domain – Bacillus agaradhaerens 2a39 - HiEndo-Gase I 2eng - HiEndo-Gase V 1dys, 1hd5 - HiEndo-Gase 6B catalytic domain 2bw8, 1h0b - RmEndo-Gase 12A – Rhodothermus marinus 1tvn - PhEndo-Gase G catalytic domain – Pseudoalteromonas haloplanktis 1l8f - MaEndo-Gase – Melanocarpus albomyces 1ks4, 1ks5 - Endo-Gase A – Aspergillus niger 1gzj - Endo-Gase – Thermoascus aurantiacus 1e8p, 1e8q, 2j4m, 2j4n - PeEndo-Gase cellulose docking domain – Piromyces – NMR
1g01 - Endo-Gase catalytic domain – Bacillus 1egz - EchEndo-Gase Z catalytic domain – Erwinia chrysanthemi 1aiw - EchEndo-Gase Z cellulose-binding domain – NMR
3ovw - FoEndo-Gase I – Fusarium oxysporum 2jep - PpEndo-Gase – Paenibacillus pabuli 2yih, 3zq9 - PpoEndo-Gase – Paenibacillus polymyxa 2yjq, 2ykk - PpoEndo-Gase (mutant) 2zew, 2zex - TpEndo-Gase – Thermoanaerobacterium polysaccharolyticus 2zez - TpEndo-Gase carbohydrate-binding module 2 3ayr - PrEndo-Gase – Piromyces rhizinflatus 2uwa - NaEndo-Gase – Nasturium 2uwb - NaEndo-Gase (mutant) 3ez8 - Endo-Gase – Alicyclobacillus acidocaldarius 3fw6, 3ii1 - Endo-Gase – uncultured bacterium


Endo-1,4 – β-glucanase binary complex

3ndz - CcEndo-Gase D catalytic and cellulose-binding domains + polysaccharide
1kfg - CcEndo-Gase G catalytic domain + polysaccharide
1f9d, 1fbw - CcEndo-Gase F catalytic domain (mutant) + polysaccharide
1f9o, 1fae, 1fbo - CcEndo-Gase F catalytic domain + polysaccharide
1k72, 1kfg - CcEndo-Gase 9G + polysaccharide
2wao - CtEndo-Gase E C terminal domain + polysaccharide
2vi0, 2cit - CtEndo-Gase H catalytic domain (mutant) + polysaccharide
2bvd - CtEndo-Gase H catalytic domain + polysaccharide
1cen - CtEndo-Gase catalytic domain (mutant) + polysaccharide
3qxq, 2jen - EcEndo-Gase (mutant) + polysaccharide
3amm - TmEndo-Gase + polysaccharide
3amn, 3amp, 3amq - TmEndo-Gase (mutant) + polysaccharide
2bod, 2bof, 2bog - TfEndo-Gase E2 catalytic domain + polysaccharide
1w3l, 1ocq, 1h2j, 1h11, 1h5v, 1hf6, 1e5j - BaEndo-Gase 5A catalytic domain + polysaccharide
2v38, 1ocq - BaEndo-Gase 5A catalytic domain + drug 1qi0, 1qi2 - BaEndo-Gase B catalytic domain + polysaccharide
1u0a - Endo-Gase (mutant) + polysaccharide
– Paenibacillus macerans
3acg, 3ach - CjEndo-Gase 5A residues 560-752 + polysaccharide
1uu4, 1uu5, 1uu6, 1w2u - HgEndo-Gase 12A catalytic domain + polysaccharide
1j84 - CcEndo-Gase ENGF + polysaccharide
– Clostridium cellulovorans
3tf4, 4tf4 - TfEndo-Gase E4 catalytic domain + polysaccharide
1ece - Endo-Gase E1 catalytic domain + polysaccharide
– Acidothermus cellulolyticus 2vn5, 2vn6 - CcEndo-Gase A residues 410-475 + cohesin residues 277-427 3pzt - BsEndo-Gase catalytic domain + Mn 3rjy – FnEndo-Gase + glucose 3vlb - AaEndo-Gase + protein EDGP

Endo-1,3-1,4–β-glucanase binary complex

3axe - FsEndo-Gase (mutant) + polysaccharide

Non-specific endo-glucanase binary complex

1kwf - CtEndo-Gase A catalytic domain (mutant) + polysaccharide
3aof, 3azr, 3azs, 3azt - TmEndo-Gase (mutant) + polysaccharide
5a3h, 6a3h, 4a3h, 2a3h, 3a3h - BaEndo-Gase 5A catalytic domain + polysaccharide
2bwa, 2bwc - RmEndo-Gase 12A + polysaccharide
1tvp - PhEndo-Gase G catalytic domain + polysaccharide
1oa7 - MaEndo-Gase + polysaccharide
1g0c - Endo-Gase catalytic domain + polysaccharide – Bacillus
2nlr - SlEndo-Gase catalytic domain + polysaccharide
2ovw, 4ovw, 1ovw - FoEndo-Gase I + polysaccharide
3eng, 4eng - HiEndo-Gase V catalytic domain + polysaccharide
1uoz, 1up0, 1up2, 1up3 - Endo-Gase + polysaccharide – Mycobacterium tuberculosis
2jeq - PpEndo-Gase + polysaccharide
2zey - TpEndo-Gase + polysaccharide
3ays - PrEndo-Gase (mutant) + polysaccharide
3oea, 3oeb - Endo-Gase (mutant) + polysaccharide – Caldanaerobius polysaccharolyticus
3nco - FnEndo-Gase + peptide

Exo-1,3-β-glucanase

3o6a - CaExo-Gase (mutant) – Candida albicans
1eqp, 1cz1 - CaExo-Gase
1ex1 - bExo-Gase
1h4p - Exo-Gase – yeast

Exo-1,4-β-glucanase

1ut9 - CtExo-Gase catalytic domain
1q2b, 1egn, 2cel, 4cel - HjExo-Gase I catalytic domain (mutant)
1h46, 1gpi - PcExo-Gase I catalytic domain – Phanerochaete chrysosporium

Exo-1,3-1,4-β-glucanase

3f95 - PsExo-Gase C terminal domain – Pseudoalteromonas
3rrx - PsExo-Gase (mutant)
3usz, 3ut0 - PsExo-Gase

Exo-1,3-β-glucanase binary complex

1eqc - CaExo-Gase + inhibitor
3n9k– CaExo-Gase (mutant) + polysaccharide

Exo-1,4-β-glucanase binary complex

3cuf, 3cug, 3cuh, 3cui, 3cuj - CfExo-Gase + polysaccharide
2v3i, 2v3r - HjExo-Gase I catalytic domain + phenanthrenolol
1q2e, 5cel, 6cel, 7cel, 3cel - HjExo-Gase I catalytic domain (mutant) + polysaccharide
1dy4 - TrExo-Gase I catalytic domain + propranolol

β-1,3-glucanase (BGase)

2jon – BGase C terminal – olive – NMR
3eqn – PcBGase
3eqo – PcBGase + gluconolactone
3gdo – SmBGase – Streptomyces matensis
3gd9 - SmBGase + polysaccharide
1byh – BGase/AGase – synthetic

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

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