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Glucanase
From Proteopedia
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[[3atg]] - Endo-Gase – ''Cellulosimicrobium cellulans''<br /> | [[3atg]] - Endo-Gase – ''Cellulosimicrobium cellulans''<br /> | ||
[[3dgt]] - Endo-Gase catalytic domain – ''Streptomyces sioyaensis''<br /> | [[3dgt]] - Endo-Gase catalytic domain – ''Streptomyces sioyaensis''<br /> | ||
| - | [[3f55]], [[3em5]] - Endo-Gase – ''Hevea brasiliensis''<br /> | + | [[3f55]], [[3em5]], [[4hpg]], [[4iis]] - Endo-Gase – ''Hevea brasiliensis''<br /> |
[[2vy0]] - Endo-Gase residues 35-297 – ''Pyrococcus furiosus''<br /> | [[2vy0]] - Endo-Gase residues 35-297 – ''Pyrococcus furiosus''<br /> | ||
[[2hyk]] - Endo-Gase – ''Nococardiopsis''<br /> | [[2hyk]] - Endo-Gase – ''Nococardiopsis''<br /> | ||
[[2cyg]] - Endo-Gase – ''Musa acuminate''<br /> | [[2cyg]] - Endo-Gase – ''Musa acuminate''<br /> | ||
| + | [[4k35]], [[4k3a]] - Endo-Gase – ''Rhizomucor miehei''<br /> | ||
[[4dfs]] - Endo-Gase catalytic domain – ''Thermotoga petrophila''<br /> | [[4dfs]] - Endo-Gase catalytic domain – ''Thermotoga petrophila''<br /> | ||
[[2cl2]] - Endo-Gase – ''Phanerochaete chrysosporium''<br /> | [[2cl2]] - Endo-Gase – ''Phanerochaete chrysosporium''<br /> | ||
[[3ur7]], [[3ur8]] - pEndo-Gase – potato<br /> | [[3ur7]], [[3ur8]] - pEndo-Gase – potato<br /> | ||
| + | [[4bow]] - ZgEndo-Gase catalytic domain (mutant) – ''Zobellia galactanivorans''<br /> | ||
| + | [[4bq1]] - ZgEndo-Gase catalytic domain <br /> | ||
| + | [[4bpz]] - ZgEndo-Gase catalytic domain (mutant) + trisasccharide<br /> | ||
===Endo-1,4-β-glucanase=== | ===Endo-1,4-β-glucanase=== | ||
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[[3ik2]] - Endo-Gase A residues 34-541 – ''Clostridium acetobulylicum''<br /> | [[3ik2]] - Endo-Gase A residues 34-541 – ''Clostridium acetobulylicum''<br /> | ||
[[2xfg]] - CtEndo-Gase 1 catalytic and cellulose-binding domains – ''Clostridium thermocellum''<br /> | [[2xfg]] - CtEndo-Gase 1 catalytic and cellulose-binding domains – ''Clostridium thermocellum''<br /> | ||
| - | [[2v3g]], [[2cip]], [[2bv9]] - CtEndo-Gase H catalytic domain<br /> | + | [[2lro]], [[2lrp]] - CtEndo-Gase CBM11 domain – NMR<br /> |
| + | [[2v3g]], [[2cip]], [[2bv9]], [[4im4]] - CtEndo-Gase H catalytic domain<br /> | ||
[[1cem]], [[2e0p]], [[1is9]] - CtEndo-Gase A catalytic domain<br /> | [[1cem]], [[2e0p]], [[1is9]] - CtEndo-Gase A catalytic domain<br /> | ||
[[2wab]] - CtEndo-Gase E C terminal domain (mutant) <br /> | [[2wab]] - CtEndo-Gase E C terminal domain (mutant) <br /> | ||
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[[1qhz]] - BaEndo-Gase B catalytic domain<br /> | [[1qhz]] - BaEndo-Gase B catalytic domain<br /> | ||
[[2ckr]], [[2cks]] - TfEndo-Gase E5 catalytic domain - ''Thermobifida fusca''<br /> | [[2ckr]], [[2cks]] - TfEndo-Gase E5 catalytic domain - ''Thermobifida fusca''<br /> | ||
| + | [[3rpt]] - TfEndo-Gase<br /> | ||
[[2boe]] - TfEndo-Gase E2 catalytic domain (mutant) <br /> | [[2boe]] - TfEndo-Gase E2 catalytic domain (mutant) <br /> | ||
[[3prt]] - TfEndo-Gase E2 catalytic domain<br /> | [[3prt]] - TfEndo-Gase E2 catalytic domain<br /> | ||
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[[4doe]] - CbEndo-Gase GH9 module + ligand<br /> | [[4doe]] - CbEndo-Gase GH9 module + ligand<br /> | ||
[[4h7m]] - CbEndo-Gase - ''Trichoderma harzianum''<br /> | [[4h7m]] - CbEndo-Gase - ''Trichoderma harzianum''<br /> | ||
| + | [[3wc3]] - Endo-Gase – ''Eisenia fetida''<br /> | ||
===Endo-1,3-1,4-β-glucanase=== | ===Endo-1,3-1,4-β-glucanase=== | ||
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[[3gdo]] – SmBGase – ''Streptomyces matensis''<br /> | [[3gdo]] – SmBGase – ''Streptomyces matensis''<br /> | ||
[[3gd9]] - SmBGase + polysaccharide<br /> | [[3gd9]] - SmBGase + polysaccharide<br /> | ||
| - | [[1byh]] – BGase/AGase – synthetic | + | [[1byh]] – BGase/AGase – synthetic<br /> |
| + | [[4le3]] – PaBgase – ''Podospora anserina''<br /> | ||
| + | [[4le4]] – PaBgase + cellotriose<br /> | ||
Revision as of 12:05, 10 March 2014
Template:STRUCTURE 3atg
Glucanases (Gase) are enzymes which break down glucans which are part of plant cell wall. Gase hydrolyzes the 1,4-β-glycosidic link in cellulose, lichenin and β-D-glucan. Endo-Gase breaks internal cellulose bonds. Exo-Gase cleaves 2 to 4 units from the ends of exposed cellulose polysaccharide chains. Gase are classified according to polysaccharide bond which they break. details in Molecular Playground/1,3-1,4-beta-D-glucanase.
3D structures of glucanase
Updated on 10-March-2014
Endo-1,3-β-glucanase
3atg - Endo-Gase – Cellulosimicrobium cellulans
3dgt - Endo-Gase catalytic domain – Streptomyces sioyaensis
3f55, 3em5, 4hpg, 4iis - Endo-Gase – Hevea brasiliensis
2vy0 - Endo-Gase residues 35-297 – Pyrococcus furiosus
2hyk - Endo-Gase – Nococardiopsis
2cyg - Endo-Gase – Musa acuminate
4k35, 4k3a - Endo-Gase – Rhizomucor miehei
4dfs - Endo-Gase catalytic domain – Thermotoga petrophila
2cl2 - Endo-Gase – Phanerochaete chrysosporium
3ur7, 3ur8 - pEndo-Gase – potato
4bow - ZgEndo-Gase catalytic domain (mutant) – Zobellia galactanivorans
4bq1 - ZgEndo-Gase catalytic domain
4bpz - ZgEndo-Gase catalytic domain (mutant) + trisasccharide
Endo-1,4-β-glucanase
3pzu, 3pzv – BsEndo-Gase catalytic domain – Bacillus subtilis
2l8a - BsEndo-Gase CRM3 domain
3ndy - CcEndo-Gase D catalytic and cellulose-binding domains – Clostridium cellulovorans
1j83 - CcEndo-Gase ENGF
3ik2 - Endo-Gase A residues 34-541 – Clostridium acetobulylicum
2xfg - CtEndo-Gase 1 catalytic and cellulose-binding domains – Clostridium thermocellum
2lro, 2lrp - CtEndo-Gase CBM11 domain – NMR
2v3g, 2cip, 2bv9, 4im4 - CtEndo-Gase H catalytic domain
1cem, 2e0p, 1is9 - CtEndo-Gase A catalytic domain
2wab - CtEndo-Gase E C terminal domain (mutant)
1v0a - CtEndo-Gase H
1ceo - CtEndo-Gase C (mutant)
1cec - CtEndo-Gase C
2eqd, 2cn3, 2jem - CtEndo-Gase (mutant)
2eo7, 2cn2 - CtEndo-Gase
3qxf - EcEndo-Gase – Escherichia coli
3amh, 1vho - TmEndo-Gase – Thermotoga maritima
3vhn, 3vho, 3vhp - TmEndo-Gase (mutant)
2qno - CcEndo-Gase F catalytic domain – Clostridium cellulolyticum
1edg - CcEndo-Gase A catalytic domain
2v38, 1w3k - BaEndo-Gase 5A catalytic domain – Bacillus agaradhaerens
1qhz - BaEndo-Gase B catalytic domain
2ckr, 2cks - TfEndo-Gase E5 catalytic domain - Thermobifida fusca
3rpt - TfEndo-Gase
2boe - TfEndo-Gase E2 catalytic domain (mutant)
3prt - TfEndo-Gase E2 catalytic domain
1js4, 1tf4 - TfEndo-Gase E4 catalytic domain
1tml - TfEndo-Gase catalytic domain
1wc2 - Endo-Gase – Mytilus edulis
1uww - Endo-Gase – Bacillus akibai
1oji, 1ojj, 1ojk - Endo-Gase (mutant) – Humicola oryzae
1gu3 - CfEndo-Gase C – Cellulomonas fimi
1ulo, 1cx1 - CfEndo-Gase C cellulose-binding domain
1ulp - CfEndo-Gase C cellulose-binding domain - NMR
1dym, 1a39 - HiEndo-Gase I (mutant) – Humicola insolens
1nlr - SlEndo-Gase catalytic domain – Streptomyces lividans
1eg1, 1olq, 1oa2, 1oa3, 1oa4 - HjEndo-Gase catalytic domain (mutant) – Hypocrea jecorina
3qr3, 1h8v - HjEndo-Gase catalytic domain
4bmf - HjEndo-Gase cellulose-binding domain – NMR
3aci, 3acf - CjEndo-Gase 5A residues 560-752 – Clostridium josui
1olr - HgEndo-Gase 12A catalytic domain – Humicola grisea
1oa9 - MaEndo-Gase – Melanocarpus albomyces
1ks8, 1ksc, 1ksd - Endo-Gase catalytic domain – termite
1h1n - Endo-Gase – Thermoascus aurantiacus
3a0f - Endo-Gase – Geotrichum
3rjx - FnEndo-Gase – Fervidobacterium nodosum
3vl8, 3vl9 - AaEndo-Gase – Aspergillus aculeatus
4dod - CbEndo-Gase GH9 module – Caldicellulosiruptor bescii
4doe - CbEndo-Gase GH9 module + ligand
4h7m - CbEndo-Gase - Trichoderma harzianum
3wc3 - Endo-Gase – Eisenia fetida
Endo-1,3-1,4-β-glucanase
3o5s - BsEndo-Gase catalytic domain
3i4i - Endo-Gase residues 26-242 – Bacterium
3d6e - BlEndo-Gase (mutant) – Bacillus licheniformis
3h0o, 3hr9, 2r49, 3axd - FsEndo-Gase (mutant) – Fibrobacter succinogenes
1zm1, 1mve - FsEndo-Gase
1aq0 - bEndo-Gase – barley
1gbg - BlEndo-Gase –
1glh - Endo-Gase – synthetic
1ghr, 1ghs - Endo-Gase – Hordeum vulgare
1mac – Gase – Paenibacillus macerans
Non-specific endo-glucanase
1clc - CtEndo-Gase D
1l1y, 1l2a - CtEndo-Gase SS
1daq, 1dav - CtEndo-Gase SS dockerin domain - NMR
2eex, 2ej1 - CtEndo-Gase (mutant)
2c24, 2e4t - CtEndo-Gase
2c26 - CtEndo-Gase PKD and CBM44 domains
2c4x - CtEndo-Gase PKD and CBM44 domains (mutant)
3isx, 2fvg, 1vjz, 3amc, 3amd - TmEndo-Gase – Thermotoga maritima
3o7o, 3amg - TmEndo-Gase (mutant)
3mmu, 3mmw - Endo-Gase 5A
1ga2, 1g87 - CcEndo-Gase 9G
8a3h, 7a3h, 1a3h - BaEndo-Gase 5A catalytic domain – Bacillus agaradhaerens
2a39 - HiEndo-Gase I
2eng - HiEndo-Gase V
1dys, 1hd5 - HiEndo-Gase 6B catalytic domain
2bw8, 1h0b - RmEndo-Gase 12A – Rhodothermus marinus
1tvn - PhEndo-Gase G catalytic domain – Pseudoalteromonas haloplanktis
1l8f - MaEndo-Gase – Melanocarpus albomyces
1ks4, 1ks5 - Endo-Gase A – Aspergillus niger
1gzj - Endo-Gase – Thermoascus aurantiacus
1e8p, 1e8q, 2j4m, 2j4n - PeEndo-Gase cellulose docking domain – Piromyces – NMR
1g01 - BaEndo-Gase catalytic domain – Bacillus
1egz - EchEndo-Gase Z catalytic domain – Erwinia chrysanthemi
1aiw - EchEndo-Gase Z cellulose-binding domain – NMR
3ovw - FoEndo-Gase I – Fusarium oxysporum
2jep - PpEndo-Gase – Paenibacillus pabuli
2yih, 3zq9 - PpoEndo-Gase – Paenibacillus polymyxa
2yjq, 2ykk - PpoEndo-Gase (mutant)
2zew, 2zex - TpEndo-Gase – Thermoanaerobacterium polysaccharolyticus
2zez - TpEndo-Gase carbohydrate-binding module 2
3ayr - PrEndo-Gase – Piromyces rhizinflatus
2uwa - NaEndo-Gase – Nasturium
2uwb - NaEndo-Gase (mutant)
3ez8 - Endo-Gase – Alicyclobacillus acidocaldarius
3fw6, 3ii1, 4ee9 - Endo-Gase – uncultured bacterium
4afm - EucEndo-Gase Cel5a N terminal – Eubacterium cellulosolvens
4ba6 - EucEndo-Gase Cel5a C terminal
4aek, 4aem - EucEndo-Gase carbohydrate-binding domain
Endo-1,4–β-glucanase binary complex
3ndz - CcEndo-Gase D catalytic and cellulose-binding domains + polysaccharide
1kfg - CcEndo-Gase G catalytic domain + polysaccharide
1f9d, 1fbw - CcEndo-Gase F catalytic domain (mutant) + polysaccharide
1f9o, 1fae, 1fbo - CcEndo-Gase F catalytic domain + polysaccharide
1k72, 1kfg - CcEndo-Gase 9G + polysaccharide
2wao - CtEndo-Gase E C terminal domain + polysaccharide
2vi0, 2cit - CtEndo-Gase H catalytic domain (mutant) + polysaccharide
2bvd - CtEndo-Gase H catalytic domain + polysaccharide
1cen - CtEndo-Gase catalytic domain (mutant) + polysaccharide
3qxq, 2jen - EcEndo-Gase (mutant) + polysaccharide
3amm - TmEndo-Gase + polysaccharide
3amn, 3amp, 3amq - TmEndo-Gase (mutant) + polysaccharide
2bod, 2bof, 2bog - TfEndo-Gase E2 catalytic domain + polysaccharide
1w3l, 1ocq, 1h2j, 1h11, 1h5v, 1hf6, 1e5j - BaEndo-Gase 5A catalytic domain + polysaccharide
2v38, 1ocq - BaEndo-Gase 5A catalytic domain + drug
1qi0, 1qi2 - BaEndo-Gase B catalytic domain + polysaccharide
1u0a - Endo-Gase (mutant) + polysaccharide – Paenibacillus macerans
3acg, 3ach - CjEndo-Gase 5A residues 560-752 + polysaccharide
1uu4, 1uu5, 1uu6, 1w2u - HgEndo-Gase 12A catalytic domain + polysaccharide
1j84 - CcEndo-Gase ENGF + polysaccharide
3tf4, 4tf4 - TfEndo-Gase E4 catalytic domain + polysaccharide
1ece - Endo-Gase E1 catalytic domain + polysaccharide – Acidothermus cellulolyticus
2vn5, 2vn6 - CcEndo-Gase A residues 410-475 + cohesin residues 277-427
3pzt - BsEndo-Gase catalytic domain + Mn
3rjy – FnEndo-Gase + glucose
3vlb - AaEndo-Gase + protein EDGP
Endo-1,3 – β-glucanase binary complex
4gzi, 4gzj - pEndo-Gase (mutant) + glucose
Endo-1,3-1,4–β-glucanase binary complex
3axe - FsEndo-Gase (mutant) + polysaccharide
Non-specific endo-glucanase binary complex
1kwf - CtEndo-Gase A catalytic domain (mutant) + polysaccharide
3aof, 3azr, 3azs, 3azt - TmEndo-Gase (mutant) + polysaccharide
5a3h, 6a3h, 4a3h, 2a3h, 3a3h - BaEndo-Gase 5A catalytic domain + polysaccharide
2bwa, 2bwc - RmEndo-Gase 12A + polysaccharide
1tvp - PhEndo-Gase G catalytic domain + polysaccharide
1oa7 - MaEndo-Gase + polysaccharide
1g0c - BaEndo-Gase catalytic domain + polysaccharide
2nlr - SlEndo-Gase catalytic domain + polysaccharide
2ovw, 4ovw, 1ovw - FoEndo-Gase I + polysaccharide
3eng, 4eng - HiEndo-Gase V catalytic domain + polysaccharide
1uoz, 1up0, 1up2, 1up3 - Endo-Gase + polysaccharide – Mycobacterium tuberculosis
2jeq - PpEndo-Gase + polysaccharide
2zey - TpEndo-Gase + polysaccharide
3ays - PrEndo-Gase (mutant) + polysaccharide
3oea, 3oeb - Endo-Gase (mutant) + polysaccharide – Caldanaerobius polysaccharolyticus
3nco - FnEndo-Gase + peptide
4afd - Endo-Gase Cel5a N terminal + polysaccharide
Exo-1,3-β-glucanase
3o6a - CaExo-Gase (mutant) – Candida albicans
1eqp, 1cz1 - CaExo-Gase
1ex1 - bExo-Gase
1h4p - Exo-Gase – yeast
Exo-1,4-β-glucanase
1ut9 - CtExo-Gase catalytic domain
1q2b, 1egn, 2cel, 4cel - HjExo-Gase I catalytic domain (mutant)
1h46, 1gpi - PcExo-Gase I catalytic domain – Phanerochaete chrysosporium
Exo-1,3-1,4-β-glucanase
3f95 - PsExo-Gase C terminal domain – Pseudoalteromonas
3rrx - PsExo-Gase (mutant)
3usz, 3ut0 - PsExo-Gase
Exo-1,3-β-glucanase binary complex
1eqc - CaExo-Gase + inhibitor
3n9k– CaExo-Gase (mutant) + polysaccharide
Exo-1,4-β-glucanase binary complex
3cuf, 3cug, 3cuh, 3cui, 3cuj - CfExo-Gase + polysaccharide
2v3i, 2v3r - HjExo-Gase I catalytic domain + phenanthrenolol
1q2e, 5cel, 6cel, 7cel, 3cel - HjExo-Gase I catalytic domain (mutant) + polysaccharide
1dy4 - HjExo-Gase I catalytic domain + propranolol
β-1,3-glucanase (BGase)
2jon – BGase C terminal – olive – NMR
3eqn – PcBGase
3eqo – PcBGase + gluconolactone
3gdo – SmBGase – Streptomyces matensis
3gd9 - SmBGase + polysaccharide
1byh – BGase/AGase – synthetic
4le3 – PaBgase – Podospora anserina
4le4 – PaBgase + cellotriose
